Interleukin-6 (IL-6) influence on colonic anastomosis healing in rats

Abstract

Purpose:To observe the effect of pro-inflamatory citokin, Interleukin-6 (IL-6), administration on the healing of colonic anastomosis in rats. Methods: Thirty Wistar rats were divided into 2 groups of 15 animals each: a control group and an experiment group. The animals of the experiment group were submitted to injection of 500µg/kg of IL-6 Recombinant (Sandoz Phamarceutical ® ) subcutaneously 24 and 12 hours preceding the surgery, and the control group, were submitted to the injection of saline solution, using the same volume of solution as well as the way of administration. The rats were submitted to an end to end colonic anastomosis, being sacrificed in the 7 th post-operative day, the site of the anastomosis was evaluated regarding to the adhesions that was quantified and the presence of complications was observed. The colon containing the anastomosis was submitted to a tensiometric study and a sample were sent for histological studies with HE and Sirius Red dye. Results: The macroscopic and the tensiometric study did not demonstrate any significant differences between the control and experiment group. Regarding the histological evaluation, there was a significant reduction of the chronic inflammatory process in the experiment group (p=0,0209) and a lesser granulation area (p=0,0099). The total amount of collagen was similar in both groups, however, the experimental group showed a predominant area of immature collagen (p=0.009). Conclusions: The longer persistence of the acute phase of wound healing in the experiment group, as well as a decreased of the mature collagen type I, demonstrating that the IL-6 was harmful to the colonic healing anastomosis in rats. Key words: Interleukin-6. Anastomosis, surgical. Rats. RESUMOObjetivo: Verificar a influência da administração da citocina pró-inflamatória, Interleucina-6 (IL-6), na cicatrização de anastomoses colônicas em ratos. Métodos: Utilizaram-se 30 ratos Wistar, divididos em 2 grupos, controle e experimento, de 15 animais cada. Os animais do grupo experimento receberam nas 24 e 12 horas precedentes a cirurgia, 500µg/kg de IL-6 Recombinante (Sandoz Phamarceutical ® ) via subcutâneo, os animais do grupo controle, receberam a administração de soro fisiológico nesse mesmo período, pela mesma via. Foram submetidos à anastomose colônica término-terminal. No 7° dia pós-operatório foram realizadas a eutanásia, com quantificação macroscópica das aderências e visualização de complicações. Em seguida realizou-se o estudo tensiométrico, seguido do estudo histológico das lâminas coradas com Hematoxicilina Eosina e Sirius Red. Resultados: O estudo macroscópico e tensiométrico não demonstraram diferenças significativas entre os grupos estudados. Na avaliação histológica, os resultados significativos demonstraram uma diminuição no grupo experimento do processo inflamatório crônico (p=0,0209) e uma menor área de granulação (p=0,0099) presente neste grupo. Na densitometria do colágeno houve um percentual significativamente maior de colágeno imaturo (tipo III) no grupo ex...

The Humoral Response After Laparoscopic Versus Open Colorectal Surgery: A Meta-Analysis

“…Postoperatively, an early exaggerated IL-6 response has been shown in one study to precede the clinical onset of major complications by 12-48 h [2]. Although this is likely to be effect rather than cause, in experimental animal studies systemic IL-6 administration has been shown to have a direct detrimental effect on the healing of colonic anastomoses [64].…”

The time course of cytokine expressions plays a determining role in faster healing of intestinal and colonic anastomatic wounds

“…Although in the skin the IL-6 levels remained low and constant throughout the study period. Previous studies that have examined the effect of IL-6 on healing process have been ambiguous, with a study indicating a deleterious effect of IL-6 on colon wound healing, which has been shown to prolong the healing process,[ 16 ] whereas other studies have demonstrated the favorable role of IL-6 in skin wound healing, which accelerated the healing process. [ 22 23 ] In human breast cancer patients, the IL-6 levels exhibited a significant jump 1 day after the mastectomy,[ 24 ] which is consistent with our finding where a maximum IL-6 expression was noted 1 day postsurgery.…”

“…[ 13 15 ] Administration of IL-6 to Wister rats has been shown to significantly diminish the chronic inflammatory process in colon tissue and exhibited markedly less granulation within immature collagen formation. [ 16 ] In contrast, in colon anastomotic healing in rats, increased levels of IL-6 were detected, which led to improved healing and mechanical stability during the early postoperative period. [ 17 ] IL-6 levels were also found to be elevated in the lung, liver, and kidney wounds 24 h after anastomosis and a lack of IL-6 expression impaired wound healing.…”

The effect of caffeic acid phenethyl ester and Ankaferd Blood Stopper on the diabetic and nondiabetic gingival wound healing: an experimental study

“…Many studies have reported that various cell sources contribute to the production of wound cytokines [28]. Previous studies examining the effect of IL-6 on the healing process have shown that IL-6 has a negative impact on wound healing by prolonging the colon wound healing process [29]. There are studies showing that IL-6 has a positive role in wound healing, suggesting that it speeds up the healing process in skin wound healing [30].…”