Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury

Abstract

PURPOSE: To determine the profile of gene expressions associated with oxidative stress and thereby contribute to establish parameters about the role of enzyme clusters related to the ischemia/reperfusion intestinal injury. METHODS: Twelve male inbred mice (C57BL/6) were randomly assigned: Control Group (CG) submitted to anesthesia, laparotomy and observed by 120min; Ischemia/reperfusion Group (IRG) submitted to anesthesia, laparotomy, 60min of small bowel ischemia and 60min of reperfusion. A pool of six samples was submitted to the qPCR-RT protocol (six clusters) for mouse oxidative stress and antioxidant defense pathways. RESULTS: On the 84 genes investigated, 64 (76.2%) had statistic significant expression and 20 (23.8%) showed no statistical difference to the control group. From these 64 significantly expressed genes, 60 (93.7%) were up-regulated and 04 (6.3%) were down-regulated. From the group with no statistical significantly expression, 12 genes were up-regulated and 8 genes were down-regulated. Surprisingly, 37 (44.04%) showed a higher than threefold up-regulation and then arbitrarily the values was considered as a very significant. Thus, 37 genes (44.04%) were expressed very significantly up-regulated. The remained 47 (55.9%) genes were up-regulated less than three folds (35 genes - 41.6%) or down-regulated less than three folds (12 genes - 14.3%). CONCLUSION: The intestinal ischemia and reperfusion promote a global hyper-expression profile of six different clusters genes related to antioxidant defense and oxidative stress.

Propofol Attenuates Small Intestinal Ischemia Reperfusion Injury through Inhibiting NADPH Oxidase Mediated Mast Cell Activation

“…During IIR, oxidative stress is increased due to burst production of reactive oxygen species (ROS), which is a major mechanism of IIR injury [ 5 ]. Numerous studies have revealed that increased ROS production resulted from overactivation of the prooxidant enzyme NADPH oxidase, which abundantly exists in intestine tissue [ 6 ] and plays critical roles in mediating tissue injury related to a range of inflammatory diseases [ 7 ], including ischemia reperfusion injury [ 8 , 9 ].…”

The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline

“…NO in such conditions may promote tissue damage or beneficial effects 2,4,5,9,11,14,15,25,28 . Analyses of the expression levels of 84 genes encoding proteins that are associated with oxidative stress during 120 minutes of IR in mouse intestine indicated 47 genes, including NOS-2, showed increased expression 29 . Previously, mRNA expression levels of NOS-2 and NOS-3 were evaluated during IR of the superior mesenteric artery (40 minutes of ischemia and 300 minutes of reperfusion) in the rat intestine.…”

Effect of hyperbaric oxygenation on the expression of glutathione peroxidase 4 and lactoperoxidase genes in the lung of isogenic mice after ischemia/reperfusion injury in the small bowel

“…Due to their high sensitivity, specificity and reproducibility, genomic approaches using molecular biology techniques permit the reliable evaluation of the expression levels of a large number of genes. This is relatively simplified by the use of reverse transcription and quantitative polymerase chain reaction (RT-qPCR) in real time [6][7][8] . This technique permits study of the expression of genes involved in oxidative stress and antioxidant defense both Effect of hyperbaric oxygenation on the expression of glutathione peroxidase 4 and lactoperoxidase genes in the lung of isogenic mice after ischemia/reperfusion injury in the small bowel Ikejiri AT et al…”

“…Acta Cir Bras. 2018;33(5):462-471 in local organs 8 and in organs distant from ischemia 9,10 , allowing a better understanding of the various aspects of this phenomenon in the diagnosis, monitoring and treatment of ischemic disease.…”