Effect of ozone and methylprednisolone treatment following crush type sciatic nerve injury

Abstract

PURPOSE:To assess and compare the histopathological effects of ozone therapy and/or methylprednisolone (MPS) treatment on regeneration after crush type sciatic nerve injury. METHODS:Forty Sprague-Dawley male rats were randomly allocated into four groups. Four groups received the following regimens intraperitoneally every day for 14 days after formation of crush type injury on sciatic nerve: Group I: ozone (20mcg/ml); Group II: methylprednisolone (2mg/kg); Group III: ozone (20 mcg/ml) and methylprednisolone (2mg/kg); Group IV: isotonic saline (0.9%). The histomorphological evaluation was made after biopsies were obtained from the sites of injury.RESULTS: Significant differences were noted between groups in terms of degeneration (p=0.019), nerve sheath cell atrophy (p=0.012), intraneural inflammatory cellular infiltration (p=0.002), perineural granulation tissue formation (p=0.019), perineural vascular proliferation (p=0.004), perineural inflammatory cellular infiltration (p<0.001) and inflammation in peripheral tissue (p=0.006).Degeneration was remarkably low in Group III, while no change in nerve sheath cell was noted in Group II. CONCLUSION:The combined use of methylprednisolone and ozone treatment can have beneficial effects for regeneration after crush type nerve injury.

Glucocorticoid attenuates acute lung injury through induction of type 2 macrophage

“…Therefore, it is widely used to treat a variety of acute and chronic inflammatory diseases including acute lung inflammation, asthma, and rheumatoid arthritis [1]. A number of studies have been reported for the anti-inflammatory properties of MP [2–4]. For instance, MP induces anti-inflammatory cytokines expression such as IL-10.…”

Cinnamaldehyde Enhanced Functional Recovery after Sciatic Nerve Crush Injury in Rats

“…Cells Tissues Organs 2020;209:43-53 DOI: 10.1159/000507016 regeneration and treatment of neuroinflammatory and neurodegenerative disorders [Chao et al, 2008]. Several exogenous factors, including antioxidants [Civi et al, 2016;Kocaoglu et al, 2017], neurotrophic agents [Li et al, 2017;Mesentier-Louro et al, 2019], hormonal supplements [Tuffaha et al, 2016;Moharrami Kasmaie et al, 2019], vitamins [Altun and Kurutas, 2016;Horasanli et al, 2017], neuroprotective drugs [Kian et al, 2019;Wang et al, 2019], anti-inflammatory drugs [Ozturk et al, 2016;Gao et al, 2017;Kaplan et al, 2018], physical factors [Chan et al, 2016;Daeschler et al, 2018], and medicinal plants [Constantin and Tache, 2012;Ho et al, 2013;Chen et al, 2015;Sayad-Fathi et al, 2019] have been examined as clinical treatments for peripheral nerve injury. The neuroprotective and inducing impacts of the exogenic agents could be efficiently employed in a clinical setting for peripheral nerve regeneration [Constantin and Tache, 2012].…”

Successful and Complete Recovery of the Ulnar Nerve After Eight Years of Chronic Injury Through Local Steroid Injections: A Case Report

“…Feng and Yuan demonstrated that dexamethasone can promote peripheral nerve repair in a rat model of sciatic nerve injury [16]. Furthermore, Ozturk et al concluded that the synergistic effect of methylprednisolone and ozone can have a beneficial effect regarding nerve regeneration after crush-type nerve injury [22].…”

“…Neuroactive steroidal substances have shown a promising neuroprotective effect in different experimental animal models [16,19,22]. In an experimental study conducted by Azcoitia et al, neuroactive steroids appeared to influence many physiological events involved in neural regeneration of rats' peripheral nerves, such as synthesis of myelin proteins, Schwann cell proliferation, and function of the axonal compartments through multiple signaling mechanisms [23].…”