Cytotoxicity of latex and non-latex orthodontic elastomeric ligatures on L929 mouse fibroblasts

Santos, Rogério Lacerda dos; Pithon, Matheus Melo; Martins, Fernanda Otaviano; Romanos, Maria Teresa Villela; Ruellas, Antônio Carlos de Oliveira

doi:10.1590/s0103-64402010000300005

Cited by 19 publications

(23 citation statements)

References 20 publications

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“…Although the acrylic resins tested by different methods presented over 50% of cell viability in all the experimental time intervals (Table 2), which does not contraindicate their clinical use, as seen in other studies (20,24) on cytotoxicity of materials (25) for use in dentistry that showed similar performance. However, the findings of this study suggest that the mass technique associated with manual polishing must be encouraged whenever possible, but the saturation technique associated with chemical polishing seems to be less suitable.…”

Section: Discussionsupporting

confidence: 69%

Mechanical and Biological Properties of Acrylic Resins Manipulated and Polished by Different Methods

et al. 2013

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This study evaluated the influence of the manipulation technique and polishing method on the flexural strength and cytotoxicity of acrylic resins. Two manipulation techniques and three polishing methods were used in the fabrication of acrylic plates that were divided into 6 groups (n=10). Groups MM, MC and MW: mass technique with mechanical polishing, chemical polishing and without polishing, respectively; and Groups SM, SC and SW: Saturation technique with mechanical polishing, chemical polishing and without polishing, respectively). Flexural strength was tested in a universal testing machine and the cytotoxicity assay used cell cultures (L-929) for periods of 24 h to 168 h. Flexural strength and cytotoxicity data were assessed using two-way and three-way ANOVA, respectively (a=0.05), followed by post hoc Bonferroni test for multiple comparisons. The effect of combinations of manipulation techniques and polishing methods on flexural strength showed significant differences only between Group SC and Groups MW, MM and MC (p<0.01). Cell viability ranged from 51% (3.9%) to 87,6% (3.2) in the 24-h time interval, and from 87.8% (5.0) to 95.7% (3.1%) in the 168-h time interval. With the increase of cell viability, from the third day (72 h), there was no significant difference among the groups, except between MM and SC (p<0.01) at 72 h. In conclusion, the manipulation technique and polishing method had more influence on the cytotoxicity than on flexural strength.

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Section: Discussionsupporting

confidence: 69%

Mechanical and Biological Properties of Acrylic Resins Manipulated and Polished by Different Methods

et al. 2013

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“…In this study, neutral red dye was used because it is widely used for identification of L-929 cell viability. 3,4 Dead or damaged cells cannot incorporate vital stain and are thus not recognized on optical reading. Therefore, spectrophotometry does not allow dead cells to be distinguished from the damaged ones.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, the elastics were superficially washed with deionized water (Millipore, Bedford, Mass) for 5 seconds and sterilized on both sides with ultraviolet light (Labconco, Kansas City, Mo) for 30 minutes. [3][4][5]18 To verify the cell response to extreme situations, another three groups were included in the study: group cell control (CC), consisting of cells not exposed to any material; group positive control (C+), consisting of Tween 80; and group (negative control (C2), consisting of phosphate-buffered saline (PBS) solution in contact with the cells.…”

Section: Cytotoxicity Testmentioning

confidence: 99%

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The influence of pH levels on mechanical and biological properties of nonlatex and latex elastics

Santos¹,

Pithon²,

Romanos³

2012

The Angle Orthodontist

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Objective: To evaluate the influence of pH levels on interarch elastics with regard to force decay and cytotoxicity. Materials and Methods: One nonlatex (NLAO) group and one latex (LAO) group were tested (n 5 10). Elastics were stretched to 25 mm and were held for 1, 6, 12, and 24 hours in artificial saliva solutions with pH levels of 5.0, 6.0, and 7.5. Force magnitudes were measured at 25 mm of activation. The cytotoxicity assay was performed using cell cultures (L929 mouse fibroblast cell line), which were subjected to the cell viability test with neutral red (''dye-uptake''). Force decay and cytotoxicity were assessed using analysis of variance, the Sidak method, and a Tukey's test. Results: The interactions between group, pH, and time showed no statistically significant differences (P 5 .29). When pH per time (P 5 .032) and group per time (P 5 .0009) were considered, these interactions showed statistically significant differences (P , .05). The pH did not interfere directly in the degradation results of the tested elastics. The cytotoxicity test showed that group LAO presented lower cell viability when compared with group NLAO over the course of the entire experiment. There was a gradual reduction in cell viability from 1 hour to 24 hours. A significant difference (P , .05) was found between the interactions group pH and the control group of cells, except between group NLAO at the time point of 1 hour at different pH values and at the time points of 6 and 12 hours with pH 5 (P . .05). Conclusions: No significant correlation between pH, force decay, and cytotoxicity was observed. (Angle Orthod. 2012;82:709-714.)

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“…Elastomeric chains have been widely used in Orthodontics since the 1960s and its effects has been evaluated since then (1)(2)(3)(4)(5). They are used to deliver continuous light forces during sliding mechanics in diastema closure (6), rotational correction, and mainly during canine retraction after premolar extraction (7,8).…”

Section: Introductionmentioning

confidence: 99%

In situ evaluation of orthodontic elastomeric chains

et al. 2012

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The hypothesis tested in this study was that intraoral exposure of elastomeric chains alters their tensile strength. For such purpose, it was evaluated the in situ behavior of different elastomeric chains stretched for 3 weeks. Three kinds of elastomeric chains, Plastic chain (PC), Memory chain (MC) and Super slick chain (SSC), were randomly placed in 3 quadrants of 13 patient in a fixed distance of 16 mm and mean initial force of 180 g. Tensile testing was performed in an universal testing machine at different intervals: initial, 1 h, 24 h, 1 week, 2 weeks and 3 weeks. A two-way ANOVA test was performed to identify the influence of both material and time on the force decrease. A subsequent one-way ANOVA test with the Tukey's post hoc test was used to identify statistically significant intragroup and intergroup remaining force (g and %) differences at 5% significance level. The effect of both the material and the time factors were significant. All groups showed significant force decrease after the 1-h period (23% for PC and 14% for MC and SSC). At the end of the 3-week period, the remaining force was 57% (96 g), 67% (129 g) and 71% (125 g) for PC, MC and SSC, respectively. In conclusion, intraoral exposure of elastomeric chains altered their tensile strength. In general, the greater force decrease occurred within the first hour. The remaining force of the enhanced chains measured at each time interval was greater than the conventional one (PC). After 3 weeks, only the enhanced chains maintained the force applied over 100 g.

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Cytotoxicity of latex and non-latex orthodontic elastomeric ligatures on L929 mouse fibroblasts

Cited by 19 publications

References 20 publications

Mechanical and Biological Properties of Acrylic Resins Manipulated and Polished by Different Methods

Mechanical and Biological Properties of Acrylic Resins Manipulated and Polished by Different Methods

The influence of pH levels on mechanical and biological properties of nonlatex and latex elastics

In situ evaluation of orthodontic elastomeric chains

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