Fibrin adhesive and the vaginal vault synthesis on female rabbits abdominal hysterectomies

Abstract

Purpose:To investigate the effectiveness of fibrin glue in comparison with polyglycolic acid suture to promote the closure of rabbit's vaginal vault, after abdominal hysterectomy. Methods: Twenty female, adults, New Zealand rabbits, were submitted to abdominal hysterectomy and randomly distributed to polyglycolic acid suture (G-PA / n=10) or fibrin glue closure of vaginal vault (G-FG / n=10). Radiograph study allowed identifying vault vaginal suture disrupter or fistulas to urinary bladder or rectum. Videovaginoscopy study allowed identifying the presence of cellulites, abscess formation, tissue granulation or granuloma. Vaginal cuff burst test allowed to identify by the escape of air bubbles and rupture pression record. Histological sections stained with Picrosirius red allowed the measure of fibrous tissue healing. Results: The videovaginoscopy identified a significant difference (Fisher Test p<0.3142) of granulation tissue in the animals of G-PA (40%) in comparison with the G-FG (20%). The gross inspection showed the same relation in the granulation tissue occurrence (Fisher test p< 0.1749) with G-PA (50%) and G-FB (20%). The visceral adhesion to the vaginal vault wound was statistical significant (Fisher test p< 0.1749) with G-PA (50%) and G-FG (20%). The pressure of rupture (mm Hg) of the burst test was similar (p<0.0421) in the animals of G-PA (61.5±19.3) and G-FG (72.5±21.9). The collagen matrix of vault wound healing was similar (p< 0.0231) between the G-PA (31.63±15) and the G-FG (23.2±13.2). Conclusion: The vaginal vault closure using the fibrin glue is a safe and reliable procedure after abdominal hysterectomy in female rabbit model.

Sellantes de fibrina en ginecología laparoscópica. Aspectos moleculares y mecanismo de acción: revisión de la literatura

“…El liofilizado necesario para obtener 1 ml de solución sellante de fibrina contiene los siguientes principios activos: proteína coagulable 75-115 mg; fibrinógeno 70-110 mg; plasmafibronectina 2-9 mg; plasminógeno 40-120 mcg; factor XIII 10-50 UI; solución de aprotinina bovina we3.000 UIC/ml; trombina humana liofilizada; trombina 4: 1 ml de la solución reconstituida contiene 4 UI; trombina 500: 1 ml de la solución reconstituida contiene 500 UI; solución de cloruro de calcio 40 µmol CaCI 2 /m. 5,[11][12][13][14] Características biofísicas Las formas de fibrinógeno fotoquímicamente reticulado de los sellantes de tejidos son al menos 5 veces más fuertes (presión), (95,9 +/-4,5 kilopascales (kPa) o 19,501 g) que un sellador de fibrina convencional (17,9 +/-9,3 kPa o 3,640 g); con una fuerza tensil de 125 g/cm². [15][16][17][18] Cuando se comparan las características de varios agentes hemostáticos, se encuentra que las suturas son las que ofrecen mayor presión y pueden ser utilizadas en vasos de mayor calibre (900 mmHg y 7 mm, respectivamente), seguidos por los clips de polímero (854 mmHg y 4 mm), bipolar (601 mmHg y 4 mm), endograpadora (310 mmHg y 17 mm) y luego los sellantes de fibrina en parénquima (378 mmHg), superiores al uso de monopolar (230 mmHg) bisturí armónico (204 mmHg y 4 mm).…”

Perioperative care in an animal model for training in abdominal surgery: is it necessary a preoperative fasting?

“…In the last 10 years, the medical science has been noticing even more the potential of the white rabbit (Oryctolagus cuniculus) of the New Zealand lineage 1 as an experimental animal model for the research in video-surgery, general surgery, orthopedics, gynecology, vascular, craniofacial and urologic [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] . However, there are few information with a good scientific evidence, regarding the perioperative care [2][3][4][5][6][7][8][9][10][11][12][13][14][15]17 .…”

“…However, there are few information with a good scientific evidence, regarding the perioperative care [2][3][4][5][6][7][8][9][10][11][12][13][14][15]17 .…”

“…For abdominal surgical procedures through laparotomy, it was proposed in several studies different anesthetic protocols for many isolated or combined routes: inhalation, endovenous, epidural and intramuscular, depending on the effects related to the anesthetic used and the physiological and behavioral parameters related to the rabbits 2,[4][5][6][7][8][9][10][11][12][13][14][15]17,[22][23][24] .…”

“…Methods of euthanasia may impact the study results, either directly with qualitative or quantitative changes of a parameter to be measured, or even generating histological changes, or indirectly, due to the circumstances associated to the technique, handling and exposure to other animals killed 22 . Most studies adopts as a technique of euthanasia the injection of overdose of barbiturates, for instance, pentobarbital sodium 2,7,9,10,23 , or injection of potassium chloride 6,8 , up to a recognition of the animal's death, through the absence of heartbeats, breathing movements and reflexes.…”