The use of water soluble tertiary amine reagent for solubilization and metal determination in fish muscle tissue

Abstract

A extração de metais com aminas terciárias solúveis (reagente CFA-C) assistida por ultra-som e em presença de peróxido de hidrogênio foi usada para solubilização de metais em amostras de peixe. Duas espécies de peixes de águas naturais (Pseudoplatystoma coruscans e Salminus brasiliensis), diferindo basicamente no teor de lipídio, foram utilizadas. A etapa de otimização foi desenvolvida com o auxílio de planejamento fatorial. O procedimento proposto permitiu a extração quantitativa de Ca, Cu, Fe, Mg e Zn. No processo de validação da metodologia analítica, a exatidão foi comparada com procedimento de digestão ácida em bombas Parr e usando um material certificado de referência (Fish homogenate, MA-A-2, IAEA). Todos os resultados obtidos foram concordantes ao nível de 95% de confiança, em teste t-pareado.The ultrasound assisted metal extraction with water soluble tertiary amines (CFA-C reagent) in presence of hydrogen peroxide was used for improving metal solubilization in fish samples. Two species of freshwater fish (Pseudoplatystoma coruscans e Salminus brasiliensis), differing basically in lipid content were used. The optimization step was carried out using a factorial experimental design. The proposed procedure allowed the quantitative extraction of Ca, Cu, Fe, Mg, and Zn. In the validation process accuracy was evaluated by comparison with the total acid digestion of the sample in Parr a digestor and using a certified reference material (Fish homogenate, MA-A-2, IAEA). All results were in agreement at a 95% confidence level according to a paired-t test.

Flow injection preconcentration system using a new functionalized resin for determination of cadmium and nickel in tobacco samples

“…Samples were digested by adding 4.0 mL of 1:1 (v/v) nitric acid solution on sample. The acid digestion bomb was heated in a stove at 150 • C for 5 h [24]. Blank samples were prepared in the same way.…”

Preconcentration system for cadmium and lead determination in environmental samples using polyurethane foam/Me-BTANC

“…Real samples of black tea and spinach leaves were also analysed. These materials were digested using the following procedure: a portion of 0.2 g of dry sample was precisely weighted into a Teflon cup, 4.0 ml of 1:1 (v/v) nitric acid solution were added, and the acid digestion bomb was heated in a stove at 150 • C for 5 h [29]. After cooling at room temperature the bomb was opened carefully in a fume cupboard.…”

Optimization of microwave assisted digestion procedure for the determination of zinc, copper and nickel in tea samples employing flame atomic absorption spectrometry

“…These design structures are based on full two-level factorial design by center point replication and inclusion of an axial portion. Several digestion procedures for natural samples have been optimized using this chemometric tool [17][18][19][20][21].…”