Effects of hyperbaric oxygen (HBO), as pre-conditioning in liver of rats submitted to periodic liver ischemia/reperfusion

Caldeira, Diego Elias da Silva; Souza, Maria Eliza Jordani de; Gomes, Maria Cecília Jordani; Picinato, M A N C; Fina, Clarice Fleury; Féres, Omar; Silva, Orlando Castro e

doi:10.1590/s0102-86502013001300013

Cited by 11 publications

(19 citation statements)

References 17 publications

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“…Although expected, no difference was observed with HBO. This fact was not observed in other studies, in which the SHAM and IR groups already presented some difference [10][11][12]16 . In those studies there was an increase in the rate of oxygen consumption by the mitochondria compared to the SHAM group in order to maintain the electrochemical proton gradient on the mitochondrial membrane so that the membrane would be able to phosphorylate ADP 16,17 .…”

Section: Discussioncontrasting

confidence: 80%

“…This complex that affects the liver in various surgical procedures such as liver transplantation 15 is being investigated in order to find some way of attenuating its adverse effects. In an attempt to contribute to a greater number of possibilities for the attenuation of the undesirable effects of the procedure, we performed the surgical procedure of hepatic I/R followed by submission of the experimental animals to high oxygen pressures in a hyperbaric chamber 10,12,16 In the present study, the production of energy by the mitochondria was analyzed starting from State 3. Regarding this analysis, we may state that the animals submitted to HBO after hepatic I/R showed an improvement when compared to the rats submitted only to I/R.…”

Section: Discussionmentioning

confidence: 99%

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Effects of hyperbaric oxygen therapy on the liver after injury caused by the hepatic ischemia-reperfusion process

et al. 2014

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PURPOSE:To evaluate the effects of hyperbaric oxygen on rats submitted to hepatic ischemia and reperfusion. METHODS:Twenty-three Wistar rats were divided at random into 3 groups: SHAM, rats submitted to surgical and anesthetic stress without induction of hepatic ischemia/reperfurion; I/R, rats submitted to total ischemia of the hepatic pedicle for 25 min followed by 5 min of reperfusion; HBOI/R, rats submitted to 60 min of hyperbaric oxygen therapy at a pressure of 2 absolute atmospheres immediately after the experimental protocol of ischemia/reperfusion. Hepatic function was evaluated by quantitation of serum alanine aminotranferase (ALT) and aspartate aminotransferase (AST), and by mitochondrial function through the determination of states 3 and 4 of mitochondrial respiration, respiratory control ratio (RCR) and mitochondrial swelling. Data were analyzed by the Mann-Whitney test, with the level of significance set at p <0.05. RESULTS:There was a significant difference in state 3 values for the SHAM group vs I/R and I/R vs IRHBO, in state 4 values for the SHAM group vs I/R; and in mitochondrial swelling for the SHAM groups vs I/RHBO, SHAM vs I/R, and IR vs I/RHBO. CONCLUSION:The use of hyperbaric oxygen after I/R improved in a relative manner both the production of energy and the effects on the mitochondrial wall.

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Section: Discussioncontrasting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Effects of hyperbaric oxygen therapy on the liver after injury caused by the hepatic ischemia-reperfusion process

et al. 2014

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“…Oxygen 100% was used in all groups of HBO treatment. The chamber was gradually compressed and decompressed at 1.3 ATA/min for 15 minutes each, which was not considered as treatment time 7,13 .…”

Section: Hyperbaric Treatmentmentioning

confidence: 99%

“…The oxidative phosphorylation process is also severely affected by reperfusion, because of its location in the inner mitochondrial membrane, which is highly damaged by the oxygen reactive species. Therefore, damage caused to the membrane will result in a less efficient oxidative phosphorylation, such as occurs in the reperfusion process, because of an alteration in the membrane permeability and consequent mitochondrial swelling 7 . The effects of the I/R vary from alterations of hepatic enzymes to acute liver failure, depending on its duration 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Hyperoxic preconditioning in partial liver ischemia

et al. 2014

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PURPOSE:To evaluate the effect of the hyperbaric oxygen (HBO) treatment as a pre-conditioning for I/R effects in the liver ischemia. METHODS:Fifty-seven male Wistar rats (260-300g) were submitted to the following procedures: SHAM; I/R, rats submitted to I/R, consisting of partial ischemia of 70% of the liver for 90 minutes followed by 15 minutes of reperfusion; HBO I/R 1 ATA, 30 minutes of HBO treatment at the pressure of 1 absolute atmosphere (ATA) during the ischemia time. HBO I/R 2 ATA, 30 minutes of HBO (2 ATA) during the ischemia time. Pre HBO I/R 30', rats submitted to 30 minutes of HBO (2 ATA) immediately before the I/R time. Pre HBO I/R 90', rats submitted to 90 minutes of HBO (2 ATA) immediately before the I/R time. RESULTS:There was a significant worsening of all the parameters of mitochondrial energy production (state 3,4, RCR and Swelling) in the I/R group, when compared to the Sham group (I/R

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“…On this basis, a mitochondrial permeability transition occurs, characterized by a nonselective increase in the permeability of the inner mitochondrial membrane followed by osmotic swelling 5,[8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Effect of hyperbaric hepatic hyperoxia on the liver of rats submitted to intermittent ischemia/reperfusion injury

et al. 2014

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PURPOSE:To determine the effect of hyperbaric hyperoxia as hepatic preconditioning on hepatocellular integrity in rats submitted to intermittent hepatic ischemia/reperfusion injury. METHODS:Twenty male Wistar rats were divided into 4 groups (SHAM, I/R, HBO-I/R and CONTROL). The surgical technique consisted of total clamping of the hepatic pedicle for 15 min, followed by reperfusion for 5 min, performed twice. The application of hyperbaric oxygen (HBO) was carried out in a collective chamber (simultaneous exposure of 4 rats) pressurized directly with oxygen at 2 ATA for 60 min. Tissue malondialdehyde (MDA) levels were determined and blood samples were collected for the determination of serum AST and ALT levels. Data were analyzed statistically by the Mann-Whitney test, with the level of significance set at p < 0.05. RESULTS:A statistically significant difference in MDA (p< 0.05) was observed between control and HBO-I/R, but not between control and I/R. Regarding AST, there was a difference between control and I/R and HBO-I/R. Analysis of ALT revealed a significant difference between control and I/R (p<0.05) and between I/R and HBO-I/R, with no difference between control and HBO-IR. CONCLUSION:Hyperoxic preconditioning proved to be favorable regarding alanine transaminase, but not aspartate aminotranserase or malondialdehyde levels.Key words: Hyperbaric Oxygenation. Liver. Ischemia. Reperfusion. Lipid Peroxidation. Rats. Effect of hyperbaric hepatic hyperoxia on the liver of rats submitted to intermittent ischemia/reperfusion injury Acta

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Effects of hyperbaric oxygen (HBO), as pre-conditioning in liver of rats submitted to periodic liver ischemia/reperfusion

Abstract: In this process of periodic ischemia and reperfusion, hyperbaric pre-conditioning did not improve significantly hepatic mitochondrial function.

Cited by 11 publications

References 17 publications

Effects of hyperbaric oxygen therapy on the liver after injury caused by the hepatic ischemia-reperfusion process

Effects of hyperbaric oxygen therapy on the liver after injury caused by the hepatic ischemia-reperfusion process

Hyperoxic preconditioning in partial liver ischemia

Effect of hyperbaric hepatic hyperoxia on the liver of rats submitted to intermittent ischemia/reperfusion injury

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