HPLC analysis of flavonoids in Eupatorium littorale

Oliveira, Brás Heleno de; Nakashima, Tomoe; Filho, José Dias de Souza; Frehse, Fabiano L.

doi:10.1590/s0103-50532001000200019

Cited by 26 publications

(14 citation statements)

References 13 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reversed-phase HPLC is one of the most employed techniques for the analysis of flavonoids [15]. Some results using HPLC were reported for Alpinia officinarum and A. purpurata species [16,17].…”

Section: Hplc Analysismentioning

confidence: 99%

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

2009

View full text Add to dashboard Cite

Abstract:The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversedphase HPLC techniques the rutin and kaempferol-3-O-glucuronide were detected. Ethanol 70% was more efficient for flavonoids extraction than water. No significant yielding variation was verified for ultrasonic, microwave and stirring methods using ethanol 70% (11 to 14%). The relative concentration of rutin and kaempferol-3-O-glucuronide, respectively, was higher by ultrasonic (1.5 and 5.62 mg g -1 dried leaves, respectively) and by microwave (1.0 and 6.64 mg g -1 dried leaves) methods using ethanol. Rapid and simplified extraction proceeding optimize phytochemical work and acquisition of secondary metabolites.Keywords: high performance liquid chromatography; maceration; microwave; ultrasonic; Zingiberaceae.Rio de Janeiro. Voucher specimen was identified and deposited in the Herbarium of Rio de Janeiro Botanical Garden, accession number RB 433485.All chemicals used in analysis as methanol and phosphoric acid were of HPLC grades and were purchased from Merck. MilliQ water was utilized to HPLC mobile phase and sample preparation. Kaempferol-3-O-glucuronide was isolated from Alpinia zerumbet and identified by Nuclear Magnetic Resonance (NMR) [3]. Rutin was purchased from Merck. Preparation of extractsLeaves of A. zerumbet were collected from adult plants, in the morning, then plant material was dried for 3 days in stove (60 o C) and macerated in 70% ethanol or distillated water, in the same proportion of 1 g dried leaves/20 mL solvent (10% w/v).It was evaluated four extraction methods from dried leaves: maceration in shaker at 100 rpm, ultrasonic bath (40 kHz, Thornton Unique, model 1400 USC), microwave (PANASONIC -Auto Sensor Diet, full power) and stirring (Table 1). In the microwave extraction, the suspensions were irradiated under microwaves in pre-setting procedures (3 s power on, 60 s off) for three times to the desired temperature about 60 and 70 o C. The temperature was measured after turn off the microwave using a thermometer into extracts in Becker. For the ultrasonic extraction, the 30 mL flask containing 1 g of dried leaves plus 20 mL of one of extracting solvents was partially immersed into the ultrasonic bath and temperature was controlled.Preliminary results obtained by different extraction time 15 and 30 min for ultrasonic and 1 and 2 days for maceration techniques did not indicate significant differences in relation to extraction time presented in this study.Crude extracts were filtered in vacuum using Whattman ® filter (110 mm ø, 1). Aqueous extracts were frozen, then liophylizated and the hydroalcoholic extracts were evaporated to dry under reduced pressure at 60 o C. The dried weight was measured. The yielding was defined as follows: (crude extract weight/plant material weight) x 100....

show abstract

Section: Hplc Analysismentioning

confidence: 99%

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

2009

View full text Add to dashboard Cite

show abstract

“…The presence of various bioactive compounds such as vanillic acid, hydroxy benzoic acid, Gallic acid, Coumaric acid, syringic acid, caffeic acid and cinnamic acid, and flavonoids including quercetin, vitexin, rutin, luteolin and hispidulin, essential oils, terpenoids, sugars, aromatic aldehydes justifies the use of the plant for various ailments by traditional practitioners. The analysis time is an important factor in analytical work and the run time should be reduced to a minimum to optimize equipment use and reduce solvent consumption [25].…”

Section: Resultsmentioning

confidence: 99%

ACTIVE PHENOLIC CONSTITUENTS AND BRINE SHRIMP LETHALITY ASSAY OF HYDROALCOHOLIC EXTRACT OF Plectranthus amboinicus

Janakiraman

Somasundaram²

2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: Plectranthus amboinicus (PA) leaves contain pharmaceutically important phytoconstituents that attribute to greater medicinal values. The purpose of this study was to investigate the active principles present in the potent hydroalcoholic extract of PA (HAPA) and brine shrimp lethality studies to understand the toxicity profile of PA, to unravel many potential applications in the field of science.Methods: An approach toward high performance liquid chromatography (HPLC) screening of (HAPA) and selective partitioning using various solvents such as hexane, chloroform, ethyl acetate, and butanol were performed to identify the active principles present. Acute oral toxicity or brine shrimp lethality studies on Artemia salina L. using active fraction of PA were also attempted. Results:The HPLC profile of ethyl acetate fraction of hydroalcoholic extract showed flavonoids such as Rutin, quercetin, luteolin and gallic acid, which was found to be considerably different from hydroalcoholic extract and this fraction also showed good acute oral toxicity effect and the LC50 value obtained was 198.630 µg/ml. Conclusion:These results attribute to the pharmacological importance of the plant. Selective isolation of individual phytochemical constituents and subjecting it to biological testing will definitely give promising results for the medicinal field.

show abstract

“…Eupafolin and hispidulin were extracted from leaves of Eupatorium litoralle Cabrera from the Asteraceae family and their structures confirmed as described in Oliveira et al (2001). The aerial parts were collected in Piraquara (Metropolitan Region of Curitiba-Brazil) and identified by Prof. Olavo Guimarães, the curator of UFPR Herbarium, where a voucher specimen has been deposited (# UPCB12400) .…”

Section: Flavonesmentioning

confidence: 99%

Effects of natural flavones on membrane properties and citotoxicity of HeLa cells

Herrerias¹,

Oliveira²,

Belem³

et al. 2010

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO: "Efeitos de flavonas naturais em propriedades de membranas e em citotoxicidade de células HeLa". O objetivo deste estudo foi avaliar se eupafolina e hispidulina, flavonas extraídas do Eupatorium littorale Cabrera, Asteraceae, possuíam a capacidade de alterar propriedades das membranas biológicas e promover efeitos citotóxicos. Eupafolina (50-200 µM) reduziu em aproximadamente 30% a velocidade e amplitude do inchamento mitocondrial induzido por valinomicina e 60-100% o inchamento mitocondrial dependente de substrato. Além disso, eupafolina na dose de 200 µM reduziu a transição de permeabilidade mitocondrial em 35% entretanto, a hispidulina não alterou este parâmetro em todas as doses testadas. A avaliação da transição de fase dos lipossomas de DMPC com a sonda DPH demonstrou que ambas as flavonas afetam a fase gel e fluida. Quando lipossomas de membranas mitocondriais e a sonda DPH-PA foram utilizados, houve aumento da polarização de fluorescência promovido pela hispidulina. Eupafolina e hispidulina, na dose de 100 µM, promoveram 40% de redução da viabilidade de células HeLa em 24 h. Nossos resultados sugerem que eupafolina e hispidulina têm efeitos citotóxicos que podem ser explicados em parte pelas alterações promovidas por estas flavonas sobre propriedades de membranas biológicas e sobre a bioenergética mitocondrial.Unitermos: Eupafolina, hispidulina, células HeLa, inchamento mitocondrial. ABSTRACT:The aim of this study was to determine whether eupafolin and hispidulin, flavones extracted from Eupatorium littorale Cabrera, Asteraceae, have the ability to change properties of biological membranes and promote cytotoxic effects. Eupafolin (50-200 µM) decreased approximately 30% the rate and total amplitude of valinomycin induced swelling and 60-100% the energy-dependent mitochondrial swelling. Moreover, eupafolin (200 µM) reduced 35% the mitochondrial permeability transition, and hispidulin did not change this parameter in any of the doses tested. The evaluation of phase transition of DMPC liposomes with the probe DPH demonstrated that hispidulin and eupafolin affect gel and fluid phase. With mitochondrial membrane as model, hispidulin increased the polarization of fluorescence when used DPH-PA probe. Eupafolin and hispidulin (100 µM) promoted a reduction of 40% in cellular viability of HeLa cells in 24 h. Our results suggest that eupafolin and hispidulin have cytotoxic effects that can be explained, in part, by alterations promoted on biological membranes properties and mitochondrial bioenergetics.

show abstract

HPLC analysis of flavonoids in Eupatorium littorale

Cited by 26 publications

References 13 publications

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

ACTIVE PHENOLIC CONSTITUENTS AND BRINE SHRIMP LETHALITY ASSAY OF HYDROALCOHOLIC EXTRACT OF Plectranthus amboinicus

Effects of natural flavones on membrane properties and citotoxicity of HeLa cells

Contact Info

Product

Resources

About