Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats

Almeida, Otavio Machado de; Jorgetti, Wanda; Oksman, Denis; Jorgetti, Camilo; Rocha, Dalva Marreiro; Gemperli, Rolf

doi:10.1590/s0102-86502013000100011

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…All the sponges presented a similar elliptical shape and a smooth, soft and sponge-like morphology along with thickness of 2 mm and wide length of ~7 mm. These uniform elliptical shapes can be ascribed to the 3 h refrigeration, freeze-drying and lyophilization [18]. Figure 2 showed the (a) FTIR spectra, (b) compressive strength, and (c) porosity of bare DC/HAp and 1, 5 and 10 μM EGCG/DC/HAp sponges.…”

Section: Discussionmentioning

confidence: 98%

Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration

Kook

Tian

Jeon

et al. 2017

Journal of Biomaterials Science, Polymer Edition

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Scaffolds mimicking structural and chemical characteristics of the native bone tissues are critical for bone tissue engineering. Herein, we have developed and characterized epigallocatechin gallate/duck's feet collagen/hydroxyapatite (EGCG/DC/HAp) composite sponges that enhanced the bone tissue regeneration. The three-dimensional composite sponges were synthesized by loading various amounts (i.e. 1, 5 and 10 μM) of EGCG to duck feet derived collagen followed by freeze-drying and then coating with hydroxyapatite. Several measuremental techniques were employed to examine the properties of the as-fabricated composite sponges including morphology and structure, porosity, compressive strength, etc. and as well compared with pristine duck feet derived collagen. SEM observations of EGCG/DC/HAp sponges showed the formation of a highly porous collagen matrix with EGCG embodiment. The porosity and pore size of sponges were found to increase by high EGCG content. The compressive strength was calculated as 3.54 ± 0.04, 3.63 ± 0.03, 3.89 ± 0.05, 4.047 ± 0.05 MPa for 1, 5 and 10 μM EGCG/DC/HAp sponges, respectively. Osteoblast-like cell (BMSCs isolated from rabbit) culture and in vivo experiments with EGCG/DC/HAp sponges implanted in nude mouse followed by histological staining showed enhanced cell internalization and attachment, cell proliferation, alkaline phosphatase expressions, indicating that EGCG/DC/HAp sponges have ahigh biocompatibility. Moreover, highEGCG content in the EGCG/DC/HAp sponges have led to increased cellular behavior. Collectively, the 5 μM of EGCG/DC/HAp sponges were suggested as the potential candidates for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 98%

Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration

Kook

Tian

Jeon

et al. 2017

Journal of Biomaterials Science, Polymer Edition

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“…Unlike ethylene oxide or GA sterilization, gamma radiation leaves no harmful residues that could potentially harm human health or the environment and is used to sterilize medical devices [6,22]. Moreover, gamma radiation-induced polymer crosslinking enables control of radiation-induced decomposition reactions, e.g., polymer chain scission, which can cause molecular weight reductions, as its effects are not dependent on material compositions [23][24][25]. Furthermore, when collagen is irradiated with gamma rays, peptide bonds are destroyed due to amino acid deformation, and hydrophilicity is improved by hydrogen bond formation [26].…”

Section: Introductionmentioning

confidence: 99%

Effects of Gamma Radiation-Induced Crosslinking of Collagen Type I Coated Dental Titanium Implants on Osseointegration and Bone Regeneration

et al. 2021

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This study aimed to compare two methods of crosslinking collagen type I on implanted titanium surfaces, that is, using glutaraldehyde (GA) or gamma-rays (GRs), in a beagle dog model. For in vivo experiments, implants were allocated to three groups and applied to mandibular bone defects in beagle dogs; Group SLA; non-treated Sandblasted, large grit, acid-etched (SLA) implants, Group GA; SLA implants coated with GA crosslinked collagen type I, Group GR; SLA surface implants coated with collagen type I and crosslinked using 25 kGy of 60Co gamma radiation. New bone μCT volumes were obtained, and histologic and histometric analyses were performed in regions of interest. The GR group had significantly better new bone areas (NBAs) and bone to implant contact (BIC) results than the SLA group (p < 0.05), but the GA and GR groups were similar in this respect. New bone volumes and inter-thread bone densities (ITBD) were non-significantly different in the three groups (p > 0.05). Within the limits of this study, gamma-ray collagen crosslinking on titanium implants can be considered a substitute for glutaraldehyde crosslinking.

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“…The objective of this study is to determine how the depletion of gonadal estrogen affects bone strength in young females. It is well known that estrogen stimulates and regulates pubertal bone growth, and thus is responsible for minimizing bone resorption and remodeling while promoting the thickening of the cortical long bone [4][5][6]. Therefore, females that have an estrogen deficiency or insensitivity (surgically induced or from a genetic disorder), demonstrate reduced bone mineral density, incomplete epiphyseal growth plate fusion, and an overall diminishment in skeletal integrity [4,5].…”

Section: Introductionmentioning

confidence: 99%

Using three-point bending to evaluate tibia bone strength in ovariectomized young mice

2017

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It is well known that estrogen deficiency induces a deterioration of bone strength in aged females. The aim of this study is to determine the effect of estrogen depletion on tibia bone strength in sexually mature mice that are still undergoing skeletal maturation. At 8 weeks of age, C57BL/6 female mice underwent an ovariectomy (OVX) or sham (SHAM) surgery. Mice were killed at 2, 4, or 8 weeks post-surgery. Tibia length and cross-sectional area continued to increase in both treatment groups until 4 weeks post-surgery. Compared to SHAM mice, OVX mice demonstrated a significant reduction in uterine weight and plasma estrogen levels. Three-point bending was used to quantify the mechanical properties (breaking point, stress, stiffness, and elasticity) of the tibia. The tibias from the SHAM mice had a higher breaking point than all the age-matched OVX mice. At 8 weeks post-surgery, the tibias from the SHAM mice demonstrated higher elasticity, stress, and stiffness than the younger SHAM mice and the age-matched OVX mice. Compared to the SHAM mice, our study suggests that (1) there is a reduction in the mechanical strength of tibias from young OVX mice, and (2) the greatest decline in tibia strength of the OVX mice was once they reached skeletal maturity.

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Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats

Cited by 6 publications

References 11 publications

Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration

Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration

Effects of Gamma Radiation-Induced Crosslinking of Collagen Type I Coated Dental Titanium Implants on Osseointegration and Bone Regeneration

Using three-point bending to evaluate tibia bone strength in ovariectomized young mice

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