Parecoxib reduces renal injury in an ischemia/reperfusion model in rats

Neto, José Pedro Calistro; Torres, Rômulo da Costa; Gonçalves, Giovanna Maria; Silva, Leopoldo Muniz da; Domingues, Maria Aparecida Custódio; Módolo, Norma Sueli Pinheiro; Barros, Guilherme Antônio Moreira de

doi:10.1590/s0102-865020150040000006

Cited by 9 publications

(4 citation statements)

References 18 publications

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“…A possible mechanism underlying the role of Pare in I/R injury may lie in its inhibitory effects on COX‐2 and prostaglandin synthesis 47 . A number of studies have demonstrated the protective effects of Pare on various tissues in animal models of I/R injury 11–14 . Such a protective role of Pare is mainly mediated through anti‐inflammation, antioxidation, as well as the regulation of apoptosis and autophagy, 11–14 highlighting the superiority and potential values of Pare in clinical application.…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies have demonstrated the protective effects of Pare on various tissues in animal models of I/R injury 11–14 . Such a protective role of Pare is mainly mediated through anti‐inflammation, antioxidation, as well as the regulation of apoptosis and autophagy, 11–14 highlighting the superiority and potential values of Pare in clinical application. However, the potential role of Pare in pulmonary I/R injury and the underlying mechanisms have rarely been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have demonstrated that Pare plays a protective role in various organs, including the brain, 11 heart, 12 liver, 13 and kidneys 14 in I/R animal models. Such a protective role of Pare is mainly mediated via anti‐inflammation, anti‐oxidation, as well as the regulation of apoptosis, 11–14 highlighting its superiority and potential value in clinical application. It has been reported that Pare can prevent I/R injury in a dose‐dependent manner, possibly by suppressing the inflammatory reaction and apoptosis of intestinal mucosal epithelial cells 15 .…”

Section: Introductionmentioning

confidence: 99%

“…Parecoxib sodium (Pare), a nonsteroidal anti‐inflammatory drug commonly used for postoperative pain, is a selective inhibitor of COX‐2 10 . Numerous studies have demonstrated that Pare plays a protective role in various organs, including the brain, 11 heart, 12 liver, 13 and kidneys 14 in I/R animal models. Such a protective role of Pare is mainly mediated via anti‐inflammation, anti‐oxidation, as well as the regulation of apoptosis, 11–14 highlighting its superiority and potential value in clinical application.…”

Section: Introductionmentioning

confidence: 99%

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Parecoxib sodium alleviates ischemia reperfusion‐induced pulmonary injury via inhibiting ERK/NF‐κB and further activating the HIF‐1α pathway

Guo

Yang

2022

Immunity Inflam & Disease

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Introduction The lungs are extremely vulnerable to ischemia/reperfusion (I/R), which is characterized by intense inflammation, oxidative stress, alveolar damage, and vascular permeability. Parecoxib sodium (Pare) has been shown to exert protective effects against multiple I/R‐induced tissue injuries. However, its role in I/R‐induced lung injury remains unknown. This study aimed to reveal the roles and mechanisms of Pare in pulmonary I/R injury. Methods Sixty‐six rats were randomly divided into three groups: The sham‐operated group, the pulmonary I/R group, and the Pare‐pretreated I/R group. Pare at 10 mg/kg or saline (vehicle control) were intraperitoneally administered to rats once per day for 5 consecutive days before ischemia. Serum and tissue samples were harvested following 2 h of reperfusion. The oxygenation index (OI) and alveolar‐arterial oxygen partial pressure difference (PA‐aO2) were analyzed. The levels or activities of malondialdehyde, superoxidase dismutase, catalase, glutathione peroxidase, intracellular reactive oxygen species, tumor necrosis factor‐α, interleukin (IL)‐6, and IL‐8 were examined. The mitochondrial membrane potential was measured. The protein expression levels of the extracellular signal‐regulated kinase (ERK), nuclear factor‐κB (NF‐κB) and their phosphorylated forms, and hypoxia‐inducible factor‐1α (HIF‐1α) were detected. Histological changes were observed using hematoxylin and eosin staining. Moreover, the survival rate following pulmonary I/R injury was recorded daily. Results Pare significantly increased the OI, decreased the PA‐aO2, increased the levels of antioxidants, while decreasing the levels of oxidants, and alleviated mitochondrial dysfunction and the histopathological damage induced by I/R. Furthermore, Pare inhibited the expression of proinflammatory cytokines, suppressed the activation of ERK and NF‐κB, further increased HIF‐1α expression, and significantly improved the rat survival rate. Conclusions Pare pretreatment attenuated lung I/R injury by inhibiting oxidative stress and the inflammatory response possibly via inhibiting the activation of the ERK/NF‐κB pathway and further activating the HIF‐1α pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Parecoxib sodium alleviates ischemia reperfusion‐induced pulmonary injury via inhibiting ERK/NF‐κB and further activating the HIF‐1α pathway

Guo

Yang

2022

Immunity Inflam & Disease

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Preparation and Monitoring of Small Animals in Renal MRI

Kaucsár

Hosszú

Seeliger

et al. 2021

Methods in Molecular Biology

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Renal diseases remain devastating illnesses with unacceptably high rates of mortality and morbidity worldwide. Animal models are essential tools to better understand the pathomechanism of kidney-related illnesses and to develop new, successful therapeutic strategies. Magnetic resonance imaging (MRI) has been actively explored in the last decades for assessing renal function, perfusion, tissue oxygenation as well as the degree of fibrosis and inflammation. This chapter aims to provide an overview of the preparation and monitoring of small animals before, during, and after surgical interventions or MR imaging. Standardization of experimental settings such as body temperature or hydration of animals and minimizing pain and distress are essential for diminishing nonexperimental variables as well as for conducting ethical research.This publication is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers.

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