0.1T magnetic resonance image in the study of experimental hydrocephalus in rats. Accuracy of the method in the measurements of the ventricular size

Abstract

PURPOSE: To investigate the accuracy of 1.0T Magnetic Resonance Imaging (MRI) to measure the ventricular size in experimental hydrocephalus in pup rats. METHODS: Wistar rats were subjected to hydrocephalus by intracisternal injection of 20% kaolin (n=13). Ten rats remained uninjected to be used as controls. At the endpoint of experiment animals were submitted to MRI of brain and killed. The ventricular size was assessed using three measures: ventricular ratio (VR), the cortical thickness (Cx) and the ventricles area (VA), performed on photographs of anatomical sections and MRI. RESULTS: The images obtained through MR present enough quality to show the lateral ventricular cavities but not to demonstrate the difference between the cortex and the white matter, as well as the details of the deep structures of the brain. There were no statistically differences between the measures on anatomical sections and MRI of VR and Cx (p=0.9946 and p=0.5992, respectively). There was difference between VA measured on anatomical sections and MRI (p<0.0001). CONCLUSION: The parameters obtained through 1.0T MRI were sufficient in quality to individualize the ventricular cavities and the cerebral cortex, and to calculate the ventricular ratio in hydrocephalus rats when compared to their respective anatomic slice.

Pre- and Postshunting Magnetization Transfer Ratios Are in Accordance with Neurological and Behavioral Changes in Hydrocephalic Immature Rats

“…Different MRI techniques have been used in hydrocephalus conditions to quantify myelin and the concentration of metabolites resulting from tissue degradation [37,38,39,40,41,42,43]. Among these techniques, an important tool to assess the damage to nervous tissue through the identification and quantification of axonal myelination processes is the MTR [22].…”

Análise dos efeitos clínico-radiológicos, histopatológicos e bioquímicos da derivação ventrículo-subcutânea na hidrocefalia induzida por caulim em ratos

“…Surpreendentemente, considerando o número crescente de estudos relacionados à hidrocefalia experimental nos últimos anos, há poucas descrições publicadas de derivações experimentais (MILLER et al, 2007;ESKANDARI et al, 2012;CASTRO et al, 2012;CATALÃO et al, 2014). A técnica utilizada neste trabalho envolveu uma derivação entre os ventrículos laterais do animal e o espaço subcutâneo criado próximo a sua cauda, que se mostrou simples e confiável (SANTOS et al, 2016).…”

Análise dos efeitos da oxigenoterapia hiperbárica associada ao tratamento cirúrgico em lesões cerebrais secundárias à hidrocefalia experimental

“…Nosso grupo de pesquisa vem trabalhando incessantemente há mais de 10 anos no estudo de diversas drogas e procedimentos que possam oferecer efeitos neuroprotetores na hidrocefalia experimental induzida por caulim (CATALÃO et al, 2014;CATALÃO et al, 2017;DA SILVA et al, 2018;GARCIA et al, 2017;SAMPAIO et al, 2019;TIDA et al, 2018), no mapeamento de lesões secundárias à ventriculomegalia (AISENGART et al, 2011;CATALÃO et al, 2019;DEL BIGIO et al, 2012;LACHAT, 2003;TAVEIRA et al, 2012), em ensaios sobre o curso do tratamento cirúrgico experimental (ROCHA CATALÃO et al, 2014;SANTOS et al, 2016;SANTOS et al, 2019) e, também, na análise de exames de imagem complementares que delimitam a melhor visualização das estruturas cerebrais e auxiliam no diagnóstico da hidrocefalia experimental (CASTRO et al, 2012;DE MOURA SILVA et al, 2021;LUCCAS;SAMPAIO et al, 2015). Além destes, podemos incluir nesta lista estudos em fase de preparação/publicação utilizando drogas como o Resveratrol, a Memantina, Canabidiol, Celecoxibe, entre outros.…”