Rosiglitazone-enriched diet did not protect liver ischemia-reperfusion injury in a rat model

Abstract

PURPOSE: To determine whether rosiglitazone-enriched diet offer protection in a classical model of liver ischemia-reperfusion injury in rats. METHODS: Two days before the experiment, rats were divided into 2 groups: Control Group (n=13) rats fed with standard diet; Rosi Group (n=13): rats fed with a powdered standard diet supplemented with rosiglitazone. The animals were submitted to liver ischemia-reperfusion by clamping the pedicle of median and left anterolateral lobes. After 1 hour of partial hepatic ischemia, the clamp was removed for reperfusion. After 2 or 24 hours (Control and Rosi Groups), blood was collected for enzymes and cytokines analysis. Ischemic and non-ischemic liver were collected for malondialdehyde analysis and histological assessment. Lungs were removed for tissue myeloperoxidase quantification. RESULTS: There were no statistical differences between groups for all analysed parameters. CONCLUSION: In this model, rosiglitazone-enriched diet did not protect liver against ischemia-reperfusion injury.

Low-power laser irradiation fails to improve liver regeneration in elderly rats at 48 h after 70 % resection

“…The capacity of the liver to regenerate is an important clinical issue after major hepatectomies and certainly makes the difference between life and death in some cases of postoperative malfunction when the liver remnant is too small or has an impaired regenerative capacity [1][2][3][4][5][6]. In this sense, several approaches have been tested to stimulate liver regeneration after a massive resection and in situations in which the liver remnant may be too small; however, they have produced controversial results [4,5,[7][8][9].…”

Efeitos do N2-Mercaptopropionilglicina na isquemia e reperfusão hepática em ratos

“…8 Considerando a importância e gravidade das lesões causadas por I/R, diversas drogas foram testadas com o objetivo de minimizar estes efeitos. 7,29,[31][32][33][34][35][36][37][38][39][40][41][42][43] No cenário das moléculas capazes de interferir no processo deletério de I/R apresenta-se o N2-mercaptopropioniglicina (N2-MPG), um composto tiólico sintético, que exerce efeitos benéficos contra as lesões de reperfusão através de suas propriedades varredoras de radicais livres. 14,30 O efeito protetor do N2-MPG já foi testado com sucesso em I/R em ratos com hipertrofia cardíaca 14 , em enxertos cutâneos de ratos 15 , pancreatite aguda experimental 16 , e isquemia e reperfusão hepática em ratos 7…”