Effects of chitosan on healing and strength of colonic anastomosis in rats

Çıtgez, Bülent; Cengiz, Ahmet Nurettin; Akgün, İsmail; Uludağ, Mehmet; Yetkın, Gürkan; Bahat, Neslihan; Özcan, Ömer; Polat, Nedim; Akçakaya, Adem; Karatepe, Oğuzhan

doi:10.1590/s0102-86502012001000007

Cited by 12 publications

(12 citation statements)

References 35 publications

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“…There was no significant difference for collagen type III between catgut and control groups; however, for the chitosan, there was a significant increase in collagen type III, which suggests a superior quality in the tissue repair process. These results corroborate the effects of chitosan powder on anastomosis healing in rat colons that resulted in a significant increase of collagen deposition, and thus, healing improvement . In the same way, Ueno et al showed an increase in collagen type III deposition with the use of chitosan‐based topical formulations for injury healing.…”

Section: Discussionsupporting

confidence: 79%

“…These results corroborate the effects of chitosan powder on anastomosis healing in rat colons that resulted in a significant increase of collagen deposition, and thus, healing improvement. 21 In the same way, Ueno et al 22 showed an increase in collagen type III deposition with the use of chitosan-based topical formulations for injury healing.…”

Section: Discussionmentioning

confidence: 91%

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Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

et al. 2015

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The objective of the current article was to compare the quality of the healing process after hysterorrhaphy with catgut and chitosan suture wires via macroscopic, histochemical, and immunohistochemical evaluations. Seven ewes were submitted to a video-assisted laparotomy. A hysterectomy was performed with subsequent catgut and chitosan hysterorrhaphy in both horns. Then, macroscopic evaluation and biopsies of the hysterorrhaphy and control areas were carried out after 30 days. Although postoperative adhesion was observed in five animals of the catgut group, no adhesions were detected in the chitosan one. Additionally, there was a significant difference in increasing collagen type III and connective tissue for the chitosan group when compared to the control and catgut groups. A larger amount of blood vessels was also observed in the control group in comparison with the catgut and chitosan groups. Collagen type I and vascular endothelial growth factor-A (VEGF-A) were significantly different among groups. Thus, chitosan promoted a preventive effect on postoperative adhesion formation in the hysterorrhaphy areas, with increasing collagen type III deposition during tissue mending, which granted an enhanced healing process.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 91%

Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

et al. 2015

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“…The high biodegradability properties reported in our previous experiments and this study, are probably due to colonic microflora and enzymatic activity . In experimental in vivo study, chitosan powder applied to colonic anastomosis had a positive effect in the process of collagenization in colonic anastomosis healing and composite collagen/chitosan scaffold appeared to be suitable to support in vitro bioengineered smooth muscle intestinal constructs . Tissue engineering of the colorectal tissue involves the regeneration of the smooth muscle layer.…”

Section: Discussionsupporting

confidence: 59%

Colorectal wall regeneration resulting from the association of chitosan hydrogel and stromal vascular fraction from adipose tissue

Denost

Pontallier

Buscail

et al. 2017

J Biomedical Materials Res

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Chitosan hydrogel and adipose derived stem cells (ADCS) have been reported as the optimal partnership for colorectal tissue engineering. In that field, the aim of the current experiment was to assess the interest of seeding ADSC on chitosan hydrogel patches in an in vivo comparative study and on a tube intended replace a colonic segment in an in vivo feasibility study. In the comparative study, a 2 × 3 cm colonic wall defect was performed in 20 swine and repaired by suturing a chitosan hydrogel patch: acellular matrix (group A, n = 10) versus matrix seeded with autologous stromal vascular fraction (SVF) (group B, n = 10). In the feasibility study, a circular colonic section was performed and a 2-cm-length chitosan hydrogel tube (seeded with autologous SVF) was implanted between the two edges of the colon in 3 pigs. Graft areas were explanted at 8 weeks for examinations. Endpoints were technical feasibility, fibrosis ratio, and smooth muscle layer regeneration. A complete coverage of the patched area was observed with an ad integrum regeneration of the colonic wall including smooth muscle cells layer around a thin fibrosis scare. Fibrosis ratio was significantly lower group B: 13% versus 55% (p = 0.013). Segmental colonic replacement appeared accurate. Our data confirmed in a large animal model the healing effect of chitosan on colorectal tissue. The very low rate of the fibrosis ratio in the cellularized group emphasizes inflammatory control effect of the chitosan hydrogel and SVF association. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 460-467, 2018.

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“…In addition, previous work has shown that diff erent molecular weights of β-Chitosane have a range of antiinfl ammatory actions, including inhibition of production of TNF-α, IL-6, prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), VCAM-1 and ICAM-1 in vitro [46]. Also, a decrease in oxidative tissue lesions, septic lesions, organ lesions, and cytokine levels mediated by this biomolecule was observed [47,48].…”

Section: Microscopic Observations (Histological Study)mentioning

confidence: 97%

Beta-Chitosane as a Treatment for Ulcerative Colitis: Therapeutic Effectiveness and Possible Mechanisms of Action

Laribi-Habchi¹,

Yasmine²,

Zineb³

et al. 2021

J Biomed Res Environ Sci

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Purpose: Colitis is a widespread inflammatory bowel disease with heterogeneous etiology (genetic and immunological). It is treated with drugs such as steroidal and non-steroidal anti-inflammatory that, in the long term, can cause side effects. For this reason, the exploitation of natural resources to combat this type of disease is the concern of researchers. The purpose of our study was to evaluate the anti-colitis (anti-inflammatory) effect of β-Chitosane induced in albino mice by acetic acid (5%). Methods: Mices were separated into six groups: the witness (untreated and not ulcerated), negative control group (ulcerated and untreated), the positive control ulcerated and treated with the Dexaméthasone® (1 mg/kg), and test groups ulcerated and treated with different doses of β-Chitosane (0.5 g/ kg; 0.75 g/ kg and 1 g/ kg) for the entire treatment estimated to six-days. β-Chitosane efficacy was evaluated by macroscopic and microscopic scores. Results: The clinical scores showed that β-Chitosane with a dose of 1 g/kg for the entire treatment significantly reduced the damage caused by acetic acid with a score of (3.41 ± 1.45) compared to those of the positive control which reduced less the inflammation (6.26 ± 1.23). The histological study of the colons was able to validate the effect of β-Chitosane by decreasing neutrophil infiltration and ulceration in the colon as well as by structural recovery of the mucosa. Conclusion: These results provide evidence that β-Chitosane has a protective effect against ulcerative colitis that may be due to its antioxidant, anti infectious, anti-inflammatory and healing activities.

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Effects of chitosan on healing and strength of colonic anastomosis in rats

Cited by 12 publications

References 35 publications

Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

Colorectal wall regeneration resulting from the association of chitosan hydrogel and stromal vascular fraction from adipose tissue

Beta-Chitosane as a Treatment for Ulcerative Colitis: Therapeutic Effectiveness and Possible Mechanisms of Action

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