Effect of probiotics on the development of dimethylhydrazine-induced preneoplastic lesions in the mice colon

Abstract

PURPOSE:To determine the effect of probiotics on the development of chemically induced (1, 2-dimethylhydrazine) colonic preneoplastic lesions, in mice. METHODS:The animals were divided into five groups. The control group was injected with carcinogen alone and the other groups also received probiotics (1-Lactobacillus delbrueckii UFV-H2b20; 2-Bifidobacterium animalis var. lactis Bb12; 3-L. delbrueckii UFVH2b20 plus B. animalis var. lactis Bb12; and 4-Saccharomyces boulardii) administered orally in drinking water throughout fourteen weeks. RESULTS:Consumption of lactobacilli and bifidobacteria alone resulted in a significant reduction of the total number of aberrant crypt foci (55.7% and 45.1%, respectively). Significant reduction in the number of these small foci (≤3 aberrant crypts) was only observed in the group treated with lactobacilli (52.2%) in comparison to control group. The number of larger foci (>3 aberrant crypts) crypts had no significant reduction. CONCLUSION:L. delbrueckii UFV-H2b20 and B. animalis var. lactis Bb12 administered alone protect colonic preneoplastic lesions in mice, while the combined treatment of these bacteria and the administration of S.boulardii were not effective in reducing such colonic lesions.

Germinated brown rice combined with Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis inhibits colorectal carcinogenesis in rats

“…Liboredo et al. () demonstrated that consumption of Lactobacillus delbrueckii UFVH2b20 resulted in a significant reduction in the number of colonic ACF, especially small ACF, in DMH‐induced mice. It supports the inhibitory activity of some Lactobacilli strains in early colorectal carcinogenesis as observed in our study.…”

The Role of Curcumin in Modulating Colonic Microbiota During Colitis and Colon Cancer Prevention

“…Similar effects may be ascribed to increased Bifidobacteria , which have also been shown to reduce aberrant crypt foci in mice receiving dimethylhyrazine. 61 Future experiments using gnotobiotic approaches would help establish the role of these microorganisms on colitis-associated CRC.…”

“…40,62 Analysis with the focus on the most abundant KEGG orthologs, which were affected by curcumin-supplementation in AOM/ Il10 −/− mice showed reduced abundance of functions involved in transport, transcription factors, bacterial chemotaxis and bacterial motility proteins. 56, 57, 61, 63–67 Curcumin has been observed to inhibit bacterial motility and cytotoxicity of Vibrio vulnificus 68 , to arrest Helicobacter pylori growth during infections 69 , as well as inhibit biofilm formation in Pseudomonas aeruginosa 70 . This suggests that the protective effect of 0.5% curcumin diet shifts the microbial composition to be more stationary, which may contribute to a decrease in microbial invasiveness and a decrease in overall disease severity.…”

275 The Role of Curcumin in Modulating Colonic Microbiota During Colitis and Colon Cancer Prevention

“…Similar effects may be ascribed to increased Bifidobacteria, which have also been shown to reduce aberrant crypt foci in mice receiving dimethylhyrazine. 61 Future experiments using gnotobiotic approaches would help establish the role of these microorganisms on colitisassociated CRC.…”

“…40,62 Analysis with the focus on the most abundant KEGG orthologs, which were affected by curcuminsupplementation in AOM/Il10 −/− mice showed reduced abundance of functions involved in transport, transcription factors, bacterial chemotaxis and bacterial motility proteins. 56,57,61,[63][64][65][66][67] Curcumin has been observed to inhibit bacterial motility and cytotoxicity of Vibrio vulnificus 68 , to arrest Helicobacter pylori growth during infections 69 , as well as inhibit biofilm formation in Pseudomonas aeruginosa 70 . This suggests that the protective effect of 0.5% curcumin diet shifts the microbial composition to be more stationary, which may contribute to a decrease in microbial invasiveness and a decrease in overall disease severity.…”