Antimicrobial activity of calcium hydroxide pastes on Enterococcus faecalis cultivated in Root Canal Systems

Abstract

The effectiveness of calcium hydroxide pastes: Calen™ and PMCC-Calen™ associated to chemo-mechanical preparation was assessed on Enterococcus faecalis grown within root canals. Seventy incisors were inserted into TSB medium, sterilized and contaminated with E. faecalis. Culture medium was replaced each 24 h and incubated at 37 o C for 72 h. After chemo-mechanical preparation, root canals were filled with Calen™ or PMCC-Calen™ (7 or 14 days). Pastes were removed and teeth were inserted into test tubes containing Enterococcosel broth. Calen™ paste (maintained for 7 and 14 days) induced 70% elimination of enterococci and PMCC-Calen™ 100% elimination only after maintenance for 14 days. These medications were significantly more effective (p<0.001) than chemo-mechanical protocol alone and PMCC-Calen™ maintained for 7 days, both incapable to eliminate the viability of enterococci. Calcium hydroxide pastes demonstrated important adjuvant effects in the elimination of enterococci during chemo-mechanical preparation of root canal systems. When associated with PMCC, calcium hydroxide pastes should be maintained for at least 14 days.

The association of chlorhexidine digluconate and calcium chloride to use as vehicle of a silicate calcium-based cement

“…ATCC 29212 strains are originally isolated from urine, but used in standard antimicrobial assays. [18,19] In another study, the authors used ATCC 4082 which was originally isolated from root canals of a pulpless tooth. [20] Chlorhexidine is active against a wide range of microorganisms.…”

An Insight to Occult Caries- An Overview with a Novel Approach in the Management

“…In our case, Calcium hydroxide was used for the revascularization procedure. According to the previous studies, the typical revascularisation protocol advocates placing antimicrobial agents ( metronidazole, ciprofloxacin , minocycline, or metronidazole, ciprofloxacin or cefeclor or metronidazole with ciprofloxacin [12] or calcium hydroxide [13,14]) for canal disinfection followed by a coronal seal with MTA. The problems seen with triple antibiotic paste arecrown discoloration, resistant strains and allergic reactions [12].…”

Study Comparing the Effectiveness of Chlorhexidine, Calcium Hydroxide and Linezolid Based Medicaments Against Enterococcus Faecalis

“…Calcium hydroxide (CH) has been popular as an intra canal medicament due to its additional action on gram negative bacteria [5,6]. Some authors have discussed its effectiveness in eliminating EF [7,8]. Combinations of CH with iodoform have been previously believed to enhance periapical bone regeneration simultaneous with resorption of excess material, with a success rate of 84% to 100% [9].…”