A simple colorimetric method for the determination of carbofuran and its application in environmental and biological samples

Tamrakar, Urmila; Pillai, Ajai K.; Gupta, V. K.

doi:10.1590/s0103-50532007000200014

Cited by 13 publications

(10 citation statements)

References 4 publications

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“…Briefl y, urine samples ( The limit of detection (LOD) and the limit of quantifi cation (LOQ) were calculated as the concentration of TCP with signal-to-noise (S/N) ratios of about 3/1 and 10/1, respectively and have the following values: LOD = 0.001 µg/mL and LOQ = 0.005 µg/mL. From the group of carbamate pesticides, carbofurane (2,3-dihydro,2,2-dimethyl-7-benzofuranyl carbamate) was analyzed in the biological samples by its reaction with diazotized p-aminoacetophenone under alkaline condition, when the orange dye formed was measured at 460 nm [25,26].…”

Section: Methodsmentioning

confidence: 99%

Multiple pesticides exposure of greenhouse workers and thyroid parameters

Simescu¹,

Igna²,

Nicolaescu³

et al. 2014

Int. J. SDP

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Objective: The persistence of high prevalence of thyroid pathology (goiter, nodules) in Romanian population, despite the correction of iodine defi ciency, determined us to evaluate the impact of factors different than iodine intake on the thyroid gland as environmental endocrine disruptors. We studied the potential correlation between pesticide exposure and parameters of the function, autoimmunity and morphology of the thyroid in a group of greenhouse workers (GHWs) exposed to multiple uncontrolled pesticides across agricultural seasons. Materials and methods: A group of 108 GHWs, 18-78 year olds, with normal iodine intake, from a plain village, exposed to multiple pesticides was enrolled voluntary in this study. Thyroid echography, thyroid parameters [thyroid stimulating hormone (TSH), free T4 (FT4), and antibody to thyroid peroxidase (ATPO)], some of the used pesticides (chlorpyrifos, trichloropyridinol (TCP), carbofurane, cypermethrin, dimethoate) and cholinesterase activity were determined in biological samples (urine, blood) collected across two agricultural seasons. Results: The median urinary iodine concentration in children from this village was 135.20 µg/L, while median TSH and median FT4 was 1.72 μUI/mL and 16.68 pmol/L, respectively. Hypothyroidism (TSH > 4.2 μUI/mL) was present in 12.4% of patients, median TSH value was 7.26 μUI/mL. In four of them post-thyroidectomy hypothyroidism was present. Elevated titres of anti-TPO antibodies were found in 22 (20.37%). Echographic pattern of thyroiditis was present in 16.49% of the patients, and thyroid nodules were detected by ultrasonography in 38.13%. There were two subjects with Graves' disease. The detectable level of TCP was bigger in the fi rst season (range 120-190 μg/L) than in the second (range 0.7-1.7 μg/L). The range of seasonal concentration of urinary carbofurane was 0.004-0.25 μg/mL. Cypermethrin was detected in a small number of subjects (range: 12.5-13.3 μg/g creatinine). Dimethoate was undetectable. Conclusions: The distribution of thyroid disorders in study group living in an area with normal iodine intake did not differ from known epidemiological studies. The most frequently encountered pesticide was chlorpyrifos. Some samples presented several pesticides simultaneously.

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Section: Methodsmentioning

confidence: 99%

Multiple pesticides exposure of greenhouse workers and thyroid parameters

Simescu¹,

Igna²,

Nicolaescu³

et al. 2014

Int. J. SDP

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“…Dentro de los pesticidas más utilizados en Colombia está el carbofurano (2,3 dihidro-2,2-dimetil-7-benzofuranil-metilcarbamato), que es un insecticida y acaricida de contacto, sistémico y de amplio espectro, usado en una gran variedad de cultivos (Tamrakar et al 2007;Bermúdez et al 2011). Su alta toxicidad y acumulación en los seres vivos ha incrementado la preocupación en cuanto a la salud humana y la seguridad alimentaria (Garbin et al 2007;Khanmohammadia et al 2007;Wang et al 2009).…”

Section: Introductionunclassified

“…También se reporta que es teratogénico, genotóxico, mutagénico y afecta a los embriones (Cohen et al 1994;Gera et al 2011). Debido a su gran toxicidad y aplicabilidad se reportan varios métodos de cuantificación de sus residuos, dentro de los que están la espectrofotometría UV-Vis (Tamrakar et al 2007), la cromatografía líquida de alto rendimiento (Tennakoon et al 2009;Otieno et al 2010;Bermúdez et al 2011), la cromatografía de gases (Petropoulou et al 2006), la fluorimetría (Pacioni y Veglia 2003), los sensores amperométricos (Sun et al 2012) y la cromatografía de gases acoplada a espectrometría de masas (Bravo et al 2005;Tennakoon et al 2009;Otieno et al 2010). …”

Section: Introductionunclassified

Cuantificación voltamétrica de carbofurano en papa (Solanum tuberosum L., Solanaceae)

Lara-Sandoval

Acuña

2017

Corpoica Cienc. Tecnol. Agropecuaria

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Sanidad vegetal y protección de cultivosCuantificación voltamétrica de carbofurano en papa (Solanum tuberosum L., Solanaceae)Voltammetric quantification of Carbofuran in potato (Solanum tuberosum L., Solanaceae)Quantificação voltamétrica de carbofurano em batata (Solanum tuberosum L., Solanaceae) Artículo de investigación científica y tecnológicaAdriana Elizabeth Lara-Sandoval,1 Sandra Patricia Chaparro-Acuña2 ResumenEl exceso en la aplicación de plaguicidas puede generar acumulaciones en los tubérculos de papa que sobrepasen el límite máximo residual (LMR) presentado por el Codex Alimentarius, lo cual representa riesgos para la salud de los consumidores. La cuantificación de plaguicidas es un proceso costoso y de difícil acceso para los cultivadores y entes de salud y de vigilancia. En este artículo se presenta una metodología sencilla, económica y asequible para la cuantificación de carbofurano en tubérculos. El método de voltametría diferencial de pulso (DPV) para carbofurano fue validado para su cuantificación en papa. El estudio fue realizado usando como electrolito de soporte la solución bufferPalabras clave: electroquímica, insecticidas, productos agrícolas, Solanum tuberosum Britton-Robinson, un electrodo de trabajo de carbón vítreo, Ag/AgCl como electrodo de referencia y platino como electrodo auxiliar. El insecticida en papa fue extraído por reparto sólido-líquido y los resultados obtenidos fueron contrastados por espectroscopía UV-Vis, lo cual permitió deducir que el método voltamétrico genera resultados satisfactorios, precisos y confiables para la cuantificación de carbofurano en muestras de este tubérculo. Estos resultados son una evidencia del uso de carbofurano en el municipio de Ventaquemada (Boyacá). Este método de detección de carbofurano puede utilizarse como una herramienta para monitorear el uso de estos compuestos químicos en la papa. 275-284

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“…Compared with chromatographic methods, the spectrophotometric determination of N-methyl carbamates was a simple, sensitive, much less expensive and sensitive to parent compounds as well as metabolites. 9…”

Section: Introductionmentioning

confidence: 99%

Urine Carbamate Determination Compared with Plasma Pseudocholinesterase for Diagnosis of Insecticides Poisoning

Masry¹

2016

MOJT

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Introduction: Diagnosis of carbamate poisoning is frequently missed especially when patients present after the recovery of pseudocholinesterase inhibition. Confusion is due to the similarity of the clinical picture with that of food poisoning and other infectious syndromes. Patients and methods: Patients (n=40) admitted within 4 hours of carbamate intoxication were included in the study. They were graded according to Poison severity score PSS. Normal volunteers (n=10) were included and served as negative control group. Normal subjects and all patients were tested for plasma pseudocholinesterase (PsChE) within 4 hours and 18 hours from carbamates exposure. Urine carbamate concentration was determined on the 18 th hour and 2 days after exposure. Different PSS groups and normal volunteers were compared for their plasma PsChE and urine carbamate levels using student t test and correlation coefficient Method used for PsChE determination is the kinetic assay method. Urine carbamate concentrations were determined by spectrophotometry using modification of the method of Tamrakar. Carbamate determination method was tested for linearity, precision, recovery and limit of detection of the carbamates Results: PsChE was significantly lower in PSS II compared to PSS I. No significant difference in PsChE was noticed between both patient's groups and normal volunteers on the 18 th hour from carbamate exposure. Urine carbamate concentration was significantly higher in PSS II compared to PSS I on the 18 th hour and 2 days after exposure. No correlation was detected between the maximum urine carbamate concentration and plasma pseudocholinesterase. The method specificity increased after modification from 76.9 to 91%. Recovery ranged between 95 and 98%. Limit of detection is 0.1µg/ml and limit of quantification is 0.2µg/ml. The technique is highly reproducible with inter-day precision RSD less than 8%. Conclusion: A better evaluation and diagnosis of carbamate poisoning patients with delayed presentation can be achieved using urine carbamate determination rather than plasma pseudocholinesterase which is often normalized at the time of presentation

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A simple colorimetric method for the determination of carbofuran and its application in environmental and biological samples

Cited by 13 publications

References 4 publications

Multiple pesticides exposure of greenhouse workers and thyroid parameters

Multiple pesticides exposure of greenhouse workers and thyroid parameters

Cuantificación voltamétrica de carbofurano en papa (Solanum tuberosum L., Solanaceae)

Urine Carbamate Determination Compared with Plasma Pseudocholinesterase for Diagnosis of Insecticides Poisoning

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