The influence of low-level laser irradiation on spinal cord injuries following ischemia- reperfusion in rats

Sotoudeh, Ahmad; Jahanshahi, Amirali; Zareiy, S; Darvishi, Mohammad; Roodbari, Nasim Hayati; Bazzazan, Ali

doi:10.1590/s0102-865020150090000005

Cited by 18 publications

(4 citation statements)

References 32 publications

(33 reference statements)

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“…670 nm treatment has shown to significantly reduce neuronal cell death following SCI from 1-dpi. A similar study using 810 nm treatment showed 50% less motor neuron death following ischemia-induced SCI at 3-dpi 60 . The mechanism by which 670 nm treatment reduces neuronal cell death might be related to reduced iNOS ( Fig 9 ).…”

Section: Discussionmentioning

confidence: 71%

Red-light (670 nm) therapy reduces mechanical sensitivity and neuronal cell death, and alters glial responses following spinal cord injury in rats

Moalem‐Taylor

Potas

2020

Preprint

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Individuals with spinal cord injury (SCI) often develop debilitating neuropathic pain, which may be driven by neuronal damage and neuroinflammation. We have previously demonstrated that treatment using 670 nm (red) light irradiation alters microglia/macrophage responses and alleviates mechanical hypersensitivity at 7-days post-injury. Here, we investigated the effect of red-light on the development of mechanical hypersensitivity, neuronal markers, and glial response in the subacute stage (days 1-7) following SCI. Wistar rats were subjected to a mild T10 hemi-contusion SCI or sham surgery followed by daily red-light treatment (30 min/day; 670 nm LED; 35mW/cm2) or sham treatment. Mechanical sensitivity of the rat dorsum was assessed from 1-day post-injury and repeated every second day. Spinal cords were collected at 1, 3, 5 and 7-days post-injury for analysis of myelination, neurofilament protein NF200 expression, neuronal cell death, reactive astrocytes (GFAP+ cells), interleukin1β (IL1β) expression, and inducible nitric oxide synthase (iNOS) production in IBA1+ microglia/macrophages. Red-light treatment significantly reduced the cumulative mechanical sensitivity and the hypersensitivity incidence following SCI. This effect was accompanied by significantly reduced neuronal cell death, reduced astrocyte activation and reduced iNOS expression in IBA1+ cells at the level of the injury. However, myelin and NF200 immunoreactivity and IL1β expression in GFAP+ and IBA1+ cells were not altered by red-light treatment. Thus, red-light therapy may represent a useful non-pharmacological approach for treating pain during the subacute period after SCI by decreasing neuronal loss and modulating the inflammatory glial response.

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Section: Discussionmentioning

confidence: 71%

Red-light (670 nm) therapy reduces mechanical sensitivity and neuronal cell death, and alters glial responses following spinal cord injury in rats

Moalem‐Taylor

Potas

2020

Preprint

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“…All rats were deeply anesthetized with sodium pentobarbital (25 mg/kg) and perfused with saline. Spinal cord sections from the tenth thoracic segment were dissected totally and fixed in 10% formalin and embedded in paraffin using routine procedures 37 . Serial transverse sections (5 μm) were cut from paraffin blocks and stained with HE for histopathologic examination under a light microscope (Leica DM4000 M, Wetzlar, Germany).…”

Section: Methodsmentioning

confidence: 99%

Intravenous transplantation of olfactory bulb ensheathing cells for a spinal cord hemisection injury rat model

et al. 2019

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Cellular transplantation strategies utilizing intraspinal or intrathecal olfactory ensheathing cells (OECs) have been reported as beneficial for spinal cord injury (SCI). However, there are many disadvantages of these methods, including additional trauma to the spinal cord parenchyma and technical challenges. Therefore, we investigated the feasibility and potential benefits of intravenous transplantation of OECs in a rat hemisection SCI model. OECs derived from olfactory bulb tissue were labeled with quantum dots (QDs), and their biodistribution after intravenous transplantation was tracked using a fluorescence imaging system. Accumulation of the transplanted OECs was observed in the injured spinal cord within 10 min, peaked at seven days after cell transplantation, and decreased gradually thereafter. This time window corresponded to the blood–spinal cord barrier (BSCB) opening time, which was quantitated with the Evans blue leakage assay. Using immunohistochemistry, we examined neuronal growth (GAP-43), remyelination (MBP), and microglia (Iba-1) reactions at the lesion site. Motor function recovery was also measured using a classic open field test (Basso, Beattie and Bresnahan score). Compared with the group injected only with QDs, the rats that received OEC transplantation exhibited a prominent reduction in inflammatory responses, increased neurogenesis and remyelination, and significant improvement in motor function. We suggest that intravenous injection could also be an effective method for delivering OECs and improving functional outcomes after SCI. Moreover, the time course of BSCB disruption provides a clinically relevant therapeutic window for cell-based intervention.

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“…Experimental data have suggested nervous tissue regeneration by laser-induced photobiomodulation in neurons. In fact, a low-power infrared (810 nm) laser was able to promote axonal regeneration and functional recovery in rats affected by spinal cord injury caused by different types of trauma in experimental models [92][93][94][95]. Also, mechanisms of Ca 2+dependent BDNF mRNA transcription could be triggered by exposure to a low-power red (632,8 nm) laser in primary neuron cultures from rat spinal cord [96].…”

Section: Could Low-power Lasers Be Effective On Amblyopic Eyes?mentioning

confidence: 99%

Low-power lasers on amblyopia

Paiva

Fonseca

2019

Laser Phys. Lett.

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Amblyopia is one of the most important causes of visual impairment that presents as incorrect image forming in the fovea of the affected eye, and it occurs with high incidence during childhood. It is a neural disorder that occurs as a disruption of binocular vision during early childhood, with no abnormality in the optic pathways. The type and severity of amblyopia depends on the critical period, when the highest rates of neural plasticity are observed; this being considered ideal for treatment. It is possible that modulation of neural plasticity in the visual cortex could be probed as an alternative approach for the treatment of amblyopia. Low-power lasers are devices emitting laser beams with power from 1 up to 100 mW and wavelengths ranging between red and near-infrared radiation. These lasers are widely used in regenerative medicine for modulation of wound healing, anti-inflammatory action and pain, but they are also considered for treatment of retinal diseases, stroke, neurotrauma, neurodegeneration, memory and mood disorders, as well as cognitive impairment. However, few data are available about their clinical applications for treatment of amblyopia. Experimental models for amblyopia could be used to determine the adequate laser irradiation conditions, and important results could be obtained, thus confirming and providing a scientific basis for a therapeutic protocol for amblyopia based on low-power lasers.

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The influence of low-level laser irradiation on spinal cord injuries following ischemia- reperfusion in rats

Cited by 18 publications

References 32 publications

Red-light (670 nm) therapy reduces mechanical sensitivity and neuronal cell death, and alters glial responses following spinal cord injury in rats

Red-light (670 nm) therapy reduces mechanical sensitivity and neuronal cell death, and alters glial responses following spinal cord injury in rats

Intravenous transplantation of olfactory bulb ensheathing cells for a spinal cord hemisection injury rat model

Low-power lasers on amblyopia

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