Determination of losartan associated with chlorthalidone or hydrochlorothiazide in capsules by capillary zone electrophoresis

Abstract

Uma metodologia alternativa por eletroforese capilar de zona (ECZ) é proposta para a análise de losartan potássio (LOS) associado com clortalidona (CTD) ou hidroclorotiazida (HCT) em cápsulas, usando 50 mmol L -1 de tampão carbonato de sódio com detecção em 226 nm. O sistema de eletrólito foi otimizado, levando em consideração o perfil do pico, resolução, tempo de análise, estabilidade da linha base e repetibilidade (ausência de adsorção). O método foi aplicado com sucesso na análise de formulações farmacêuticas adquiridas em farmácias de manipulação. Os analitos apresentaram boa separação na linha base em menos de 5 min. Os resultados obtidos não apresentaram diferença signicativa no intervalo de 95% de confiança em relação ao valor de referência. O método pode ser aplicado para a análise destes compostos ou empregado em outros formulações framacêuticas similares contendo estas drogas.An alternative methodology by capillary zone electrophoresis (CZE) is proposed for the determination of losartan potassium (LOS) associated with chlorthalidone (CTD) or hydrochlorothiazide (HCT) in capsules, using 50 mmol L -1 of sodium carbonate buffer with detection at 226 nm. The electrolyte system was optimized, taking into account peak profiles, resolution, run times, baseline stability and repeatability (adsorption absence). The method was successfully applied to the analysis of pharmaceutical formulations purchased in manipulation drugstores. The analytes were baseline separated in less than 5 min. The results did not present significant difference in the 95% confidence interval in relation to the label claim values. The method can be applied to analyze these compounds and could also be employed in other similar formulated products containing these drugs.

Simultaneous determination of atenolol and amiloride in pharmaceutical preparations by capillary zone electrophoresis with capacitively coupled contactless conductivity detection

“…The simultaneous determination of AT and CD in formulations using CZE method was also reported (Al Azzam et al, 2009). Balesteros et al (2007) developed a CZE method for the determination of CD and losartan in capsules. Reports for determining CD by gas chromatography (GC) (Magnar and Klas, 1974;Degen and Schweizer, 1977) or liquid chromatography (LC) for the simultaneous determination of both AT and CD (Dadgar and Kelly, 1988;Muirhead and Christie, 1987;Giachetti et al, 1997;Rapado-Martinez et al, 1997;El-Gindy et al, 2008;Sa'sa et al, 1988) can also be found, or for the simultaneous determination of β-blocker drugs (AT, pindolol, metoprolol and labetolol) using HPLC coupled with a capacitively coupled contactless conductivity detector (HPLC-C 4 D) (Kubáň et al, 2006).…”

“…The fi rst is a pressure regulator, while the second keeps liquid retention under control. By combining these two pharmaceuticals with diff erent action mechanisms, it is possible to use low doses and to minimize their side eff ects (Balesteros et al, 2007). Chlorthalidone (CD) is a diuretic with actions and indications similar to those of the thiazide diuretics.…”

Voltammetric Determination of Anti-Hypertensive Drug Hydrochlorothiazide Using Screen-Printed Electrodes Modified with L-Glutamic Acid

“…Prominent among them is the high performance liquid chromatography (HPLC) with UV-VIS detection [3][4][5], which is the analytical technique recommended by the United States Pharmacopeia [6]. Although HPLC/tandem MS [7] or capillary electrophoresis [8,9] are also considered. Methods for the individual determination of HCTZ are also described.…”

Simultaneous determination of atenolol, chlorthalidone and amiloride in pharmaceutical preparations by capillary zone electrophoresis with ultraviolet detection

“…Moreover, a CZE method for the simultaneous determination of AT and CD in formulations was recently reported (Al Azzam et al, 2009). Balesteros et al, (2007) developed a CZE method for the determination of CD and losartan in capsules. Reports for determining CD by gas chromatography (GC) (Magnar and Klas, 1974;Degen and Schweizer, 1977) can also be found.…”