Usefulness of argyrophilic nucleolar organizer regions in detection of lung cells alterations after benzo[a]pyrene instillation

Abstract

PURPOSE: To verify the relationship between AgNOR expression and lung tissues changes of Wistar rats after pulmonary instillation of benzo[a]pyrene (B[a]P). METHODS: Male Rattus norvegicus albinus,Wistar lineage were given a single intrapulmonary instillation of B[a]P at doses of 10 and 20 mg/kg in a volume of approximately 0,3 ml. After 7 and 21 days the rats were killed and the lung slices submitted to a histological technique of AgNOR. AgNOR dots were quantified and the result analyzed by statistical tests; p <= 0,05 was considered significant. RESULTS: The mean values of AgNOR dots for the experimental groups 10/7 (1,51±0,86) and 10/21 (1,84±0,13) were statistically different (p = 0,009). Among the groups 20/7 (1,63±0,11) and 20/21 (2,48±0,28) was observed statistically significant difference (p = 0,003). CONCLUSION: The AgNOR technique can be useful in identification of cells changes induced by B[a]P.

Apoptotic role of natural isothiocyanate from broccoli (Brassica oleracea italica) in experimental chemical lung carcinogenesis

“…Benzo(a)pyrene [(B(a)P)] a major carcinogen in the cigarette smoke is a member of polycyclic aromatic hydrocarbon (PAH) family, and is often used as a model compound for PAH toxicity studies and in animal models of lung cancer (Silva et al, 2006). Equilibrium existing between reactive oxygen species (ROS) formation and endogenous antioxidant defense mechanisms can be disturbed by various chemical agents like B(a)P causing oxidative stress.…”

Emissions generated by sugarcane burning promote genotoxicity in rural workers: a case study in Barretos, Brazil

“…Assuming that the cellular mechanisms for the induction of chromosomal damage are similar in different tissues, the extension of DNA damage in lymphocytes and other tissues most likely reflects the level of cancer risk [12,18]. Furthermore, studies have shown that in Western countries, lung cancer is a major cause of mortality related to malignant tumors, and its pathogenesis involves the accumulation of various molecular abnormalities during long periods of time and has been associated with exposure to PAHs [19-21]. …”

Benzo(a)pireno Em Alimentos

“…O potencial tóxico do Benzo(a)Pireno foi evidenciado em 1951 quando foi aplicado na pele de ratos, produzindo compostos intermediários carcinogênicos que se ligavam a proteínas, DNA e RNA, mostrando ser um potente carcinógeno em modelos de câncer em animais experimentais (Silva et al, 2006). Uma vez ingerido, esse composto tóxico é absorvido rapidamente por diversos órgãos de ratos e camundongos, por ser altamente lipossolúvel, sendo o fígado o órgão que apresenta os maiores níveis desse composto (Meire et al, 2007).…”