Propofol effects on the morphology of rat testes subjected to testicular ischemiareperfusion

Abstract

PURPOSE:To evaluate the effects of propofol as an inhibitor of tissue injury in testicular ischemia-reperfusion in rats. METHODS:30 Wistar rats were assigned to one of three groups of 10 animals: G1, testicular exposure alone; G2 and G3: testicular ischemia caused by left spermatic cord torsion of 720º. In G3, propofol was administered intraperitoneally at 20 mg/kg/h 45 minutes after spermatic cord torsion. In G2 and G3, spermatic cords were detorsioned after 60 min. In all three groups, testes were subsequently repositioned in the scrotum. After 90 days, bilateral orchiectomy was performed for histological examination. RESULTS:No abnormalities in seminiferous tubules were found in G1. In G2, 86.6% of left testes exhibited abnormalities, in contrast with 67.8% for right testes. In G3, these proportions were 57.3% and 45.6%, respectively. A statistically significant difference was found between G2 and G3. CONCLUSION: Propofol reduced the tissue damage in rat testes subjected to ischemia-reperfusion caused by spermatic cord torsion. Key words: Propofol. Testis. Ischemia. Reperfusion. Rats. RESUMO OBJETIVO:Avaliar os efeitos do propofol como inibidor da lesão tecidual na isquemia-reperfusão testicular em ratos. MÉTODOS:Trinta ratos Wistar foram distribuídos em três grupos de 10 animais. G1: apenas exposição testicular. G2 e G3: isquemia testicular por torção do cordão espermático esquerdo a 720º. G3, 45 minutos após a torção do cordão espermático foi administrado propofol 20mg/Kg/hora via intraperitoneal. Após 60 minutos, nos grupos 2 e 3 foram desfeitas as torções dos cordões espermáticos e em seguida os testículos dos animais dos três grupos foram reposicionados no escroto. Após 90 dias foi realizada a orquiectomia bilateral para análise histológica. RESULTADOS:Nos túbulos seminíferos do grupo 1 não se encontrou anormalidades. Nos túbulos seminíferos do Grupo 2, as anormalidades foram 86,6% nos testículos esquerdos e 67,8% nos testículos direitos. Houve diferença estatisticamente significativa quando se compararam os testículos dos grupos 2 e 3. CONCLUSÃO:O propofol minimiza a lesão tecidual em testículos de ratos submetidos à isquemia-reperfusão na torção do cordão espermático.Descritores: Propofol. Testículos. Isquemia. Reperfusão. Ratos. Propofol effects on the morphology of rat testes subjected to testicular ischemia-reperfusion

Protective effects of thymoquinone on experimental testicular ischaemia-reperfusion injury: an apoptotic, proliferative and biochemical study

“…Nitric oxide synthesis inhibitors and ROS cleaners were shown to prevent ischaemiareperfusion damage arising in different tissues (Filho et al, 2004). Similarly, resveratrol, epigallocatechin and propofol applications have been reported to be useful in preventing ischaemia-reperfusion damage (Al-Maghrebi et al, 2012;Urt Filho et al, 2012;Yulug et al, 2014). Yulug et al (2014) emphasised in their studies in 2014 that resveratrol decreases tissue damage in testis torsion that has continued for 4 h. Similarly, the application of interleukin 10 was reported to protect testicular tissue against detorsion damage (Ozturk et al, 2014).…”

Twisting of the spermatic cord: ischemia and reperfusion, toxicogenetic evaluation, and the effects of phosphatidylcholine in pre-clinical trials

“…Mean values ± SE of histological classes of damage and score. *According to Consentino et al (1985) and Urt Filho et al (2012) …”

“…The samples were cut on a microtome at a thickness of 5 mm, stained with hematoxylin and eosin (H&E), and examined under a bright field microscope at a magnification of 1000X (model Eclipse e-200, Nikon ® ). Histopathological evaluation was performed by a double-blind model according to the damage scale proposed by Cosentino et al (1985) and modified by Urt Filho et al (2012). Damage was classified as: grade 1, normal testicular architecture and orderly arrangement of germ cells; grade 2, lower histological organization, loss of cohesion between germ cells, and closed seminiferous tubules; grade 3, higher disorganization of testicular architecture, germ cells with pyknotic and retracted nuclei, and seminiferous tubules with poorly defined edges; and grade 4, presence of occluded seminiferous tubules and coagulative necrosis of germ cells.…”

Medical perspective in testicular ischemia-reperfusion injury

“…Propofol seems to have antioxidant effects that inhibit lipid peroxidation by forming relatively unreactive free radicals capable of breaking the production chain of radicals that are potentially damaging to cell membranes in tIR injury (27).…”