Tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats

Abstract

The treatment of persistent bone defects has encouraged the search for proper techniques or bone substitutes. In Dentistry, a common problem in the treatment of periodontal bone defects is the growth of tissues within the lesion, such as the junctional epithelium, which impair regeneration of these tissues. Guided tissue regeneration (GTR), based on the separation of the tissues by means of membranes or barriers, was developed in an attempt to improve periodontal regeneration. The aim of this study was to histologically evaluate the tissue response to a membrane of demineralized bovine cortical bone implanted in the subcutaneous tissue of rats. The study periods were 1, 3, 7, 15, 30 and 60 days after implantation. Analysis of the histological sections demonstrated a moderate to intense inflammatory response at 1 and 3 days, moderate at 7 and 15 days, and almost absent at 30 and 60 days. Resorption of the membrane began 15 days after implantation, and at 60 days only remnants could be detected in some animals. We concluded that the demineralized bovine cortical bone membrane was well tolerated by the tissues and is completely resorbed after 30-60 days by mononuclear cells and multinucleated giant cells, which disappear upon completion of the process.

Guided bone regeneration following surgical treatment of a rare variant of Pindborg tumor: a case report

“…This barrier consists of demineralized bovine cortical bone, type I collagen, and growth factors. This material has high biocompatibility and resorbs within 30 days, thus eliminating the need for another surgical approach for its removal (10).…”

Exploring anorganic bovine bone granules as osteoblast carriers for bone bioengineering: a study in rat critical-size calvarial defects

“…Regarding the biological model used in this study, it was seen that critical-size calvarial defects have been widely used to assess the osteoconductive/ osteoinductive potential of new biomaterials (2). Previous studies of our research group (4,5) have evaluated these biomaterials implanted in rat subcutaneous tissue in order to evaluate their biocompatibility. In the present study, it was decided to explore rat critical-size calvaria defects based on bone bioengineering aspects, as it is able to promote bone new formation (19).…”

“…There are several approaches to bone tissue engineering, but all involve one or more of the following key ingredients: harvested cells, recombinant signaling molecules, and three-dimensional (3D) matrices. In this way, several graft materials have been used for the restoration of these defects (1)(2)(3)(4)(5). In general, a graft ideally needs to promote bone growth in order to restore a functional tissue.…”

MMP-9 and CD68+ cells are required for tissue remodeling in response to natural hydroxyapatite

“…The implantation of biomaterials in the animal subcutaneous tissue could be considered an essential tool to evaluate the inflammatory process, ECM remodeling (Accorsi-Mendonça et al 2005), tissue repair (Oliveira et al 2004;Zambuzzi et al , 2006a, and ectopic bone formation (Urist 1970;Rittenberg et al 2005).…”