Aqueous-ozone irrigation of bone monocortical wounds in hyperglycemic rats

Abstract

PURPOSE:To analyze the effects of aqueous ozone irrigation over bone healing in hyperglycemia-induced rats. METHODS:Forty-eight male Wistar rats were allocated into Group H (hyperglycemic) or Group N (control). Monocortical bone wound were performed on femurs' anterolateral face. Wounds were treated with a trans-operatory single irrigation of 100ml of aqueous ozone [0.004mg/ml] whereas control groups received 100ml of pure water (Milli-Q ® ). Histomorphological and histomorphometrical analyses were accomplished after seven, 14 and 21 days. Kruskal-Wallis and Mann-Whitney statistical tests were applied for bone neoformation quantification and assessment. RESULTS:Aqueous ozone wounds irrigated revealed diffuse hemorrhage and increased neoformed of blood vessels number. There was no statistical significant difference in bone trabeculae neoformation. After seven and 14 days, the number of osteoclasts was higher in aqueous ozone groups than in those treated with pure water. CONCLUSION:Independently of blood glucose levels, aqueous ozone allowed an increase in blood vessels neoformation and osteoclast migration, without affect bone trabeculae neoformation.

Ozone therapy as an adjuvant for endondontic protocols: microbiological – ex vivo study and citotoxicity analyses

“…Such a concept justified the choice to employ the MTT methodology in the present study to evaluate the mitochondrial activity 11 , 17 . Previous literature has shown the effects of ozone to produce oxidative stress 1 , 2 , 1 , 4 , 8 , which in vitro reflects a immediate decrease in cell viability 1 , 4 confirmed by the cytotoxicity assay, decreasing cell viability from 100% to 98.2%, 77.3%, and 68.6% in Groups II, III, and IV, respectively. Free radicals are released to stimulate mitochondrion to produce adenosine triphosphate (ATP).…”

“…As a variation of oxygen, one of ozone’s greatest features is its oxidative power 2 , 4 , 19 . The oxidative stress, which is caused by its oxidative power, is responsible for the reactive oxygen species (ROS) which are converted into free radicals such as singlet oxygen, hydrogen peroxide, and superoxide anions 2 , 8 , 19 .…”

“…Ozone is both antimicrobial 2 , 6 , 7 , 10 , 12 - 18 and biocompatible 8 . Studies have shown the important efficacy of aqueous ozone 18 especially when associated with ultrasonic activation 10 , 17 .…”

“…In parallel, ozone increases the production of adenosine triphosphate (ATP) by mitochondria, which leads to a metabolic improvement and to healing of inflammatory/infectious processes 1 , 2 , 4 , 8 , 11 , 15 , 17 , 19 .…”

“…Furthermore due to the low level of hazardousness 11 , 17 and the high level of biocompatibility 8 , it might directly affect the healing process. Studies have proven its ability to interact effectively with microbiota in the root canal system and therefore to eliminate microorganisms 1 , 3 , 5 - 7 , 10 , 12 - 17 .…”