Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion

Tucci, Sílvio; Molina, C.; Cologna, Adauto José; Suaid, Haylton Jorge; Tirapelli, Luís Fernando; Celini, Fábia Martins; Martins, Antônio Carlos Pereira

doi:10.1590/s0102-86502008000700006

Cited by 6 publications

(4 citation statements)

References 22 publications

(26 reference statements)

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“…Macroscopically, at 8 days, in all the groups, the size of the obstructed kidney was found to be slightly increased compared with that of the contralateral kidney. Tucci-Junior et al 10 demonstrated that this increase in size is due to increased mitochondrial respiration activity as a compensatory mechanism of the ischemic organ.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Two-dimensional and color Doppler ultrasound examinations were performed in 16 randomly selected animals at 7 days before the surgical procedure to determine patterns of normality of the structural organ and hemodynamics using an ultrasound equipment (GE Logiq-E) with sector transducer 8C (4)(5)(6)(7)(8)(9)(10)(11). In these animals, color Doppler ultrasound was also performed after the placement and removal of the vascular clip, allowing the assessment of renal ischemia and reperfusion.…”

Section: Assessment Using Two-dimensional and Color Doppler Ultrasoundmentioning

confidence: 99%

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Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window

Monteiro

Santos²,

Almeida³

et al. 2018

Acta Cir. Bras.

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To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell Methods: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphatebuffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies. Results: Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury. Conclusion: The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Assessment Using Two-dimensional and Color Doppler Ultrasoundmentioning

confidence: 99%

Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window

Monteiro

Santos²,

Almeida³

et al. 2018

Acta Cir. Bras.

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“…Additionally, compensatory renal hypertrophy, a phenomenon already well established in the literature may have occurred 24 .…”

Section: Introductionmentioning

confidence: 85%

Effect of glutamine on the total antioxidant system of rats subjected to renal ischemia and reperfusion

et al. 2011

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PURPOSE: To evaluate the protective effects of glutamine administered before renal ischemia-reperfusion on plasma antioxidant protection, and lung and renal tissue injury. METHODS: 33 rats underwent right nephrectomy. On the eighth postoperative day, animals were randomized into three groups (n=11): glutamine, control and sham. Each group of animals received, by gavage, a particular diet for 7 days. On day 14 following nephrectomy, the animals were subjected to left renal ischemia-reperfusion. After this, blood samples were collected and the animals were killed. At necropsy the kidney and lung were removed for histology. RESULTS: The levels of total antioxidant capacity were higher in the glutamine group and control group compared with the sham group. The levels of glutathione peroxidase in both the sham and glutamine groups were higher when compared with the control group (p<0.05). The level of superoxide dismutase in the sham group was clearly higher than that in the glutamine and control groups. Histological examination showed no differences between the three groups. CONCLUSION: Prior intake of glutamine improves total antioxidant capacity and increases glutathione peroxidase levels in rats subjected to renal ischemia-reperfusion.

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“…A protective effect has also been observed with the use of verapamil 3 . However, no variation in ATP production was observed in the analysis of mitochondrial function of rat kidneys obtained during the development of compensatory renal hypertrophy (which causes an increased renal blood flow) followed by ischemia and reperfusion 4 .…”

Section: Introductionmentioning

confidence: 95%

Lovastatin protects mithochondrial and renal function in kidney ischemia-reperfusion in rats

Tucci¹,

Molina²,

Cassini³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To investigate the effect of lovastatin on renal ischemia followed by reperfusion. METHODS:Thirty one Wistar rats submitted to left renal ischemia for 60 minutes followed by contralateral nephrectomy were divided into two groups: A (n =17, control, no treatment), and B (n=14, lovastatin 15 mg/kg/day p.o. ten days before ischemia). The animals were sacrificed at the end of ischemia, after 24 hours and at seven days after reperfusion. Survival, serum urea and creatinine levels and renal mitochondrial function were evaluated. RESULTS:Mortality was 29.4% in group A and 0.7% in group B. Urea and creatinine levels were increased in both groups, but the values were significantly lower in group B. Mitochondrial function showed decoupling in 83.4% of group A, as opposed to 38.4/% of group B. CONCLUSIONS:The result shows a protective action of renal function by lovastatin administered before ischemia/reperfusion. Since most of the mitochondrial fraction presented membranes with the ability to maintain ATP production in group B, stabilization of the mitochondrial membrane should be considered as part of the protective action of lovastatin on renal function in ischemia/reperfusion. Key words: Lovastatin. Mitochondria. Reperfusion Injury. Ischemia. Rats. RESUMO OBJETIVO:Investigar a ação da lovastatina na isquemia renal seguida de reperfusão. MÉTODOS:Trinta e um ratos Wistar submetidos à isquemia renal esquerda durante 60 minutos, seguida da nefrectomia contralateral, foram distribuídos em dois grupos: A (n=17, controle, sem tratamento) e B (n=14, recebendo 15 mg/Kg/dia de lovastatina via oral), durante os dez dias que antecederam a isquemia. Os animais foram mortos ao final da isquemia, e com 24 horas e sete dias após a reperfusão. Foram avaliadas a sobrevida, os valores séricos de uréia e creatinina e a função mitocondrial renal. RESULTADOS:A mortalidade foi 29,4% no grupo A e 0,7% no grupo B. Os níveis de uréia e creatinina elevaram-se nos dois grupos, mas foram significativamente menores no grupo B. No grupo A a função mitocondrial renal ficou desacoplada em 83,4% dos ensaios, enquanto que no grupo B isto ocorreu em apenas 38,4% dos ensaios. CONCLUSÕES:Os resultados mostram que a administração de lovastatina antes do episódio de isquemia protege a função renal. No grupo B, como a maior parte da fração mitocondrial isolada apresentou função acoplada à produção de ATP, deve-se também considerar a estabilização da membrana mitocondrial como parte da ação protetora da lovastatina na função renal durante isquemia e reperfusão.

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Compensatory renal growth and mitochondrial function: the influence of warm ischemia and reperfusion

Cited by 6 publications

References 22 publications

Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window

Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window

Effect of glutamine on the total antioxidant system of rats subjected to renal ischemia and reperfusion

Lovastatin protects mithochondrial and renal function in kidney ischemia-reperfusion in rats

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