Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Abstract

PuRPOSE:To evaluate the effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis. METHOdS: Forty rats were divided into two groups to receive either bromopride (experimental group-E) or saline (control group-C). Each group was divided into subgroups of ten animals each to be euthanized on third (E3 and C3) or seventh day (E7 and C7) after surgery. Sepsis was induced by cecal ligation and puncture. The rats underwent segmental left colon resection and end-to-end anastomosis. Adhesion formation, tensile strength and hydroxyproline concentration were assessed. Histomorphometry of collagen and histopathological analysis were also performed. RESuLTS: On postoperative third day, anastomoses in bromopride-treated animals showed lower tensile strength (p=0.02) and greater reduction in hydroxyproline concentration (p=0.04) than in control animals. There was no statistical difference in these parameters on seventh day, and the remaining parameters were similar across subgroups. Collagen content was also similar across subgroups. CONCLuSION: In the presence of abdominal sepsis, the administration of bromopride was associated with decreased tensile strength and hydroxyproline concentration in left colonic anastomoses in rats three days after surgery. Key words: Anastomosis, Surgical. Colon. Sepsis. Grastrointestinal Motility. Tissue Adhesions. Rats. RESuMO OBJETIVO:Avaliar os efeitos da bromoprida sobre a cicatrização de anastomoses de cólon esquerdo de ratos na presença de sepse abdominal. MéTOdOS: Quarenta ratos distribuídos em grupos contendo 20 animais para administração de bromoprida ou salina. Cada grupo foi dividido em subgrupos contendo dez animais, para eutanásia no terceiro ou no sétimo dia de pós-operatório. A indução da sepse foi realizada pelo método de ligadura e punção do ceco. Foi realizada ressecção de um segmento do cólon esquerdo e anastomose término-terminal. À re-laparotomia, foi avaliada a quantidade total de aderências e removido um segmento colônico contendo a anastomose para análise histopatológica, força de ruptura, concentração de hidroxiprolina e histomorfometria do colágeno. RESuLTAdOS: No 3° DPO, as anastomoses dos animais tratados com bromoprida apresentaram menor força de ruptura (p=0,02) e maior redução da concentração de hidroxiprolina (p=0,04) que os animais controle. Não houve diferença estatística quanto a estes parâmetros no 7° DPO. O conteúdo de colágeno foi semelhante entre os subgrupos. CONCLuSãO: Na presença de sepse abdominal, o uso da bromoprida esteve associado à diminuição da força de ruptura e da concentração de hidroxiprolina das anastomoses do cólon esquerdo de ratos no 3° DPO. descritores: Anastomose Cirúrgica. Cólon. Sepse. Motilidade Gastrointestinal. Aderências Teciduais. Ratos. Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Effects of bromopride on expression of metalloproteinases and interleukins in left colonic anastomoses: an experimental study

“…Conversely, in previous studies we found that administration of bromopride in the presence or absence of sepsis was associated with decreased tensile strength of left colonic anastomoses in rats 3 days after surgery, with no changes in adhesion formation ( 9 , 10 ), suggesting that other mechanisms may be involved. Therefore, the study of other healing parameters to elucidate the real action of bromopride on the healing process is needed.…”

Dopamine receptors in emesis: Molecular mechanisms and potential therapeutic function

“…-Effective treatment for preventing postoperative nausea and vomiting as well as chemotherapyinduced emesis [336]. -Was associated with decreased tensile strength and hydroxyproline concentration in colonic anastomoses in rats three days after surgery [337].…”