Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic rats

Neto, Sylvio Valença de Lemos; Castiglia, Yara Marcondes Machado; Golim, Márjorie de Assis; Souza, Aparecida Vitória Gonçalves de; Carvalho, Lídia Raquel de; Deffune, Elenice; Nascimento, Paulo do; Módolo, Norma Sueli Pinheiro; Vianna, Pedro Thadeu Galvão

doi:10.1590/s0102-865020170030000004

Cited by 4 publications

(1 citation statement)

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“…Apoptosis is one of the types of cell death and may be triggered by factors such as ischemia, exogenous toxins, and endogenous cytokines 17 , 18 . A growing body of evidence suggests that apoptosis of renal tubular epithelial cells play an important role in sepsis-induced AKI, and apoptosis is one of the major causes of renal damage in sepsis 19 .…”

Section: Discussionmentioning

confidence: 99%

Milk fat globule-epidermal growth factor 8 (MFG-E8) attenuates sepsis-induced acute kidney injury by inhibiting NF-κB signaling pathway

2019

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Purpose To explore the effect of milk fat globule-epidermal growth factor 8 (MFG-E8) on sepsis-induced acute kidney injury (SAKI). Methods Male C57BL/6 mice were randomized to control, sham, CLP, CLP+PBS, and CLP+rmMFG-E8 groups. SAKI was induced by cecal ligation and puncture (CLP). Recombinant mouse MFG-E8 (rmMFG-E8) (20 μg/kg) or PBS (vehicle) was administered intraperitoneally. Blood, urine and renal tissue were collected at 24 h after CLP. Blood samples were tested for serum kidney injury biomarker and cytokines. Urine samples were collected to detect KIM-1, and NGAL. Real-time PCR was tested for Bax and Bcl-2. TUNEL staining was used to determine renal apoptosis. Western blot was used to detect the expression of Bax, Bcl-2, and proteins in the NF-κB pathway. Results MFG-E8 alleviated SAKI by decreasing serum Cre, BUN, urine KIM-1 and NGAL and by mitigating renal pathological changes significant (p < 0.05). IL-1β, IL-6, TNF-α were significantly inhibited by MFG-E8 (p < 0.05). Apoptosis induced by SAKI was markedly suppressed by MFG-E8. Finally, MFG-E8 attenuated the activation of the NF-𝜅B signaling pathway in SAKI. Conclusion MFG-E8 has beneficial effects on SAKI, which may be achieved by inhibiting the NF-κB pathway.

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Section: Discussionmentioning

confidence: 99%