Low-level laser therapy modulates musculoskeletal loss in a skin burn model in rats

Abstract

PURPOSE:To investigate the effectiveness of low-level laser therapy (LLLT) on gastrocnemius muscle morphology and Myod imunoexpression in a model of dorsal burn in rats. METHODS:Sixteen male Wistar rats were distributed into two groups: control group (CG): rats submitted to scald burn injury without treatment and laser treated group (LG): rats submitted to scald burn injury and treated with laser therapy. Fourteen days post-surgery, gastrocnemius muscle was evaluated being the specimens stained with HE and morphometric data was evaluated. MyoD expression was assessed by immunohistochemistry. RESULTS:The results showed that laser treated animals presented more organized tissue morphology compared to the non-treated animals, with a higher number of nucleus in the fibers. Also, the cross sectional area of the fibers and the MyoD immunoexpression in the laser treated groups was higher.CONCLUSION: Low-level laser therapy had positive effects on gastrocnemius muscle, improving tissue muscle morphology, increasing cross sectional area and MyoD immunoexpression.Key words: Burns. Laser Therapy, Low-Level, MyoD Protein. Rats. Low-level laser therapy modulates musculoskeletal loss in a skin burn model in ratsActa Cirúrgica Brasileira -Vol. 30 (2) 2015 -95

Low-level laser therapy (LLLT) accelerates the sternomastoid muscle regeneration process after myonecrosis due to bupivacaine

“…As infrared wavelength was used in the application of LLLT, the absorption occurs through the cytochrome c oxidase (COX) [ 67,85], which is the principal chromophore in cells, increasing mitochondrial products such as ATP, NADH, proteins and RNA and a reciprocal increase in oxygen consumption occurs [86]. All of these physiologic modifications induced by LLLT could have stimulated mitochondrial physiology due to lesions caused by the anesthetic, reducing the levels of muscle catabolism and loss of proteins and promoting regeneration and organization of muscle tissue [87], which may explain the increased number of nuclei observed in the LBupi subgroup in the present study.…”

Laser parameters for efficient biomedical stimulation: A study to increase cognitive response rate

“…Development of laser-related equipment is actively underway for diagnosis, treatment, and clinical research. In particular, laser-related research has been actively carried out to relieve or treat diseases using skin and nerve stimulation principles [ 1 , 2 , 3 ]. Recently, research on tactile stimulation using lasers has been attempted [ 4 ].…”

Anti-inflammatory effects of low-level laser in burn wound models in rats

“…Some benefits of low intensity laser therapy include microcirculation enhancement, activation of angiogenesis, vasodilatation, enhanced tissue metabolism and analgesic effects histologically [12][13][14]. Deep penetration of low level laser photons into the body through the skin, nerves, tendons, muscles, ligaments, and even bones can occur to address the painful and injured areas [15].…”