Determination of arsenic species in P. calomelanos and N. biserrata

Abstract

O sítio industrial abandonado na Baía de Sepetiba (SE-Brasil) está entre os maiores passivos ambientais contaminados com Zn no mundo e o As também se encontra em concentrações alarmantes. A vegetação no local é escassa e das espécies que sobrevivem no local Pityrogramma calomelanos e Nephrolepis biserrata são as mais abundantes. Concentrações de As de até 2271 mg kg -1 foram detectadas na espécie Pityrogramma calomelanos, sendo que essa espécie foi mais eficiente que N. Bisserata no acúmulo desse elemento.Utilizou-se cromatografia de exclusão por tamanho (SEC) com detecção UV-Vis e espectrometria de massas com plasma indutivamente acoplado (ICP-MS) em sistema "on line" para monitorar a distribuição de As na fração orgânica. As maiores concentrações de As foram determinadas na fração extraída com TRIS-HCl, pH 7,4 (entre 60-100% da concentração total de As) mostrando uma evidente associação desse elemento com bioligantes, entre eles, fitoquelatinas e glutationa.The abandoned industrial site at the Sepetiba Bay (SE-Brazil) is one of the most heavily contaminated sites on the earth's surface concerned to Zn, but also presents As in alarming concentrations. Vegetation on this site is scarce and under the surviving species Pityrogramma calomelanos and Nephrolepis biserrata are the most abundant ones. Total As concentrations up to 2271 mg kg -1 in P. calomelanos were determined, being this specie more efficient in arsenic accumulation than N. bisserata. The size exclusion chromatography (SEC) with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (ICP-MS) was used to screen the distribution of As between different molar mass fractions. Highest concentration of As was found after extraction with TRIS-HCl, pH 7.4 (about 60-100% of total As) characterizing an association of this element with bioligands including phytochelatins and glutathione.

Trace elements and radionuclides in palm oil, soil, water, and leaves from oil palm plantations: A review

“…Determination of arsenic in seeds, fruits and some researchers , Kukieret al, 1994;Jurgenet al, 2000., Melendez et al, 2011., Ramirez-Andreottaet al, 2013 have studied stem of various plants.The determination of arsenic in water from oil palm plantation is sparse.…”

Atomic spectrometry update. Elemental speciation review

“…Many of these are based on the use of established methodologies and offer no new analytical insight, although the levels reported are oen alarmingly high, for example total As concentrations up to 2271 mg kg À1 in plants (P. calomelanos) grown on an industrial site in Sepetiba Bay area of SE Brazil. 143 In some cases speciation studies are performed, such as the study by Marquez-Garcia et al who used HPLC-ICP-MS to study the As speciation in soils from the Sao Domingos mine area in Portugal. 144 Two metal tolerant plant species: Erica andevalensis, an endemic heather, and Erica australis, a widely distributed species, were studied.…”