Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

Abstract

PURPOSE: To validate Peyer's patch as an anatomical repair landmark for colorectal surgery in rats and to measure the collagen content in segments of the colon containing or not containing Peyer's patch. METHODS: The distance between Peyer's patch and the peritoneal reflection was measured in forty-five Wistar rats. The colon and rectum were resected for quantification of collagen content by means of computer-assisted image analysis in regions of the colon with and without Peyer's patch. RESULTS: There was great variation in the distance between Peyer's patch and the peritoneal reflection when the male and female rats were considered as a single group (p=0.04). Comparison between the genders showed that the distance between the patch and the peritoneal reflection was greater in female than in male rats (p=0.001). The colonic segment containing Peyer's patch was observed to have lower tissue collagen content than the segment in which this structure was not present (p=0.02). CONCLUSION: Peyer's patch can be indicated as an anatomical repair landmark, and there is a need to study the healing of colorectal anastomoses in rats based on differing quantities of tissue collagen existing in the colonic wall with or without this structure.

Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury

“…The collagen content of the Peyer’s plaque is lower compared to other parts of the colon. The collagen amount can affect the healing process of the anastomosis line 32 . We did not take into consideration the location of the Peyer’s plaque while performing the resection and end-to-end anastomosis.…”

Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats

“…In the Ischemia and HBOT/Ischemia groups, ischemia was induced as follows: Peyer's patch and the peritoneal reflection were identified 15 as the landmark for the procedure. A colon segment 2 cm cranial to the landmark was isolated and the venous-arterial branches that drained or irrigated this left colonic segment were electrocoagulated.…”