Volatile compounds of Baccharis punctulata, Baccharis dracunculifolia and Eupatorium laevigatum obtained using solid phase microextraction and hydrodistillation

Abstract

Neste trabalho foram comparados perfis cromatográficos dos óleos provenientes de hidrodestilação de folhas de Baccharis punctulata, Baccharis dracunculifolia e Eupatorium laevigatum e os perfis cromatográficos obtidos utilizando-se HS-SPME. Várias plantas nativas do Brasil ainda não foram estudadas quanto à sua composição química. Técnicas convencionais de extração como a hidrodestilção, podem resultar em alterações da composição química original destes óleos. O uso da HS-SPME provê uma alternativa mais branda de extração, prevenindo transformações químicas e resultando em informações complementares sobre a composição de voláteis das plantas. Pela primeira vez, cumarina e cumaran foram identificados no "headspace" de folhas de E. laevigatum, após dano mecânico, por HS-SPME. As semelhanças e diferenças na composição dos voláteis, verificadas através de ambas as técnicas de extração, são discutidas, mostrando a complementaridade destas técnicas, as possíveis implicações destes resultados no que diz respeito a compostos infoquímicos e as possíveis transformações químicas durante o processo de hidrodestilação.In this work the qualitative chromatographic profiles of the volatile oil obtained with fresh chopped leaves of Baccharis punctulata, Baccharis dracunculifolia and Eupatorium laevigatum, using HS-SPME were compared with their hydrodistilled oils. Several Brazilian native plant species have not yet been studied regarding their volatile compounds composition. Conventional techniques employed for the investigation of volatile compounds, such as hydrodistillation, may impart chemical changes to the original oil composition. The use of HS-SPME provides alternative milder extraction conditions, preventing chemical transformations and supplying complementary information about volatiles composition. Coumarin and coumaran were detected by the first time among volatile components of E. laevigatum leaves after mechanical damage, only when using HS-SPME. Differences and similarities perceived between volatile compounds profiles using both extraction techniques are discussed, showing that they are complementary and may bring insight about fresh leaf volatiles playing infochemical roles and about chemical transformations caused by hydrodistillation.

Changes in the volatile organic profile of Schinus polygamus (Anacardiaceae) and Baccharis spicata (Asteraceae) induced by galling psyllids

“…This monoterpene was detected in the headspace of the galls of S. polygamus, but was not detected in the headspace of healthy leaves or in the headspace of healthy parts of galled leaves of this plant species. On other hand, β-pinene Parafinic alkane n-heptane (6) (701, 700) n-nonane (15) (900, 900) n-undecane (35) (1097, 1100)…”

“…n-heptane (6) (701,700) n-nonane (15) (900, 900) n-undecane (35) (1097, 1100) b HPGL healthy part of galled leaves; numbers between parenthesis after compound name designates the peak of the compound in Figures 2 and 3; numbers between parenthesis below compound names are: retention index obtained experimentally, retention index from the scientific literature. 26 was detected in the headspace of healthy leaves and galls of B. spicata but showed a three times increase in galled tissues (from 13.5 ± 3.7 to 40.9 ± 4.4).…”

“…13 This genus is well-studied and produces a variety of compounds of different structural types. Terpenoids, 12,[14][15] are mainly reported for its pharmacological and biological activity, being widely used in the perfumery industry and traditional medicine. [17][18][19] Baccharis spicata is a shrub native of South Brazil, Paraguay, Uruguay and Argentina.…”

Characterization and extraction of volatile compounds from pineapple (Ananas comosus L. Merril) processing residues

“…When high-purity chemicals were not available, the comparison was made with retention data and LRI reported in the literature (ADAMS, 2007;KONDJOYAN;BERDAGUÉ, 1996;JENNINGS;SHIBAMOTO, 1980) for tentative identification of the compounds. Comparison between the mass spectra of the National Institute of Standards and Technology (NIST, USA) and that of the unknown compounds spectra was also made for tentative identification (SCHOSSLER et al, 2009).…”

Volatile compounds from Rosmarinus officinalis L. and Baccharis dracunculifolia DC. Growing in southeast coast of Brazil

“…3 Higher yield of nerolidol (22.16%), the main constituent, and spathulenol (8.8%) was also detected from samples collected in Rio Grande do Sul (South Brazil) and isolated through hydrodistillation, totalizing over 30% of leaf volatiles. 12 Nerolidol and spathulenol, part of the oxygenated fraction, obtained from B. dracunculifolia volatile extracts have a high commercial value in perfumery industries. 13 In samples of B. dracunculifolia volatiles from Rio de Janeiro was not detected spathulenol and nerolidol, although these sesquiterpenes are typical in most R. officinalis plants, suggesting that these samples from Southeast coast of Brazil showed lower quality and are not accepted by pharmacopeias.…”