Comparative study of the areas of osteochondral defects produced in the femoral condyles of rabbits treated with gel of sugarcane biopolymer

Abstract

PURPOSE: To measure the healed areas of osteochondral defects produced in femoral condyles of rabbits filled with biopolymer sugar cane gel and to compare these with those of the control group at 90, 120 and 180 days. METHODS: A study was made of 16 New Zealand rabbits, 6 and 7 months old, weighing between 2 and 2.5 kg. Defects of 3.2 x 4 mm were made, with trephine, in the femoral condyles of the right and left knees. As to the study group defects of the medial and lateral condyles of the right knee were used which were filled with Biopolymer Sugar Cane Gel; as to the Control Group defects of the medial and lateral condyles of the left t knees were used which were left open for natural healing. The defects were analyzed at 90, 120 and 180 days after surgery. After euthanasia, the knees were removed and fixed in Bouin's solution for later digital photographic documentation with a digital camera. The areas healed were measured in both the study and control groups using the images obtained from an Image-J® program. Statistical analysis was conducted using the non-parametric Mann-Whitney test. RESULTS: There were no significant differences between the means of the healed areas in the study and control groups at 90, 120 and 180 days after surgery. CONCLUSION: The dimension of the healed areas of the defects treated with the biopolymer sugar-cane gel in the study group was similar to those of the control group, which healed naturally.

Cartilage Repair Using Hydrogels: A Critical Review of in Vivo Experimental Designs

“…69,70 Among the literature revised, 39% of studies proposed natural materials including collag e n , 2 1 , 2 2 , 2 5 , 2 6 , 2 8 , 3 2 , 3 9 , 4 7 , 5 0 , 5 2 , 5 8 , 6 3 , 6 4 , 7 1 − 7 6 a l g inate, 37,38,40,48,62,77−80 fibrin, 29,33,81,82 platelet-rich plasma, 61,83 hyaluronic acid, 27,31,57,84,85 gellan gum, 86 chitosan, 42,87 and sugar cane biopolymer. 88 For 10% of studies, synthetic materials included oligo(poly(ethylene glycol) fumarate) (OPF), 43,46,89 poly(N-isopropylacrylamide-co-acrylic acid) (poly(NiPAAm-co-AAc)), 90 poly(L-lactide-co-3-caprolactone) (PLCL), 91,92 Si-HPMC, 36 polypeptides, 35,65,93 and α-CD-EG 4400. 94 The scaffolds using combined materials were composed by two or more natural materials, representing 51% of the studies, 17−19,49,60,66−68,95−100 by association of two or more synthetic polymers, 101−109 by the association of natural materials with a synthetic polymer, 20,23,44,45,51,54−56,59,76,110−128 128 by association of others materials.…”

Hydrogel of polysaccharide of sugarcane molasses as carrier of bone morphogenetic protein in the reconstruction of critical bone defects in rats

“…In rats that remained with the biopolymer in the presentation of gel, screens and suture wires, no side effects were detected. Comparative observational studies in animals, between the polymer and other materials, commonly used in humans, although even without data for completion of statistical studies, showed the biopolymer superiority [16][17][18][19][20][21] .…”

Bacterial cellulose an effective material in the treatment of chronic venous ulcers of the lower limbs

“…In this scenario, bioengineering gains space by associating the use of biomaterials, cell culture and growth factors in the production of instruments aimed at curing skin lesions [ 8 ]. Recently, Bacterial Cellulose (BC), a biomaterial produced from biotechnological synthesis, has gained prominence by achieving promising results when used as a dressing and biological graft [ 9 , 10 ].…”

“…BC is produced from the action of the bacterium Zoogloea SP on the substrate of sugarcane molasses [ 9 ], manufactured by POLISA, Biopolymers for Health, a startup hosted by the Federal Rural University of Pernambuco (UFRPE). It is an exopolysaccharide and composed of several monosaccharides, glucose (87.57%), xylose (8.58%), ribose (1.68), mannose (0.82%), arabinose (0.37), galactose (0.13%), raminose (0.01%), fructose (0.01) and glucuronic acid (0.83) [ 11 , 12 ].…”