Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR

Khosravi, Azar Dokht; Hoveizavi, Hajar; Mohammadian, Ali; Farahani, Abbas; Jenabi, Atefeh

doi:10.1590/s0102-865020160030000009

Cited by 29 publications

(21 citation statements)

References 18 publications

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“…Khosravi et al, also reported the high level of genotypic heterogeneity in P. aeruginosa strains isolated from this burn center have by ERIC-PCR analysis method (15).…”

Section: Discussionmentioning

confidence: 81%

Molecular Analysis of Pseudomonas aeruginosa Strains Isolated from Burn Patients by Repetitive Extragenic Palindromic-PCR (rep-PCR)

Sorkh

Shokoohizadeh

Rashidi

et al. 2017

Iran Red Crescent Med J

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Background: Infections caused by Pseudomonas aeruginosa raise an important issue in burn patients. Molecular epidemiologic studies have been used for investigating the genetic features of P. aeruginosa and rep-PCR technique has been introduced as a rapid low cost method. Objectives: This study focused on investigating the genetic similarity and antibiotic resistance pattern of P. aeruginosa isolated from the clinical samples of burn patients in a major burn center in Khuzestan Province, Iran. Methods: In a cross sectional study, a total of 75 strains of P. aeruginosa were isolated from burn patients at Taleghani hospital, which is the main burn center in Ahvaz, Iran, during May-September, 2015. Antimicrobial susceptibility of the isolates was detected using the disk diffusion method. Genetic relatedness of the isolates was analyzed by the rep-PCR technique. Results: Antimicrobial susceptibility testing showed more than 80% of P. aeruginosa isolates were resistant to ceftriaxone, cefotaxime, meropenem, piperacillin/tazobactam, ticarcillin, ciprofloxacin, and amikacin. Based on the rep-PCR analysis, 20 different common types and 20 unique patterns were illustrated among P. aeruginosa isolates. Conclusions: According to the findings of our study, there were diverse and high-level resistant P. aeruginosa strains in the major burn center in Khuzestan. Therefore, we faced troubles controlling the diverse P. aeruginosa clones in the burn patients.

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“…Khosravi et al, also reported the high level of genotypic heterogeneity in P. aeruginosa strains isolated from this burn center have by ERIC-PCR analysis method (15).…”

Section: Discussionmentioning

confidence: 81%

Molecular Analysis of Pseudomonas aeruginosa Strains Isolated from Burn Patients by Repetitive Extragenic Palindromic-PCR (rep-PCR)

Sorkh

Shokoohizadeh

Rashidi

et al. 2017

Iran Red Crescent Med J

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“…DNA was extracted from each fecal sample with a bacterial genomic DNA extraction kit (QIAgen, Germany). PCR amplification was performed on extracted DNA as described by Khosravi et al [22] using the primers ERIC1 () and ERIC2 () in a PCR amplification instrument (Bio-Rad, USA). The reactions were performed in a final volume of 25 μL containing 2 μL of template DNA, 0.5 mL of each ERIC primer, 0.2 μL of 5 U/μL Taq DNA polymerase, 2 μL of 2.5 mmol dNTP, 2.5 μL of 10 × buffer and 17.3 μL nuclease-free water.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats

Xue¹,

Liu²,

Lyu³

et al. 2017

PLoS ONE

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BackgroundThis study investigated the protective effect of aplysin on the liver and its influence on inflammation and the gut microbiota in rats with ethanol-induced liver injury.MethodsMale Sprague-Dawley rats were randomly assigned to an alcohol-containing liquid diet, control liquid diet or treatment with aplysin for 8 weeks. Hepatic and intestinal histopathological analysis was performed, and cytokine levels and the intestinal mucosal barrier were assessed. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) and 16S rDNA high-throughput sequencing were performed to provide an overview of the gut microbiota composition.ResultsChronic alcohol exposure caused liver damage in rats. Serum aspartate aminotransferase (AST), aminotransferase (ALT), alkaline phosphatase (ALP) and triglyceride (TG) activities in liver tissue were higher than in the control group. Alcohol administration elevated the levels of serum transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) and reduced interleukin-10 (IL-10) levels compared with those of control rats. In addition, the levels of plasma endotoxin, diamine oxidase (DAO), and fatty acid-binding protein 2 (FABP2) in the alcohol group were higher than in the control group. The results of ERIC-PCR indicated that aplysin treatment shifted the overall structure of the ethanol-disrupted gut microbiota toward that of the control group. One hundred twenty to 190 genera of bacteria were detected by high throughput sequencing. Alcohol-induced changes in the gut microbial composition were detected at the genus level. These alcohol-induced effects could be reversed with aplysin treatment.ConclusionsThese results suggest that aplysin exerts a protective effect on ethanol-induced hepatic injury in rats by normalizing fecal microbiota composition and repairing intestinal barrier function.

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“…To test the genetic diversity of our collected strains, we performed Enterobacteriaceae repetitive intergenic consensus polymerase chain reaction (ERIC-PCR)24. Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…PCR amplification was performed with the following primers24. ERIC1 (5′-ATGTAAGCTCCTGGGGATTCAC-3′) and ERIC2 (5′-AAGTAAGTACTGGGGTGAGCG-3′).…”

Section: Methodsmentioning

confidence: 99%

“…The DNA banding patterns were entered into a database in ImageLab 2.0 software (BIO-RAD, USA) to automatically obtain and analyze the images. The ERIC-PCR patterns were interpreted and compared as previously described24. Similarity analysis was calculated with the Dice coefficient and the unweighted pair group average (UPGMA) for cluster analyses.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of the first double-stranded RNA bacteriophage infecting Pseudomonas aeruginosa

Yang

Shen

et al. 2016

Sci Rep

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Bacteriophages (phages) are widely distributed in the biosphere and play a key role in modulating microbial ecology in the soil, ocean, and humans. Although the role of DNA bacteriophages is well described, the biology of RNA bacteriophages is poorly understood. More than 1900 phage genomes are currently deposited in NCBI, but only 6 dsRNA bacteriophages and 12 ssRNA bacteriophages genome sequences are reported. The 6 dsRNA bacteriophages were isolated from legume samples or lakes with Pseudomonas syringae as the host. Here, we report the first Pseudomonas aeruginosa phage phiYY with a three-segmented dsRNA genome. phiYY was isolated from hospital sewage in China with the clinical P. aeruginosa strain, PAO38, as a host. Moreover, the dsRNA phage phiYY has a broad host range, which infects 99 out of 233 clinical P. aeruginosa strains isolated from four provinces in China. This work presented a detailed characterization of the dsRNA bacteriophage infecting P. aeruginosa.

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Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR

Cited by 29 publications

References 18 publications

Molecular Analysis of Pseudomonas aeruginosa Strains Isolated from Burn Patients by Repetitive Extragenic Palindromic-PCR (rep-PCR)

Molecular Analysis of Pseudomonas aeruginosa Strains Isolated from Burn Patients by Repetitive Extragenic Palindromic-PCR (rep-PCR)

Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats

Characterization of the first double-stranded RNA bacteriophage infecting Pseudomonas aeruginosa

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