The effect of copaiba balsam on Walker 256 carcinoma inoculated into the vagina and uterine cervix of female rats

Brito, Nara Macêdo Botelho; Brito, Marcus Vinicius Henriques; Carvalho, Rita de Kássia Vidigal; Matos, Lia Tavares de Moura Brasil; Lobato, Rodolfo Costa; Corrêa, Suelen Costa; Brito, Rosângela Baía

doi:10.1590/s0102-86502010000200010

Cited by 12 publications

(9 citation statements)

References 16 publications

(6 reference statements)

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“…Activity of different extracts of copaiba on the immune system is poorly defined in the literature. Brito et al [8] suggests that the plant oil-resin may operate with an immune response modulator by stimulating or inhibiting their functions according to its compounds concentration. In fact, the authors demonstrated that in vaginal Walker tumor model and the uterine cervix, the oil-resin treatment did not reduced the tumor growth and had non-effect on infiltrating immune cells (T lymphocytes and NK cells) [8,9].…”

Section: Introdutionmentioning

confidence: 99%

“…Brito et al [8] suggests that the plant oil-resin may operate with an immune response modulator by stimulating or inhibiting their functions according to its compounds concentration. In fact, the authors demonstrated that in vaginal Walker tumor model and the uterine cervix, the oil-resin treatment did not reduced the tumor growth and had non-effect on infiltrating immune cells (T lymphocytes and NK cells) [8,9]. In the present study, we evaluated the antitumor and immunomodulatory effect of ethanolic extracts obtained from copaiba bark using the model of subcutaneous growth of Ehrlich carcinoma.…”

Section: Introdutionmentioning

confidence: 99%

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Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Albiero¹,

Nery²,

Kelly³

et al. 2016

IOSR

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Oil-resin of Copaifera multijuga Hayne is popularly used due to its anti-inflammatory properties. In this study, we evaluate the effects of the bark ethanolic extract on Ehrlich tumor cells. For extract preparation, bark was removed from stem and chopped into smaller pieces. Most were dried and grounded. The first extract was made with hexane, followed by ethyl acetate. Absolute ethanol was added to the residue and extract was filtered and concentrated (EtOH). In vitro cytotoxic activity of the EtOH was analyzed on both spleen and tumor cells. For evaluation of in vivo activity, Ehrlich carcinoma bearing Swiss mice were daily treated with different concentration of EtOH during 30 days. Evaluation of in vitro cell viability showed that 1mg/ml extract is cytotoxic to spleen and tumor cells. Evaluation of in vivo tumor development showed that treatment with 200mg/kg of EtOH was able to reduce in 45.28% the tumor growth. Treatment for 7 days increased IL-12p70, IFN-γ and TNF-α production and for 14 days reduced the lymphoproliferative activity in both tumor bearing and mice healthy. Our results show that the ethanolic extract of copaiba bark affect Ehrlich tumor cells, reducing their cell viability in vitro as well as their development in vivo.

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Section: Introdutionmentioning

confidence: 99%

Section: Introdutionmentioning

confidence: 99%

Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Albiero¹,

Nery²,

Kelly³

et al. 2016

IOSR

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“…Pode-se dizer então, que o óleo de copaíba atuou como potencializador do efeito carcinogênico da doxorrubicina em Drosophila melanogaster nas concentrações 0,5% e 1%. Brito et al (2010) também evidenciaram estimulação de crescimento tumoral pelo óleo de copaíba da espécie Copaifera officinalis em ratas com carcinoma de Walker 256. No estudo foi verificado crescimento tumoral de cerca de 70% quando utilizada a dose de 4,8 mL/kg do óleo de copaíba.…”

Section: Resultsunclassified

Efeito modulador do óleo de copaíba (Copaifera officinalis L.) sobre a carcinogenicidade da doxorrubicina, avaliado por meio do teste para detecção de clones de tumores em Drosophila melanogaster

Maciel

Magalhães

Orsolin

2017

cmbio

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Introdução: o óleo de copaíba é o composto extraído do tronco da copaibeira (Copaifera sp.) que tem sido utilizado na medicina popular desde a chegada dos portugueses ao Brasil. Objetivo: o objetivo deste estudo foi avaliar possíveis efeitos carcinogênicos e/ ou anticarcinogênicos do óleo de copaíba (Copaifera officinalis L.), por meio do teste para detecção de clones de tumores epiteliais (warts) em Drosophila melanogaster. Metodologia: foram preparadas três soluções de óleo de copaíba, nas proporções de 0,5%, 1% e 2%. Nessas soluções foram cultivadas Drosophilas melanogaster expostas simultaneamente à doxorrubicina na concentração de 0,4 mM, agente conhecidamente cancerígeno, também utilizado como controle positivo na presente pesquisa. Para controle negativo foi utilizado Tween 80 (1%). O tratamento foi realizado com todas as larvas descendentes do cruzamento de fêmeas wts/ TM3 com machos mwh/mwh. Resultados: o óleo de copaíba apresentou atividade carcinogênica quando utilizado isoladamente na concentração 2%, visto que houve aumento estatisticamente significativo (p ≤ 0,05) na frequência de tumores em comparação com o controle negativo. Além disso, evidenciou-se potencialização do efeito carcinogênico da doxorrubicina nas concentrações 0,5% e 1%, uma vez que houve um aumento estatisticamente significativo (p ≤ 0,05) na frequência de tumores nessas concentrações, quando associadas à DXR, em comparação com o controle positivo. Conclusão: os resultados evidenciaram o efeito carcinogênico isolado do óleo de copaíba, bem como seu efeito potencializador quando associado à doxorrubicina

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“…Although the improvement in NK activity seems to be opposite to the anti-inflammatory effect of copaiba, it is important to note that the biological effect of the plant extract reflects the complexity of the compounds and their different mechanisms of action [9,26]. Thus, reduction of Ehrlich tumor growth may be a result of the association of the anti-inflammatory and immunostimulatory effects.…”

Section: /8mentioning

confidence: 99%

Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

Rodrigues

Albiero

Nery

et al. 2019

Sci Med

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AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.

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The effect of copaiba balsam on Walker 256 carcinoma inoculated into the vagina and uterine cervix of female rats

Cited by 12 publications

References 16 publications

Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Efeito modulador do óleo de copaíba (Copaifera officinalis L.) sobre a carcinogenicidade da doxorrubicina, avaliado por meio do teste para detecção de clones de tumores em Drosophila melanogaster

Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

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