Infraspecific variation in the chemistry of glandular trichomes of two Brazilian Viguiera species (Heliantheae; Asteraceae)

Abstract

Através do emprego da técnica de microamostragem de tricomas glandulares e análises por CLAE em sistema isocrático, foi efetuada a investigação química dos tricomas glandulares de diferentes populações de duas espécies de Viguiera. Nas seis amostras de V. robusta analisadas, o furanoeliangolido budleína A e seus isômeros contendo tiglato e metacrilato foram detectados como constituintes majoritários. Também foram detectados vários outros constituintes, na maioria em quantidades bem menores ou apenas traços. O perfil químico de todas as amostras foi qualitativamente muito similar, caracterizando V. robusta como um táxon com elevada "quimioconsistência". Por outro lado, V. quinqueremis representou um exemplo de "quimiodiversidade". Embora a budleína A e seus derivados também estivessem presentes em cinco das seis populações analisadas, outros heliangolidos e germacranolidos também ocorreram e dominaram parcialmente em quantidade. Padrões distintos destas substâncias dividiram as amostras de V. quinqueremis em três subgrupos químicos.A chemical survey on the glandular trichome chemistry of different populations of two Brazilian Viguiera species has been performed by the glandular trichome microsampling technique and isocratic HPLC analyses. In all six analysed samples of V. robusta, the furanoheliangolide budlein A and its tiglate and methacrylate isomers were detected as the major compounds. They were accompanied by various constituents in mostly minor or trace amounts. The chemical pattern of all samples was qualitatively very similar, thus featuring V. robusta as a taxon of high "chemoconsistency". In contrast, V. quinqueremis represented an example of "chemodiversity". Although budlein A and its derivatives were present in five of the six analysed populations, other heliangolides and germacrolides co-occurred and partly dominated in quantity. Distinct compound patterns divided the samples of V. quinqueremis into three chemical subgroups.

Anatomical studies of the secretory structures: Glandular trichomes and ducts, in Grindelia pulchella Dunal (Astereae, Asteraceae)

“…(Pagni and Masini, 1999;Pagni et al, 2003), Stevia Cav. (Cornara et al, 2001), Tagetes L. (Poli et al, 1995), and Viguiera Kunth (Da Costa et al, 2001).…”

Further Sesquiterpene Lactones from Viguiera robusta and the Potential Anti-Inflammatory Activity of a Heliangolide: Inhibition of Human Neutrophil Elastase Release

“…In a previous work (Da Costa et al, 1996), the STLs budlein A tiglate and atripliciolide tiglate were isolated from the crude extract of the leaves of V. robusta Gardn. Later, budlein A and its analogues were detected by HPLC in the glandular trichomes of the leaves of different populations of V. robusta (Da Costa et al, 2001). In this work, besides budlein A tiglate, we describe the isolation of large amounts of budlein A as well as the heliangolide niveusin A and a new eudesmanolide from the leaf rinse extract of V. robusta.…”

Seasonal variation of the essential oil from two Brazilian native Aldama La Llave (Asteraceae) species

“…In A. robusta, the diterpenes manoyl oxide and manool, which belong to the labdanes class, were identified in the present study. Previous studies have identified heliangolides (Da Costa et al 2001) and diterpenes derived from kauranes (Da Costa et al 1996) in Aldama robusta (=Viguiera robusta).…”