Abietane diterpenes from Sagittaria montevidensis ssp montevidensis Charm. & Schltdl

Abstract

Dois novos diterpenos do tipo abietano 3β-hidroxi-9α, 13α-epidioxiabiet-8(14)-eno (1) e 3-oxo-9α,13α-epidioxiabiet-8(14)-eno (2) foram isolados do extrato bruto metanólico dos pecíolos de Sagittaria montevidensis ssp montevidensis (Alismataceae). As estruturas de 1 e 2 foram determinadas com base em análises espectrométricas do tipo EM-AR, IV, bem como, RMN de 1 H e 13 C uni e bi dimensionais.Two new abietane-type diterpenes, 3β-hydroxy-9α, 13α-epidioxyabiet-8(14)-ene (1) and 3-oxo-9α,13α-epidioxyabiet-8(14)-ene (2), were isolated from the methanolic crude extract of the petioles of Sagittaria montevidensis ssp montevidensis (Alismataceae). The structures of 1 and 2 were determined on the basis of spectrometric analyses including HREIMS, IR as well as 1 H and 13 C 1 and 2D NMR.

LC-MS- and 1H NMR Spectroscopy-Guided Identification of Antifungal Diterpenoids from Sagittaria latifolia

“…This genus consists of nearly 25 species distributed through the tropical regions of North and South America . Previous phytochemical investigations of other Sagittaria species revealed the presence of various chemotypes of diterpenoids. − Several ent -rosane and ent -kaurane diterpenoids showed potent antimicrobial activities against oral pathogens. , To identify the antifungal compound(s), a scale-up extraction of the flowers and leaves of S. latifolia , followed by isolation and structure elucidation of bioactive components, was conducted, leading to the identification of two new isopimaradienes ( 1 and 2 ). Using this as an example, the study reported here further illustrates the advantage of the combined LC-MS and 1 H NMR spectroscopic analytical tools for hit prioritization in natural products drug discovery.…”