Effect of soluble fiber or fructooligosaccharide supplementation upon trinitrobenzenesulphonic acid induced colitis in rats

Murad-Regadas, Sthela M.; Souza, Marcellus H.; Brito, Gerly Anne de Castro; Rodrigues, Lusmar Veras; Regadas, Francisco Sérgio Pinheiro; Vasconcelos, Paulo Roberto Leitão de

doi:10.1590/s0102-86502006000500008

Cited by 7 publications

(12 citation statements)

References 14 publications

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“…In agreement with Taha et al [14], the results of this study indicate a fast coagulation rate (using CyA), although there is still some controversy over its application as a haemostatic agent in internal organs. The rationale for choosing GelSp and TachS as haemostatic agents was that both are commonly used in surgery, and several scientific publications have reported their positive effects [24,25,26].…”

Section: Discussionsupporting

confidence: 92%

“…Wounds that were treated with GelSp and TachS took an average of 80 s to reach full haemostasis; however, in wounds treated with TachS, the bleeding time was shorter. Adhflex® significantly reduced the bleeding time versus the other products studied and on average achieved haemostasis after 25 s. These findings are consistent with data obtained by Taha et al [14], who used other types of CyA in a rabbit model in which liver lesions were created by comparing CyA with absorbable catgut suture (4-0). Here, the haemostasis time was significantly shorter in the group treated with the adhesive.…”

Section: Discussionsupporting

confidence: 89%

“…A previous study [22] used similar timelines, but others used only a single time point [14,23]. In this case, the study design did not allow wound healing to be explored.…”

Section: Discussionmentioning

confidence: 99%

“…Adhesions were quantified in this study [13,14], and untreated injuries showed fewer adhesions. In comparison, the animals treated with Adhflex® had by far the highest scores compared to the other groups.…”

Section: Discussionmentioning

confidence: 99%

“…2 Abdominal adhesions: following euthanasia, the presence or absence of adhesions in the area surrounding the wound was evaluated in each group by the same researcher based on the Peritoneal Adhesion Index (PAI) used by Fontes et al [13] and Taha et al [14] and recently improved by Coccolini et al [15]. Briefly, the PAI is a 4-score grading scale based on the characteristics of abdominal adherence: 0 = no adhesions; 1 = filmy adhesions, blunt dissection; 2 = strong adhesions, sharp dissection, and 3 = very strong and vascularized adhesions, sharp dissection, damage hardly preventable.…”

Section: Methodsmentioning

confidence: 99%

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Behaviour of the Biological Adhesives TachoSil®, GelitaSpon®, and a New Elastic Cyanoacrylate (Adhflex®) in Experimental Renal Trauma and Wound Healing

Lloris‐Carsí

García-Cerdá²,

Prieto‐Moure³

et al. 2016

Eur Surg Res

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Background: Renal injuries are relatively frequent in abdominal trauma. In some cases, adhesives and sealants can be used to repair and preserve injured organs. This paper describes the behaviour of three biomaterials - TachoSil®, GelitaSpon®, and a new elastic cyanoacrylate (CyA), Adhflex® - in standardized experimental renal injuries. Methods: Ninety male Wistar rats (300-350 g) were used. A Stiefel Biopsy Punch (8 mm diameter, 3 mm depth) was used to create injuries to the anterior kidney to evaluate wound healing. The animals were divided into five groups: (1) sham (n = 3); (2) control (n = 6), untreated, standard punch injury created on the anterior left kidney; (3) TachoSil® (n = 27), punch injury treated with TachoSil®; (4) GelitaSpon® (n = 27), punch injury treated with GelitaSpon®, and (5) Adhflex® (n = 27), punch injury treated with the new elastic CyA adhesive. The parameters studied were bleeding time, peritoneal adhesions, and histopathological evaluation of wound healing on days 2, 6, and 18, including measurements of the gap between wound edges, inflammatory reaction (CD68), and vascular neoformation (CD31). Results: The bleeding time was significantly shorter (27.7 ± 12.9 s) in the Adhflex® group than in the control (135.8 ± 11.6 s; p < 0.01), TachoSil® (77.5 ± 7.4 s; p < 0.05), and GelitaSpon® (82.5 ± 14.4 s; p < 0.05) groups. The incidence of intraperitoneal adhesions in the animals treated with Adhflex® was 3.6 times higher than in the non-treated group. It was also higher (p < 0.04) than in the groups treated with TachoSil® and GelitaSpon®. The time point with the largest gap between the wound edges and most abundant granulation tissue was at day 6. The largest gap after 18 days was reported for the Adhflex® adhesive. With regard to the markers CD31 and CD68, Adhflex® showed the largest areas 2 days after surgery, but no differences were found after 6 and 18 days versus the other treatments. The expression of the immunomarkers on the renal samples treated with Adhflex® was consistent with a normal healing process. Conclusions: In this experimental model of renal injuries, the new elastic CyA (Adhflex®) resulted in the shortest bleeding time. It offers rapid sealing of the bleeding produced by renal injuries, fixation to adjacent tissues, and reduced occurrences of relapse. The evolution of the scarring is similar to other procedures. Given that traumatic renal injuries are always an emergency due to haemorrhage, Adhflex® might offer additional benefits over conventional treatment methods in human clinical practice.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

“…A previous study [22] used similar timelines, but others used only a single time point [14,23]. In this case, the study design did not allow wound healing to be explored.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Behaviour of the Biological Adhesives TachoSil®, GelitaSpon®, and a New Elastic Cyanoacrylate (Adhflex®) in Experimental Renal Trauma and Wound Healing

Lloris‐Carsí

García-Cerdá²,

Prieto‐Moure³

et al. 2016

Eur Surg Res

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Fructooligosaccharides exert intestinal anti-inflammatory activity in the CD4+ CD62L+ T cell transfer model of colitis in C57BL/6J mice

et al. 2015

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Therapeutic effect of ginsenoside Rd in rats with TNBS-induced recurrent ulcerative colitis

et al. 2012

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Ulcerative colitis (UC) is characterized by oxidative and nitrosative stress and neutrophil infiltration. In the present study, we aimed to investigate the therapeutic effect of ginsenoside Rd (GRd) in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced recurrent UC. After UC was twice-induced by intracolonic injection of TNBS, rats were intragastrically administered different doses of GRd per day for 7 days. The colonic lesions and inflammation were evaluated both histologically and biochemically. Compared with the TNBS group, GRd treatment facilitated recovery of pathologic changes in the colon after induction of recurrent UC, as evidenced by a significant reduction of colonic weight/length ratio and macroscopic and microscopic damage scores (p < 0.01). The myeloperoxidase and inducible nitric oxide synthase activities with malonyldialdehyde and nitric oxide levels in colonic tissues were significantly decreased in the GRd group compared with those in the TNBS group (p < 0.01). GRd treatment was associated with remarkably increased superoxide dismutase and glutathione peroxidase activities. Results showed a valuable effect of GRd against TNBS-induced recurrent UC by inhibiting neutrophil infiltration and promoting the antioxidant capacity of the damaged colonic tissue.

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Effect of soluble fiber or fructooligosaccharide supplementation upon trinitrobenzenesulphonic acid induced colitis in rats

Abstract: In agreement with other biomedical literature works, the fibrin adhesive is a viable option for the performance of hemostasis in a animal model (rabbit) with severe hepatic injury.

Cited by 7 publications

References 14 publications

Behaviour of the Biological Adhesives TachoSil®, GelitaSpon®, and a New Elastic Cyanoacrylate (Adhflex®) in Experimental Renal Trauma and Wound Healing

Behaviour of the Biological Adhesives TachoSil®, GelitaSpon®, and a New Elastic Cyanoacrylate (Adhflex®) in Experimental Renal Trauma and Wound Healing

Fructooligosaccharides exert intestinal anti-inflammatory activity in the CD4+ CD62L+ T cell transfer model of colitis in C57BL/6J mice

Therapeutic effect of ginsenoside Rd in rats with TNBS-induced recurrent ulcerative colitis

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