Ultrasound biomicroscopy and claudication test for in vivo follow-up of muscle repair enhancement based on platelet-rich plasma therapy in a rat model of gastrocnemius laceration

Pinheiro, Cristiane Lima; Peixinho, Carolina Carneiro; Esposito, Cristiano Costa; Manso, José Eduardo Ferreira; Machado, João Carlos

doi:10.1590/s0102-865020160020000004

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…Of the 2 studies that examined both the in vivo and in vitro uses of PRP, one used human muscle-derived progenitor cells (hMDPCs) from unspecified skeletal muscle from living and post-mortem biopsies for the in vitro component[26], while the other used an established murine myogenic cell line (C2C12)[27] (Table 6). Only one study reported platelet count and WBC concentration[24], while neither reported RBC count. For the in vivo component, the gastrocnemius muscle of a mouse[26] and the rotator cuff muscles (supraspinatus and infraspinatus) of a rat[27] were used.…”

Section: Resultsmentioning

confidence: 99%

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Kunze¹,

Hannon²,

Fialkoff³

et al. 2019

WJO

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BACKGROUND Platelet-rich plasma (PRP) is an increasingly used biologic adjunct for muscle injuries, as it is thought to expedite healing. Despite its widespread use, little is known regarding the mechanisms by which PRP produces its efficacious effects in some patients. AIM To clarify the effects of PRP on muscular pathologies at the cellular and tissue levels by evaluating the basic science literature. METHODS A systematic review of PubMed/MEDLINE and EMBASE databases was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and checklist. Level III in vivo and in vitro studies examining PRP effects on muscles, myocytes and/or myoblasts were eligible for inclusion. Extracted data included PRP preparation methods and study results. RESULTS Twenty-three studies were included (15 in vivo , 6 in vitro , 2 in vitro / in vivo ). Only one reported a complete PRP cytology (platelets, and red and white blood cell counts). Five in vitro studies reported increased cellular proliferation, four reported increased gene expression, and three reported increased cellular differentiation. Five in vivo studies reported increased gene expression, three reported superior muscle regeneration, and seven reported improved histological quality of muscular tissue. CONCLUSION The basic science literature on the use of PRP in muscle pathology demonstrates that PRP treatment confers several potentially beneficial effects on healing in comparison to controls. Future research is needed to determine optimal cytology, dosing, timing, and delivery methods of PRP for muscle pathologies.

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Section: Resultsmentioning

confidence: 99%

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Kunze¹,

Hannon²,

Fialkoff³

et al. 2019

WJO

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“…Despite the limitations in human trials, with respect to PRP, there have been numerous progressions in the field of skeletal muscle regeneration in rodent models . Accumulating data from animal studies on the role of PRP on skeletal muscle recovery after varying types of injury has emerged (Table ).…”

Section: Platelet‐mediated Skeletal Muscle Regenerationmentioning

confidence: 99%

“…These factors in turn upregulate proliferation, differentiation and migration of necessary cells in the area of regenerating tissue . Over the past decade, there have been amounting articles contributing to the knowledge surrounding the mechanisms of growth factors in the regeneration of wounded or dysfunctional tissue . Due to increasing understanding in cell signalling and growth factor biology, research and clinical attention has been drawn to the use of autologous PRP as a novel means of delivering growth factors to injured tissue such as liver, bone and skeletal muscle (See Table : muscle tissue regeneration , Table : other tissue regeneration and Table : in vitro cell studies).…”

Section: Introductionmentioning

confidence: 99%

Platelet biology in regenerative medicine of skeletal muscle

2018

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Platelet-based applications such as platelet-rich plasma (PRP) and platelet releasate have gained unprecedented attention in regenerative medicine across a variety of tissues as of late. The rationale behind utilizing PRP originates in the delivery of key cytokines and growth factors from α-granules to the targeted area, which in turn act as cell cycle regulators and promote the healing process across a variety of tissues. The aim of the present review is to assimilate current experimental evidence on the role of platelets as biomaterials in tissue regeneration, particularly in skeletal muscle, by integrating findings from human, animal and cell studies. This review is composed of 3 parts: firstly, we review key aspects of platelet biology that precede the preparation and use of platelet-related applications for tissue regeneration. Secondly, we critically discuss relevant evidence on platelet-mediated regeneration in skeletal muscle focusing on findings from (i) clinical trials, (ii) experimental animal studies and (iii) cell culture studies; and thirdly, we discuss the application of platelets in the regeneration of several other tissues including tendon, bone, liver, vessels and nerve. Finally, we review key technical variations in platelet preparation that may account for the large discrepancy in outcomes from different studies. This review provides an up-to-date reference tool for biomedical and clinical scientists involved in platelet-mediated tissue regenerative applications.

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“…In addition, the reduction of type-I collagen deposition and scar formation (fibrosis) [106,110,112,113,115,116,117,118] the enhancement of angiogenesis [106,110,112,116,117] and a faster functional recovery [108,109,113,118,119] have been also observed after PRP application on damaged muscles. Takase and co-workers [120] recently demonstrated also the ability of PRP to prevent fatty degenerative changes of rotator cuff muscles in a rat rotator cuff tear model, when administered into subacromial space.…”

Section: Plasma Preparations: Platelet-rich Plasma (Prp) and Platementioning

confidence: 99%

Influence of Platelet-Rich and Platelet-Poor Plasma on Endogenous Mechanisms of Skeletal Muscle Repair/Regeneration

Chellini

Tani

Zecchi‐Orlandini

et al. 2019

IJMS

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The morpho-functional recovery of injured skeletal muscle still represents an unmet need. None of the therapeutic options so far adopted have proved to be resolutive. A current scientific challenge remains the identification of effective strategies improving the endogenous skeletal muscle regenerative program. Indeed, skeletal muscle tissue possesses an intrinsic remarkable regenerative capacity in response to injury, mainly thanks to the activity of a population of resident muscle progenitors called satellite cells, largely influenced by the dynamic interplay established with different molecular and cellular components of the surrounding niche/microenvironment. Other myogenic non-satellite cells, residing within muscle or recruited via circulation may contribute to post-natal muscle regeneration. Unfortunately, in the case of extended damage the tissue repair may become aberrant, giving rise to a maladaptive fibrotic scar or adipose tissue infiltration, mainly due to dysregulated activity of different muscle interstitial cells. In this context, plasma preparations, including Platelet-Rich Plasma (PRP) and more recently Platelet-Poor Plasma (PPP), have shown advantages and promising therapeutic perspectives. This review focuses on the contribution of these blood-derived products on repair/regeneration of damaged skeletal muscle, paying particular attention to the potential cellular targets and molecular mechanisms through which these products may exert their beneficial effects.

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Ultrasound biomicroscopy and claudication test for in vivo follow-up of muscle repair enhancement based on platelet-rich plasma therapy in a rat model of gastrocnemius laceration

Cited by 5 publications

References 27 publications

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Platelet biology in regenerative medicine of skeletal muscle

Influence of Platelet-Rich and Platelet-Poor Plasma on Endogenous Mechanisms of Skeletal Muscle Repair/Regeneration

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