Evaluation of optical density of bone defects filled with calcium phosphate cement and bioactive glass in rats

Abstract

Purpose: To evaluate new bone formation, by the analysis of optical density, in rat femoral defects filled with calcium phosphate cement (CPC) and bioactive glass (BG). Methods: Twenty-one rats were divided into three groups, Group I (CPC), Group II (BG), and Group III (control), and assessed after 7, 15, and 30 days. Three bone cavities were made in the left femur and filled with CPC, BG, and no material (control). Digital images were obtained and the results were subjected to statistical analysis of variance (ANOVA), complemented by the Friedman and Kruskal-Wallis nonparametric tests, with a significance level of 5%. Results: Regarding optical density, Group I showed statistical values significantly higher than Group III and also higher, although not statistically significant, than Group II, in all observation periods. When Groups II and III were compared, Group II showed higher optical density values, without statistically significant differences, in all periods. Conclusion: The biomaterials analyzed showed higher optical density in relation to the control group in all observation periods, calcium phosphate cement being the best option in the repair of bone defects, but without statistically significant differences in relation to bioactive glass.

Comparative Study on Use of Platelet-Rich Plasma Alone and in Combination With Alpha-Tricalcium Phosphate Cement for Bone Repair in Rats

“…The histological analyses also demonstrated that there was no inflammatory response or necrosis in any of the animals, and this is concordant with studies that aimed to assess the biocompatibility of α-TCP cement 39 , 40 . Another possible explanation for the absence of inflammation or bone necrosis is that saline solution was used to constantly irrigate the surgical field while the defects were being created, and that both the cement and the surgical materials used in all the procedures had been sterilized.…”

Estudo comparativo do uso isolado de plasma rico em plaquetas e combinado com cimento de alfa-fosfato tricálcico no reparo ósseo em ratos

“…Studies have proven that combining PRP with biomaterials optimizes and accelerates the bone tissue regeneration process (38) . The histological analyses also demonstrated that there was no inflammatory response or necrosis in any of the animals, and this is concordant with studies that aimed to assess the biocompatibility of α-TCP cement (39,40) . Another possible explanation for the absence of inflammation or bone necrosis is that saline solution was used to constantly irrigate the surgical field while the defects were being created, and that both the cement and the surgical materials used in all the procedures had been sterilized.…”

Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo

“…Pre-operatively, rats were anesthetized with tiletamine zolazepam (Virbac Korea, Seoul, Korea) and xylazine HCl (Bayer Korea, Kyungkido, Korea). Rats were divided into five groups: Control (defect alone), Vetbond (3M, St. Paul, USA), CMP, HA, and HA-col. For evaluation of osteoconductive potency, a 2-mm diameter cortical bone defect was created in the diaphysis of the femur using a dental drill [33] , followed by the implantation of each of the prepared bioceramics including CMP, HA, and HA-col in the lesion. Defects were carefully and sufficiently lavaged using 0.9% saline.…”