Characterization of an experimental model of progressive renal disease in rats

Baracho, Nilo César do Vale; Kangussu, Lucas M.; Prestes, Thiago Ruiz Rodrigues; Silveira, Kátia D.; Pereira, Regina Maria; Rocha, Natália Pessoa; Silva, Ana Cristina Simões e

doi:10.1590/s0102-865020160110000007

Cited by 9 publications

(6 citation statements)

References 27 publications

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“…This was also confirmed by histopathology and immunohistochemistry of α-SMA in which moderate glomerular hypertrophy with interstitial fibrosis was observed. Similar to the current study, hypertrophy of remnant glomeruli, mesangial expansion, increased glomerular collagen deposition in mice with 5/6 nephrectomy (Gava et al 2012), and tubulointerstitial changes in rats with subtotal nephrectomy were observed (Devada et al 2012 andBaracho et al 2016). In dogs, CKD induction resulted in stable chronic renal failure until 9-12 months (Vaneerdeweg et al 1992), high serum creatinine concentration which was 4.3±1.5mg/dL (Steiner et al 2010), mesangial hyperplasia, and focal glomerular sclerosis (Bourgoignie et al 1987).…”

Section: Discussionsupporting

confidence: 87%

“…Mild injury of the right kidney was confirmed by abdominal ultrasonography, histopathology, weak expression of α-SMA marker in the interstitial tissue and low expression of KIM-1 gene and NGAL gene which are sensitive and specific biomarkers of kidney injury (Rees and Kain 2008;Yin and Wang 2016). Similarly, a previous study on rats showed that the degree of impairment was directly related to the severity of the renal excision (Baracho et al 2016).…”

Section: Discussionmentioning

confidence: 76%

“…Model 1: About one-third of the right kidney mass was removed from the superior portion towards the middle of the pylorus as a modified technique according to Baracho et al (2016) in rats (Fig. 1).…”

Section: Partial Nephrectomy Of the Right Kidneymentioning

confidence: 99%

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Development of Canine Chronic Kidney Disease Model: A Pilot Study

2021

Int J Vet Sci

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Chronic kidney disease (CKD) is a worldwide health problem with a poor prognosis. The exploration of possible medical intervention requires the presence of a suitable animal model. This work aims to induce a model of canine CKD with persistent uremia. Three dogs were used to develop canine CKD models with different techniques based on the remnant kidney model. Model (1) was subjected to excision of one renal pole of the right kidney. Model (2) was subjected to the excision of two renal poles of the right kidney. Model (3) underwent ligation of two renal arterial branches of the right kidney. A contralateral nephrectomy was performed in the three models after two weeks from the first operation. Blood and 24-hour urine samples were collected weekly to determine renal function parameters. Histopathology, Immunohistochemistry of α-SMA expression, and quantitative real-time PCR for Kim-1 & NGAL gene expression were performed on kidney tissues. According to the IRIS staging system in dogs, model (1) represented stage one of CKD with less expression of genes and α-SMA in the interstitial tissue. Model (2) represented stage three of CKD with moderate expression of genes and α-SMA and moderate glomerulointerstitial nephritis. Model (3) suffered from severe uremia. It showed the highest expression of genes and α-SMA with severe glomerulointerstitial nephritis and infarction. Therefore, model (2) was considered the best technique for developing a model of canine CKD with persistent uremia. The dog in this model became uremic and remained stable for a long time which would allow experimental manipulation.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 76%

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Development of Canine Chronic Kidney Disease Model: A Pilot Study

2021

Int J Vet Sci

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“…Additional in vivo models of differing degrees of uraemia can be achieved through the use of an adenine rich diet [43] or a varied surgical approach as described by Baracho et al [44]. At the time of designing our experiments, there were no studies which examined a shorter duration of uraemia, reflecting earlier stage kidney disease, where the response of the vasculature will be different to that of more advanced kidney disease.…”

Section: Discussionmentioning

confidence: 99%

Ergocalciferol improves endothelial vasodilatory and vasoconstrictor function in an in vivo model of mild uraemia

et al. 2019

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Endothelial dysfunction and vitamin D deficiency are prevalent in patients with cardiovascular disease (CVD) and chronic kidney disease (CKD). Both are risk factors for cardiovascular events in patients with CKD. No studies have investigated the effect of nutritional forms of vitamin D on endothelial function in earlier stages of CKD, when vascular endothelium may be more amenable to this therapy. We studied the effect of ergocalciferol in a pre-clinical model of mild uraemia.Male Wistar rats underwent either a 5/6th nephrectomy or sham surgery. Four weeks after the final stage of the surgery, these two groups were randomly allocated to placebo or an oral dose of 1000 iu of ergocalcfierol at day 7 and 2 pre sacrifice. Vascular responses to acetylcholine, Spermine NONOate and phenylephrine were determined in aortic rings. Blood pressure, calcium, phosphate and parathyroid hormone were measured in all groups.Ergocalciferol significantly improved the endothelium-dependent responses to acetylcholine and overcame the blunting of the contractile response to phenylephrine seen in uraemic animals. Ergocalciferol improved the contractile response to potassium chloride in uraemic, but not sham animals. All effects occurred independently of changes to calcium, phosphate, parathyroid hormone and systolic blood pressure. There were no differences in endothelium-independent relaxation to Spermine NONOate.In summary, in a model of mild uraemia, ergocalciferol improved vasodilator and vasoconstrictor tone independently of blood pressure and bone mineral parameters suggesting a direct effect of ergocalciferol on the endothelium.

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“…In preclinical studies, the 5/6 nephrectomy was considered a good model for chronic kidney diseases. It was applied successfully in rats [ 14 , 15 ] and recently in dogs [ 16 ]. Few preclinical studies address the use of stem cells in large animal kidney injuries.…”

Section: Introductionmentioning

confidence: 99%

The therapeutic potential of Camel Wharton jelly mesenchymal stem cells (CWJ-MSCs) in canine chronic kidney disease model

et al. 2022

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Background Chronic kidney disease (CKD) is a worldwide health problem that its incidence increases nowadays with the increase in the risk of environmental pollution. CKD can progress to end-stage renal disease (ESRD) which usually ends fatally. This study aimed to examine the therapeutic potential of Camel Wharton jelly-mesenchymal stem cells (CWJ-MSCs) in chronic kidney disease model induced in dogs. Methods CWJ-MSCs were injected directed to the kidney with ultrasonographic guidance in dogs with 5/6 nephrectomy to evaluate its therapeutic potency in such cases. Analysis of variance was applied in normally distributed quantitative variables while a non-parametric Mann–Whitney test was used for non-normally distributed quantitative variables. Results The serum urea and creatinine in the treated group were significantly decreased transferring dogs in the treated group from stage 3 to stage 2 CKD according to the IRIS staging system. Histopathology of renal tissue revealed improving CKD lesions by increasing regeneration of degenerated tubules, maintaining the integrity of glomeruli. New vascularization with blood vessels remodeling were common findings. Periodic acid Schiff stain of renal tissue showed the integrity of renal tubules and thickness of the glomerular basement membrane. Fibrosis of cortex and medulla was lower in the treated group than in the CKD model as monitored by Mallory’s trichrome stain (MTC). NGAL and KIM-1 genes expression were decreased while VEGF and EGF genes expression were increased indicating renal tissue repair. Conclusions CWJ-MSCs have a therapeutic potential in the CKD model induced in dogs.

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Characterization of an experimental model of progressive renal disease in rats

Cited by 9 publications

References 27 publications

Development of Canine Chronic Kidney Disease Model: A Pilot Study

Development of Canine Chronic Kidney Disease Model: A Pilot Study

Ergocalciferol improves endothelial vasodilatory and vasoconstrictor function in an in vivo model of mild uraemia

The therapeutic potential of Camel Wharton jelly mesenchymal stem cells (CWJ-MSCs) in canine chronic kidney disease model

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