Chemiluminescence determination of mezlocillin by the luminol-potassium periodate system

Abstract

Um método novo, simples e sensível foi proposto para a determinação de mezlocilina. Baseia-se na amplificação da emissão de quimiluminescência (CL) gerada a partir da oxidação do luminol em meio alcalino pelo periodato de potássio. A otimização das variáveis experimentais e instrumentais que afetam o efeito da amplificação de CL foi realizada usando-se sistema de injeção em fluxo. Em condições ótimas, o método é eficiente para determinar mezlocilina no intervalo linear de 0,01 a 30 ×10-6 g mL -1 com limite de detecção (3σ) de 3,0×10 -9 g mL -1 e desvio padrão relativo (RSD) de 1,0% para 1,0×10-6 g mL -1 de mezlocilina (n=11). O método tem sido aplicado com sucesso, para determinação de mezlocilina em preparações comerciais, amostras sintéticas e formulações biológicas fluidas.A new, simple and sensitive method has been proposed for the determination of mezlocillin. It is based on the enhancement of the chemiluminescence (CL) emission generated from the oxidation of luminol in alkaline medium by postassium periodate. The optimization of the experimental and instrumental variables affecting the CL enhancement effect has been carried out using flow-injection system. In the optimum conditions, the method is efficient to determine mezlocillin in the linear range of 0.01-30×10-6 g mL -1 with a detection limit (3σ) of 3.0×10 -9 g mL -1and the relative standard deviation (RSD) is 1.0% for 1.0×10 -6 g mL -1 mezlocillin (n=11). It has been successfully applied to the mezlocillin determination in commercial preparations, synthetic samples and biological fluids formulations.

A novel luminol-based chemiluminescence method for the determination of amikacin sulfate in serum by using trivalent copper-periodate complex

“…The oxidation of luminol (3-aminophthalhydrazide) in alkaline medium is one of the most efficient CL reactions and has been widely used in the quantitative analysis of many inorganic and organic compounds [16] . These luminol-based CL system needs some oxidants such as hydrogen peroxide, oxygen, potassium permanganate, ferricyanide, periodate and oxygen free radicals [17] , [18] , [19] , [20] , [21] , [22] , [23] . Since then, there has been a continuing interest in the development of novel oxidant reagents for the luminol-based CL reaction, which can widen the application of the luminal-based CL reaction.…”

A flow injection chemiluminescence method for the determination of lincomycin in serum using a diperiodato‐cuprate (III)–luminol system

“…It has been widely used in quantitative analysis of many inorganic and organic compounds (21). In the luminol-based CL system, the commonly used oxidants include hydrogen peroxide, oxygen, potassium permanganate, ferricyanide, periodate, persulfate, hemoglobin and myoglobin (22)(23)(24)(25)(26)(27)(28). In recent years, there has been renewed interest in the development of new oxidant reagents for the luminolbased CL reaction, which can widen the application of CL analysis.…”