Conjunctival inflammation in patients under topical glaucoma treatment with indication to surgery

Abstract

PURPOSE:To compare the frequency of conjunctival HLA-DR expression (a surrogate marker for inflammation) in eyes treated with topical prostaglandin analogues versus eyes treated with other topical antiglaucomatous drugs. METHODS: Patients diagnosed with primary open-angle glaucoma presenting indication for trabeculectomy were divided in groups according to the use or not of prostaglandin analogues. All subjects were treated with the maximum tolerated dose of antiglaucomatous drugs until the date of the surgery. At the beginning of the surgical procedure, a 5 x 5 mm biopsy of the bulbar conjunctiva was collected, incubated with monoclonal anti-HLA-DR antibody and processed for histological analysis. RESULTS: Of the 31 eyes included (31 patients), 25 were under topical prostaglandin analogues (Group 1) and six under other topical pharmacological agents (Group 2). Fourteen eyes of Group 1 (56%) and three of Group 2 (50 %) were positive for the inflammatory marker HLA-DR (P=1.0). The percentage of stained cells ranged from 15.49 to 48.09% (median: 27.61) in Group 1, and from 18.35 to 28 (median: 20.71) in Group 2, with no differences statistically significant (p=0.33). CONCLUSION: The use of prostaglandin analogues did not increase conjunctival expression of HLA-DR compared to other topical antiglaucomatous agents.

Pathobiology of wound healing after glaucoma filtration surgery

“…Another problem making management of glaucoma somewhat problematic is that a commonly prescribed drug class, prostaglandin analogues, can induce inflammation by upregulating proinflammatory cytokine gene expression even though they stably reduce intraocular pressure [ 11 , 12 ]. There are several reports describing an inverse correlation between subclinical inflammation and filtration surgery outcome.…”

Puerarin regulates neovascular glaucoma through pigment epithelium‑derived growth factor‑induced NF‑κB signaling pathway

“…Chang et al (34) suggested increasing mast cell numbers as a possible indicator of preoperative glaucoma surgery inflammation was observed in the conjunctiva of glaucoma patients. In addition, Furtado et al (35) have showed that inflammation in patients under topical glaucoma is an indicator following treatment with indication to surgery. Vohra et al (36) have indicated that the role of inflammation in the pathogenesis of glaucoma.…”

Influência da prostaglandina F>sub<2&#945;>/sub< sobre alguns aspectos imunorregulatórios das células-tronco mesenquimais

“…Esses resultados são corroborados pela produção reduzida de IL1β, que é uma citocina pró-inflamatória bem conhecida (Ebrahim et al, 2018). Alguns estudos também revelaram efeitos anti-inflamatórios da PGF2α em outros tipos e modelos de células: suprimindo a permeabilidade vascular em um modelo induzido por doença crônica de granuloma; sendo capaz de reduzir a expressão lútea de TNF-α e também não aumentando a expressão conjuntival de HLA-DR (marcador inflamatório) (Furtado et al, 2012;Hernandez et al, 2009;Ohuchi et al, 1977). Além disso, nosso modelo também mostrou uma produção reduzida do fator estimulador de colônias de granulócitos/ macrófagos (GM-CSF).…”