Anti-hemorrhagic effect of hydro-alcoholic extract of the leaves of Mikania glomerata in lesions induced by Bothrops jararaca venom in rats

Abstract

Conception, design, intellectual and scientific content of the study; critical analysis; final approval. ABSTRACT PURPOSE:To investigate the effects of hydroalcoholic leaf extract of Mikania glomerata Spreng (Asteraceae) on the activity of Bothrops jararaca snake venom in Wistar rats. METHODS:Fifty four rats Wistar were divided into six groups of nine animals in each: control treated with saline; control treated with B. jararaca venom; control treated with M. glomerata extract; B. jararaca venom incubated with M. glomerata extract at proportions of 1:1, 1:2, and 1:4. RESULTS: Histopathological and morphometric analysis showed that intradermal administration of snake venom incubated with the hydroalcoholic extract at proportions of 1:1, 1:2 and 1:4 promoted a significant reduction in the number of inflammatory cells and a marked decrease in edema after the third hour. There was also a significant reduction in the intensity of the hemorrhagic halo in animals receiving the snake venom incubated with the extract, with the observation of a progressive and parallel inhibition with increasing proportion of M. glomerata. CONCLUSION:The Mikania glomerata hydroalcoholic extract exerted effective anti-inflammatory and antihemorrhagic activity against the effects induced by Bothrops jararaca snake venom.

Effects of Mikania glomerata Leaf Extract on Experimental Bothropoides jararaca Envenomation in Wistar Rats

“…These findings agree with experimentally identified anti-inflammatory and antihemorrhagic properties of the Mikania genus against myotoxic, neurotoxic, and inflammatory effects of snake venoms [2]. The above observations are further supported by the findings of other studies in rats that revealed 80% reduction in hemorrhage area following intradermal injection of Bothrops venom and Mikania extract administration [14], and reduction of inflammatory cells, edema, and hemorrhagic halo 3 h after administration of B. jararaca venom and Mikania leaf extract [10].…”

Anti-inflammatory actions of herbal medicines in a model of chronic obstructive pulmonary disease induced by cigarette smoke

“…Our results are in line with researches that pointed the protective role of M. glomerata in the prevention of the oxidative lung damage in a coal dust exposure model [17] and reduction of inflammatory cells in lesions induced by Bothrops jararaca venom in rats [26].…”

Comparative morphoanatomical analysis of Mikania species

“…Mikania spp. has provided a wide range of promising phamacological activities and it can be described as having expectorant, anti-inflammatory, bronchodilator, anti-hemorrhagic, antiasthmatic, analgesic, anti-mutagenic, trypanocidal, antimicrobial, antifungal, antiulcerogenic, muscle relaxant, and antirheumatic properties (Muelas-Serrano et al, 2000;Paul et al, 2000;Gasparetto et al, 2010;Pérez-Amador et al, 2010;Mourão et al, 2014;Ríos et al, 2014).…”