Anti toxic effect of broccoli extract on stannous dichloride toxicity

Abstract

PURPOSE: Since Technetium-99m ( 99m Tc) has favorable physical and chemical characteristics, it is widely used radioisotope in Nuclear Medicine. However, stannous dichloride (SnCl 2 ) has been widely used as a reducing agent in labeling procedure of pharmaceutical with radionuclide, it has been realized that SnCl 2 have genotoxic and cytotoxic effects on biological systems. In previous studies, it has been shown that some herbal extract can reduce genotoxic and cytotoxic effects of SnCl 2 . In the present study, it is aimed to evaluate the effect of the broccoli extract on the survival of E. coli ATCC 25922 strain against to toxic effects of SnCl 2 . METHODS:Broccoli was extracted with methanol extraction. HPLC and TLC analysis of broccoli extract were performed. Then antitoxicity and dose response assays were performed on bacterial strain. RESULTS:The broccoli extract had dose dependent protective effect against SnCl 2 toxic effect on E. coli. CONCLUSIONS:The consumption of broccoli may alter the stannous dichloride toxicity. Broccoli extract may use as a new protective strategies against the toxic effect of SnCl 2 on patients who were taken 99m Tc radiopharmaceuticals. Key words: Broccoli. Stannous dichloride. Technetium-99m. Escherichia coli. Toxicity. RESUMOOBJETIVO: Em face de suas características físico-químicas, o Tecnécio-99m ( 99m Tc) é um radiofármaco amplamente utilizado na Medicina Nuclear. Todavia, o dicloreto de estanho (SnCl 2 ) tem sido largamente aplicado como um agente redutor no procedimento farmacêutico de marcação com radionuclídeos. Constatou-se que o SnCl 2 apresenta efeitos genotóxicos e citotóxicos nos sistemas biológicos. Em estudos prévios, foi demonstrado que alguns extratos de ervas podem reduzir tais efeitos. O estudo atual objetivou avaliar os efeitos do extrato de brócolis na sobrevida da cepa E. coli ATCC 25922, exposta ao efeito tóxico do SnCl 2.MÉTODOS: O extrato de brócolis foi obtido mediante extração com metanol. Analises com HPLC e TLC foram efetuadas. Avaliou-se a antitoxicidade e realizou-se um ensaio dose-resposta para uma cepa de bactérias. RESULTADOS:O extrato de brócolis mostrou um efeito protetor dose dependente para os efeitos tóxicos do SnCl 2 sobre a E. coli. CONCLUSÕES:O consumo de brócolis pode alterar a toxicidade do dicloreto de estanho . O extrato de brócolis pode ser utilizado como uma nova estratégia para proteção de pacientes contra os efeitos tóxicos do SnCl 2, nos quais foi administrado o radiofármaco Tecnécio-99m.Descritores: Brassica. Compostos Estanho. Tecnécio. Escherichia coli. Toxicidade. Anti toxic effect of broccoli extract on stannous dichloride toxicity

Phyto-Niosomes:In VitroAssessment of the Novel Nanovesicles Containing Marigold Extract

“…In addition to their dietary properties, many medicinal plants can be promising sources of natural products with beneficial activity in human and animal health [2]. In vitro and in vivo investigations have been performed to evaluate their biological activities, biodistribution and toxicity [3][4][5]. The antioxidant and antimicrobial activity of different extracts obtained from several edible or non-edible, medicinal plants, including their leaves, bark and roots [6,7], flower, fruits and seeds [8], resins and tars [9,10] and seedcakes have been comprehensively studied.…”