Oxidative nanopatterning of titanium surfaces promotes production and extracellular accumulation of osteopontin

Abstract

The bone-biomaterial interface has been characterized by layers of afibrillar extracellular matrix (ECM) enriched in non collagenous proteins, including osteopontin (OPN), a multifunctional protein that in bone controls cell adhesion and ECM mineralization. Physical and chemical aspects of biomaterial surfaces have been demonstrated to affect cell-ECM-substrate interactions. The present paper described the ability of oxidative nanopatterning of titanium (Ti) surfaces to control extracellular OPN deposition in vitro. Ti discs were chemically treated by a mixture of H 2 SO 4 /H 2 O 2 for either 30 min [Nano(30') Ti] or 4 h [Nano(4h) Ti]. Non-etched Ti discs were used as control. Primary osteogenic cells derived from newborn rat calvarial bone were plated on control and etched Ti and grown under osteogenic conditions up to 7 days. High resolution scanning electron microscopy revealed that treated Ti discs exhibited a nanoporous surface and that areas of larger nanopits were noticed only for Nano(4h) Ti. Large extracellular OPN accumulation were detectable only for Nano(4h) Ti, which was associated with OPN-positive cells with typical aspects of migrating cells. At day 3, quantitative results in terms of areas of OPN labeling were as follows: Nano(4h) Ti > Nano(30') Ti > Control Ti. In conclusion, chemically nanostructured Ti surfaces may support the enhancement of endogenous extracellular OPN deposition by osteogenic cells in vitro depending on the etching time, a finding that should be taken into consideration in strategies to biofunctionalize implant surfaces with molecules with cell adhesion capacity.

Rates of hospitalized bacterial infection associated with juvenile idiopathic arthritis and its treatment

“…A well‐constructed SC provides a suitable surface for cells to attach and adhere with a porous and well interconnected network guiding the development of new bone, supporting migration, proliferation, and differentiation of bone‐forming cells, and vascularization of the ingrowth tissue 1–3. Although several polymers and bioceramics have been developed for their use in bone tissue engineering, their low mechanical properties have limited their use for load‐bearing applications 4, 5…”

The effect of collagen coating on titanium with nanotopography on in vitro osteogenesis

“…Surface modifications that result in nanostructured patterns may modulate osteoblast responses from early to late stages of differentiation . A specific nanotopographic surface can be obtained using a simple method based on controlled surface oxidation through chemical conditioning with H 2 SO 4 /H 2 O 2 solution .…”

“…A specific nanotopographic surface can be obtained using a simple method based on controlled surface oxidation through chemical conditioning with H 2 SO 4 /H 2 O 2 solution . Our previous results showed that this Ti surface increases the osteogenic potential of osteoblastic cells and promotes osteoblast differentiation under noninducing conditions through modulation of a circuit involving bone morphogenetic protein (BMP) and integrin signaling . Furthermore, this Ti surface with nanotopography promotes in vivo bone formation in close contact with dental implants …”

“…7 Surface modifications that result in nanostructured patterns may modulate osteoblast responses from early to late stages of differentiation. [8][9][10][11] A specific nanotopographic surface can be obtained using a simple method based on controlled surface oxidation through chemical conditioning with H 2 SO 4 /H 2 O 2 solution. 5,10 Our previous results showed that this Ti surface increases the osteogenic potential of osteoblastic cells and promotes osteoblast differentiation under noninducing conditions through modulation of a circuit involving bone morphogenetic protein (BMP) and integrin signaling.…”

“…5,10 Our previous results showed that this Ti surface increases the osteogenic potential of osteoblastic cells and promotes osteoblast differentiation under noninducing conditions through modulation of a circuit involving bone morphogenetic protein (BMP) and integrin signaling. 5,[11][12][13][14][15] Furthermore, this Ti surface with nanotopography promotes in vivo bone formation in close contact with dental implants. 16 Among biochemical Ti modifications, the immobilization of collagen type 1 is one of the most promising approach since this protein constitutes 95% of the organic matrix of the bone tissue and mediates several osteoblastic activities.…”