Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs

Abstract

O planejamento fatorial fracionário e com ponto central foram aplicados no desenvolvimento de um biossensor amperométrico para determinação do vírus da hepatite C. As biomoléculas foram imobilizadas por adsorção em eletrodos de grafite modificados com filme sol-gel preparado com matriz híbrida siloxano-poli(propileno-óxido). Diversos parâmetros foram otimizados, tais como a concentração da estreptavidina em 0,01mg mL -1 e albumina de soro bovino em 1,0%, o tempo de incubação dos eletrodos de 30 minutos na solução do DNA complementar e a diluição do conjugado avidina-peroxidase em 1:1500, entre outros. A aplicação de estudos quimiométricos mostrou-se eficiente, pois as melhores condições foram estabelecidas com um menor número de experimentos, além de apontar quais os fatores exercem maiores influências sobre o sistema.Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL -1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.

HCV Detection, Discrimination, and Genotyping Technologies

“…Uliana et al reported the amperometric biosensor for the detection of HCV using fractional factorial designs [ 87 ]. The biomolecules were immobilized on the graphite electrodes pre-modified with siloxane-poly(propylene oxide) hybrid matrix prepared by following the sol-gel method.…”

Amperometric Biosensor for Diagnosis of Disease

“…Chemometric studies were applied to the development of another biosensor for hepatitis C virus using PGE [87]. Fractional factorial and factorial with center point design were applied in order to simultaneously evaluate the variables of interest that have significant influence on the biosensor response.…”

Analytical chemistry in Brazil: healthy and growing

“…Uliana et al 260 used fractional factorial design for development of an amperometric biosensor for detection of the hepatitis C virus. Santos and Masini used RSM to study four variables for optimization of Cu phthalocyanine photo-Fenton degradation.…”