Utilization of a novel Ag(III)-luminol chemiluminescence system for determination of d-penicillamine in human urine samples

Abstract

. O desvio padrão relativo é 0,23% para 11 repetições de 0,06 μg mL -1 de d-penicilamina. O método tem sido aplicado com sucesso às determinações de d-penicilamina em preparações farmacêuticas e amostras de urina, proporcionando resultados satisfatórios. Com base nos espectros de quimioluminescência e no experimento de aprisionamento de radicais livres realizado neste trabalho, um possível mecanismo de reação para esse sistema reacional é sugerido.A novel sensitive chemiluminescence (CL) system ([Ag(HIO 6 ) 2 ] 5− -luminol reaction system), coupled with a flow injection, is introduced for determination of d-penicillamine. The system is based on the enhancement effect of chemiluminescence reaction by penicillamine in alkaline medium. Under the optimum conditions, the CL intensity is proportional to the concentrations of d-penicillamine with a good linearity. The linear range covers 1.5-150 ng mL -1 and the limit of detection of the method is 0.8 ng mL -1 . The relative standard deviation is 0.23% for 11 repeated measurements of 0.06 μg mL -1 d-penicillamine. The method has been successfully applied to the determinations of d-penicillamine in pharmaceutical preparations and urine samples, giving rise to satisfactory results. Based on the chemiluminescent spectra and free radical trapping experiment performed in this work, a possible reaction mechanism for this reaction system is suggested.

Highly selective and sensitive determination of several antioxidants in human breast milk using high-performance liquid chromatography based on Ag(III) complex chemiluminescence detection

“…During the last few years, we have focused on the studies of the interactions of [Ag(HIO 6 ) 2 ] 5with bioactive and medically important molecules (Shi, 2009;Ma, Niu, Wang, Kang, & Shi, 2014;Ma, 2012;Ma, Fan, Xu, Kang, & Shi, 2011). We have found that [Ag(HIO 6 ) 2 ] 5can oxidize some reducible substances such as some soluble antioxidants and lead to CL signal enhancement remarkably for the [Ag(HIO 6 ) 2 ] 5-luminol system.…”

Investigation on Ruthenium(II) Bipyridine/AgIII Complexes Chemiluminescence System and Its Application for Sensitive Norfloxacin and Ofloxacin Detection

“…In our previous studies, 12,13 we have been focusing on the interactions between the bis(hydrogenperiodato) argentate(III) complex anion, [Ag(HIO 6 ) 2 ] 5-, and bioactive and medically important molecules in alkaline medium. Based on the Ag-luminol-NaOH CL system, a variety of sensitive detection methods for small biological molecules [14][15][16] and drug molecules 17,18 have been developed. However, the oxidation properties and applications of Ag III in acidic medium require further analysis.…”

A simple method for determination of d-penicillamine on the carbon paste electrode using cupric ions

“…Some of these methods include colorimetry , fluorimetry [5], chromatography [6], flow injection analysis [7], electrophoresis [8], potentiometry [9], voltammetry [10,11], PMR spectrometry [12] and NMR spectrometry [13].…”