Effects of broccoli extract on biodistribution and labeling blood components with 99mTc-GH

Abstract

PURPOSE: People consume vegetables without the knowledge of the side effects of the biological and chemical contents and interactions between radiopharmaceuticals and herbal extract. To this end, current study is focused on the effects of broccoli extract on biodistribution of radiolabeled glucoheptonate (99mTc-GH) and radiolabeling of blood components. METHODS: GH was labeled with 99mTc. Quality control studies were done utilizing TLC method. Biodistribution studies were performed on male rats which were treated via gavage with either broccoli extract or SF as control group for 15 days. Blood samples were withdrawn from rats' heart. Radiolabeling of blood constituents performed incubating with GH, SnCl2 and 99m Tc. RESULTS: Radiochemical yield of 99mTc-GH is 98.46±1.48 % (n=8). Biodistribution studies have shown that according to the control, the treated group with broccoli has approximately 10 times less uptake in kidney. The percentage of the radioactivity ratios of the blood components is found to be same in both groups. CONCLUSIONS: Although there is no considerable effect on the radiolabeling of blood components, there is an outstanding change on the biodistribution studies especially on kidneys. The knowledge of this change on kidney uptake may contribute to reduce the risk of misdiagnosis and/or repetition of the examinations in Nuclear Medicine.

Anti toxic effect of broccoli extract on stannous dichloride toxicity

“…The procedure used for HPLC for broccoli extract was similar to that reported in other studies 19 . A low pressure gradient HPLC system (LC-10ATvp quaternary pump, SPD-10A/V UV detector, RF-10AXL Fluorescence detector, RAD501 single channel analyzer, a syringe injector equipped with a 1 mL loop and 7-µm VP 250/21 Nucleosil 100-7 C18 column (MachereyNagel)) was used for the analytical experiments.…”

“…A similar procedure to that for the extraction of broccoli has been applied as described previously 19 . Broccoli was purchased from local market, dried at room temperature and powdered.…”

Evaluation of biologic effects of an Ilex paraguariensis aqueous extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

“…Data have demonstrated the effects of synthetic and natural drugs on this radiolabeling process (Mousinho et al, 2008;Holanda et al, 2009;Bustani et al, 2009;Rocha et al, 2009;De et al, 2009;Cekic et al, 2011;Souza et al, 2011). In consequence, the labeling of blood constituents with 99m Tc has been used as an in vitro assay to screening effects of synthetic or natural products that could have actions related to the band-3 and calcium channels or antioxidant/oxidant properties.…”

Forced Swim Alters the Radiolabeling of Blood Constituents from Wistar Rats

“…In one case report, 99m Tc RBCs were used to localize intrathoracic bleeding [24]. Authors reported that synthetic and natural medications might alter the bioavailability of radiopharmaceuticals [25,26], as well as the radiolabeling of blood constituents [25,[27][28][29][30].…”