Evaluation of the effect of allopurinol as a protective factor in post ischemia and reperfusion inflammation in Wistar rats

Gomes, Ricardo Zanetti; Romanek, Gabriela Moreira Mahle; Przybycien, Michella; Amaral, Danielli Cristina; Akahane, Hugo Genki Kagawa

doi:10.1590/s0102-865020160020000007

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…TNF-a (tumor necrosis factor-a) is an inflammatory marker secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial lipopolysaccharides (Gomes et al, 2016). By performing pairwise comparison among the six tested groups, The TNF-a level showed significant difference among the six tested groups (p50.05).…”

Section: Resultsmentioning

confidence: 99%

Comparative pharmaceutical study on colon targeted micro-particles of celecoxib:in-vitro–in-vivoevaluation

et al. 2016

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In order to target celecoxib which is a COX2 inhibitor, with potentials in the prevention and treatment of colitis and colon cancer, it was formulated as microparticles using the solvent/ evaporation method and various pH-dependent Eudragit polymers. The in-vitro evaluation of the prepared microparticles showed spherical and smooth morphology. The encapsulation efficiency and yield were high, indicating that the method used is simple and efficient at this scale. The in-vitro release study showed no release in the acidic medium for 2 h followed by the release of the drug in pH 6.8 in case of Eudragit L100-55 and L100 and pH 7.4 in case of Eudragit S100. The pharmacokinetic parameters were calculated and method validation was performed to insure that it is suitable and reliable. Pharmacokinetic parameters were investigated by determining the C max , T max , AUC 0-t , K el , and t 1/2 of the drug as a suspension and as microparticles. There was a significant difference (p50.05) in T max between the drug as a suspension and as microparticles. The effect of celecoxib on the degree of inflammation was examined on acetic acid induced colitis rat model and the drug was given as a suspension and as microparticles. The evaluation was done using macroscopical, microscopical and biochemical examination. There was a significant difference between the acetic acid control group and the treatment groups regarding all examination criteria in the order microparticles formulated using Eudragit S100 followed by Eudragit L100-55 while microparticles using Eudragit L100 and drug suspension showed almost the same results.

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Section: Resultsmentioning

confidence: 99%

Comparative pharmaceutical study on colon targeted micro-particles of celecoxib:in-vitro–in-vivoevaluation

et al. 2016

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“…In contrast, there are also studies in which its oral administration prior to ischemia instauration does demonstrate an antioxidant effect, as evidenced by a reduction in HID and a decrease in the production of oxidative stress markers such as malondialdehyde (MDA) [33]. Other studies performed in a renal IRI mode have shown the beneficial effect of XO inhibition [34][35][36][37][38]. After prophylactic administration of allopurinol, a decrease of up to 75% in the damage was noticed, both biochemical and anatomopathological.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

et al. 2021

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Intestinal ischemia-reperfusion injury (i-IRI) is a rare disorder with a high mortality rate, resulting from the loss of blood flow to an intestinal segment. Most of the damage is triggered by the restoration of flow and the arrival of cytokines and reactive oxygen species (ROS), among others. Inactivation of these molecules before tissue reperfusion could reduce intestinal damage. The aim of this work was to analyze the preventive effect of allopurinol and nitroindazole on intestinal mucosal damage after i-IRI. Wag/RijHsd rats were subjected to i-IRI by clamping the superior mesenteric artery (for 1 or 2 h) followed by a 30 min period of reperfusion. Histopathological intestinal damage (HID) was assessed by microscopic examination of histological sections obtained from injured intestine. HID was increased by almost 20% by doubling the ischemia time (from 1 to 2 h). Nitroindazole reduced HID in both the 1 and 2 h period of ischemia by approximately 30% and 60%, respectively (p < 0.001). Our preliminary results demonstrate that nitroindazole has a preventive/protective effect against tissue damage in the early stages of i-IRI. However, to better understand the molecular mechanisms underlying this phenomenon, further studies are needed.

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“…Gomes et al 18 demonstrates I/R-induced inflammation reduction, when comparing the efficiency of allopurinol in reducing serial concentration of tumor necrosis factors, of the I/R group, with groups submitted to I/R and treated with allopurinol. Allopurinol also displayed significant advantages in reducing injury by reperfusion in the kidneys of rats, which was demonstrated by Keel et al 19 , by the utilization of 8-isoprostane as oxidative stress marker in the evaluation of injury by I/R; Zhou et al 20 evidenced improvement in kidney function and histology.…”

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confidence: 99%

The influence of allopurinol and post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion in Wistar rats

Akahane

Gomes²,

Paludo³

et al. 2017

Acta Cir. Bras.

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1 AbstractPurpose: To analyze the effects of allopurinol and of post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion. Methods: Thirty rats were used. They were divided in 5 groups: (1) group A: abdominal aortic dissection only, (2) group B: ischemia and reperfusion, (3) group C: administered allopurinol (100mg/Kg) a few hours before procedure, (4) group D: post-conditioned and (5) group E: administered allopurinol and post-conditioned. With the exception of group A, all groups were submitted to infrarenal aortic ischemia for 2 hours, and reperfusion for 72 hours. After euthanasia, lungs were removed for histological analysis. They were graded under two scores: pulmonary injury (neutrophil infiltration, interstitial edema, vascular congestion, and destruction of lung architecture) and lymphocytic score (neutrophil infiltration, lymphoid aggregate and secondary follicle). Results: On the pulmonary injury score, the degree of injury was smaller than in groups D and E, when compared to group B, p<0.05. Group C did not obtain the same result (p>0,05). On the lymphocytic score, there was no statistic difference among groups, p>0.05. Conclusion: Both post-conditioning and the combination of allopurinol and post-conditioning were effective in remote lung protection induced by lower-limbs I/R. When used in isolation, allopurinol showed no protective effect.

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Evaluation of the effect of allopurinol as a protective factor in post ischemia and reperfusion inflammation in Wistar rats

Cited by 8 publications

References 22 publications

Comparative pharmaceutical study on colon targeted micro-particles of celecoxib:in-vitro–in-vivoevaluation

Comparative pharmaceutical study on colon targeted micro-particles of celecoxib:in-vitro–in-vivoevaluation

Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

The influence of allopurinol and post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion in Wistar rats

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