Histology of the rectus abdominis muscle in rats subjected to cranial and caudal devascularization

Abstract

<jats:p>PURPOSE: To investigate the microscopic changes in the rectus abdominis muscle in rats subjected to five delay procedure. METHODS: 30 male holtzmann rats, weighting between 250 and 350 grams, were used. The animals were divided into five groups (n=6): A - cranial section of the right muscle; B - caudal section of the right muscle; C - craniocaudal section of the right muscle; D - cranial section reflecting the right muscle in the craniocaudal direction; E - caudal section reflecting the right muscle in the caudocranial direction. On the seventh day after surgery, a resection of the cranial and caudal fragments of the right and left muscles, respectively, was performed for microscopic analysis. Histological alterations were quantified and the right and left (control) muscle fragments compared. Fisher's exact test was used for statistical purposes with a significance level of 5%. RESULTS: The comparison between right and left muscles showed statistically significant differences in group A - inflammatory infiltrate in the cranial fragment (p=0.015); in group C - inflammatory infiltrate (p=0.000) and necrosis (p=0.015) in the caudal fragment; and in group E - edema in the caudal fragment (p=0.000). No significant alterations were noted in groups B and D. CONCLUSIONS: Irrigation exclusively through the perforating muscle vessels is inappropriate; irrigation exclusively through the cranial pedicle causes milder, insignificant histological alterations, when compared with irrigation exclusively through the caudal pedicle; irrigation exclusively through the caudal pedicle causes more pronounced, but still insignificant, histological alterations when compared with other forms of devascularization.</jats:p>

Comparison of the Effects of Inhalation and Spinal Anesthesia on Microcirculation in Transverse Rectus Abdominis and Gluteus Maximus Muscle-Skin Flap Applications in Experimental Rat Models

“…The effects of general anesthetics on cardiac output, arterial blood pressure and regional blood flow have been documented, while the effects of spinal anesthesia on microcirculation in the transferred tissue have yet to be established experimentally (Costa et al, 2012;Schusterman et al, 1992;Carden et al, 2000). The present study evaluates the effect of anesthetic techniques on flap microcirculation, and thus it contributes to literature.…”

“…Flaps are commonly used in plastic and reconstructive surgery for the repair of tissue defects occurring due to various etiologies, such as trauma or tumor excision (Nahai et al, 1984;Taylor et al, 1994;Özdemir et al, 2017;Mathes et al, 1977;Costa et al, 2012). In flap surgeries, the success of operation is dependent upon the experience of surgeon, as well as technical factors.…”

Use of a semitendinosus myocutaneous flap for the coverage of hindlimb full-thickness skin defects in cats

“…By day 14, significantly higher scores were recorded in all parameters than on day zero. These scores probably represent the effects of the inflammatory response during wound healing, compromised blood flow, and the reperfusion injury that the ST flap normally developed 2 wk postoperatively at its distal part, where the sample points were harvested [ 28 29 30 31 ]. The 6- and 12-mon examination samples were characterized mostly by muscle cell degeneration ( Fig.…”

“…All samples were processed using standard techniques and stained with haematoxylin and eosin. The stained sections were evaluated to assess inflammation, muscle cell degeneration, muscle cell necrosis, neovascularization, fibrosis, and edema quantitatively classified as follows: 0 (normal), no alterations present; 1 (+), alterations up to 33%; 2 (++), alterations between 34% and 66%; 3 (+++), alterations between 67% and 100% [ 28 29 30 31 ].…”

Διερεύνηση Της Χρήσης Μυοδερματικών Κρημνών Στα Οπίσθια Άκρα Της Γάτας

“…Όλα τα ιστολογικά παρασκευάσματα εξετάστηκαν με τη χρήση οπτικού μικροσκοπίου από το ίδιο άτομο και χωρίς ο παθολογοανατόμος να γνωρίζει τις ομάδες προέλευσής τους (τυφλή εξέταση). Οι παράμετροι που εκτιμήθηκαν και ποσοτικοποιήθηκαν ήταν: η φλεγμονή, το οίδημα, η ίνωση, η αγγειογένεση, η εκφύλιση και η νέκρωση των μυϊκών ινών (Costa 2012, Degner 1996, Greaves 1996, Siemionow 1995, Zellner 2015. Η βαθμολόγηση των παραμέτρων έγινε ως εξής: 1) 0 (φυσιολογικό), όταν δεν υπήρχαν μεταβολές, 2) 1 (+) μεταβολές πάνω από 33%, 3) 2 (++) μεταβολές μεταξύ 34 και 66% και 4) 3 (+++) μεταβολές μεταξύ 67 και 100%.…”