Determination of metoprolol, acebutolol and propranolol in pharmaceutical formulations using the same SIA system

Abstract

Apresenta-se um sistema mecanizado para a determinação de β-bloqueadores em preparações farmacêuticas. Através da utilização da técnica de Análise de Injeção Sequencial (SIA) foi obtida uma metodologia simples, econômica e versátil, adaptável a todo o tipo de controlo farmacêutico envolvendo estas substâncias. As amostras não necessitam de pré-tratamento devendo apenas ser dissolvidas em ácido antes de serem analisadas. ). O desvio padrão relativo foi inferior a 5 % em todas as determinações. A metodologia foi aplicada em comprimidos, injectáveis e cápsulas de libertação prolongada. Os excipientes que, usualmente, são empregues nas preparações farmacêuticas, não interferem. Os resultados obtidos usando a metodologia proposta foram estatisticamente comparáveis com os obtidos pelos métodos de referência (nível de confiança de 95%). O sistema SIA produz apenas 2,5 mL de efluentes por determinação enquanto que os métodos de referência originam 140 mL.A mechanized system for the determination of β-blockers in pharmaceutical formulations is presented. Using the Sequential Injection Analysis (SIA) technique it was achieved a simple, economical and versatile methodology adaptable to any pharmaceutical control involving these substances. It does not require any pre-treatment for the samples, as they must only be dissolved in acid before analysis. Linear calibration plots were obtained for metoprolol (40.52 -250 mg L ). A R.S.D. lower than 5% was attained. The methodology was used in tablets, injections and prolonged-release capsules. Common excipients used in pharmaceuticals do not interfere. Statistical comparison of the results obtained with the proposed methodology and with the official methods showed good agreement (95% confidence level). SIA system produces only 2.50 mL of effluents per determination whereas the reference methodologies consume around 140 mL.

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

“…The detection limit was better than those of previous reports (from 5.1 to 4.97 mg/L). [1][2][3][4][5][6][7][8][9][10] The relative standard deviations (RSD) of the ECL intensity and the migration time for six consecutive injections of 1.0 μg/mL PRO were 2.6 and 2.1%, respectively.…”

“…Current methods for the detection of PRO include spectrophotometry, FI-CL, AAS, HPLC and LC-MS. [4][5][6][7][8][9][10][11][12][13] However, spectrophotometry was not sensitive for determination of PRO, the use of spectrofluorometry for determining drugs in biological fluids was made difficult by the presence of natural fluorescent interferences, FI-CL had low sensitivity and poor reproducibility, and AAS and HPLC demanded complex extraction procedures and pretreatments such as preconcentration in an organic solvent or proteins precipitation and are not environmentally friendly. In addition, mass spectrometry provides the high recorded mass resolution, high sensitivity and good reproducibility, but it requires access to an expensive mass spectrometer.…”

Detection of Metoprolol in Human Biofluids and Pharmaceuticals via Ion-Transfer Voltammetry at the Nanoscopic Liquid/Liquid Interface Array

“…So far, numerous methods have been developed for the determination of MTP in different media, such as pharmaceutical preparations, human urine, serum, plasma, and blood. − Among them, chromatography is one of the core technologies, in conjunction with using different detection techniques, such as mass spectrometry (MS), ,, ultraviolet spectrophotometry (UV), ,, and fluorescence . However, these techniques are usually expensive and require time-consuming preseparation or pretreatments.…”