Scanning electron microscopic analysis of the effect of CarisolvTM gel on periodontally compromised human root surfaces

Abstract

The aim of this study was to analyze, under scanning electron microscopy (SEM), the morphologic characteristics of root surfaces after application of Carisolv TM gel in association with scaling and root planing (SRP). Sixty periodontally compromised extracted human teeth were randomly assigned to 6 groups: 1) SRP alone; 2) passive topical application of Carisolv TM + SRP; 3) active topical application of Carisolv TM + SRP; 4) multiple applications of Carisolv TM + SRP; 5) SRP + 24% EDTA; 6) topical application of Carisolv TM + SRP + 24% EDTA. Carisolv TM gel was applied to root surfaces for 30 s, followed by scaling and root planing, consisting of 50 strokes with Gracey curettes in an apical-coronal direction, parallel to the long axis of the tooth. The only exception was group 4, in which the roots were instrumented until a smooth, hard and glass-like surface was achieved. All specimens were further analyzed by SEM. The results showed that the treatment with Carisolv TM caused significant changes in root surface morphology of periodontally compromised teeth only when the chemical agent was actively applied (burnishing technique). Carisolv TM failed to remove the smear layer completely, especially with a single application, independently of the method of application. Multiple applications of Carisolv TM were necessary to achieve a smear layer reduction comparable to that obtained with 24% EDTA conditioning.

Comparison of the Efficacy of 17% EDTA and 17% EDTA-S as Root Conditioning Agents: An in vitro Study

“…The application of chemo-mechanical therapy resulted in the elimination of the infected cementum along with the exposure of the unaffected cementum, as evidenced by the specimens treated with EDTA-S in the current experiment, which had a mosaic-like appearance showing the cementum. This was consistent with a prior investigation conducted by Grisi et al 17…”

“…16 The application of chemo-mechanical therapy resulted in the elimination of the infected cementum along with the exposure of the unaffected cementum, as evidenced by the specimens treated with EDTA-S in the current experiment, which had a mosaic-like appearance showing the cementum. This was consistent with a prior investigation conducted by Grisi et al 17 Dentinal tubule patency was seen in the EDTA-S group at a magnification of 1500´. The dentinal tubule integrity could be visible even at a magnification of 200´, according to an investigation conducted by Banerjee et al 18 This was not observed in line with research by Grisi et al 17 The dentinal tubules of the EDTA group were found to be less permeable than those of the EDTA-S group at a magnification of 1500´.…”

“…This was consistent with a prior investigation conducted by Grisi et al 17 Dentinal tubule patency was seen in the EDTA-S group at a magnification of 1500´. The dentinal tubule integrity could be visible even at a magnification of 200´, according to an investigation conducted by Banerjee et al 18 This was not observed in line with research by Grisi et al 17 The dentinal tubules of the EDTA group were found to be less permeable than those of the EDTA-S group at a magnification of 1500´. Similar results were seen in the case of EDTA in a research investigation by Amaral et al, who studied the effects of the chemicals phosphoric acid, EDTA, tetracycline, and citric acid on the removal of minerals.…”

Efficacy of Carisolv™ as an adjunctive therapy to scaling and root planing on subgingival calculus removal

“…Additionally, multiple applications of Carisolv™ combined with SRP procedures could remove the contaminated cementum and expose its healthy structure (13).…”